公开(公告)号：US20230041450A1

公开(公告)日：2023-02-09

申请号：US17812876

申请日：2022-07-15

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： Changchun Liu ,  Ziyue Li

IPC: C12Q1/6825 ,  C12Q1/6823 ,  G01N27/327

Abstract: Described herein is an immobilization-free, electrochemical method of detecting a target DNA sequence in a sample. The method includes: incubating the sample with a detection mixture, applying an electric field including an alternating current electric field and a direct current offset to the detection mixture to concentrate nucleic acids in the sample and the nucleic acid probe on a positively charged working electrode, wherein a Class 2 Cas protein trans-cleaved electroactive probe is released from the nucleic acid probe when the target DNA is present in the sample and diffuses toward a negatively charged electrode; and measuring the current as potential is applied, wherein detection of a current in the detection mixture indicates the presence of the target DNA sequence in the sample.

公开(公告)号：US12297485B2

公开(公告)日：2025-05-13

申请号：US17812876

申请日：2022-07-15

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： Changchun Liu ,  Ziyue Li

IPC: C12Q1/6825 ,  C12Q1/6823 ,  G01N27/327

Abstract: Described herein is an immobilization-free, electrochemical method of detecting a target DNA sequence in a sample. The method includes: incubating the sample with a detection mixture, applying an electric field including an alternating current electric field and a direct current offset to the detection mixture to concentrate nucleic acids in the sample and the nucleic acid probe on a positively charged working electrode, wherein a Class 2 Cas protein trans-cleaved electroactive probe is released from the nucleic acid probe when the target DNA is present in the sample and diffuses toward a negatively charged electrode; and measuring the current as potential is applied, wherein detection of a current in the detection mixture indicates the presence of the target DNA sequence in the sample.

公开(公告)号：US12110542B2

公开(公告)日：2024-10-08

申请号：US17472300

申请日：2021-09-10

Applicant: University of Connecticut

Inventor： Changchun Liu ,  Xiong Ding

IPC: C12Q1/6855

CPC classification number: C12Q1/6855

Abstract: Disclosed herein are “dual-priming” isothermal amplification method (including “self-priming” and “pairing-priming” strand extension, termed “DAMP”) for rapid nucleic acid detection.

公开(公告)号：US20250154482A1

公开(公告)日：2025-05-15

申请号：US18940920

申请日：2024-11-08

Applicant: University of Connecticut

Inventor： Changchun Liu ,  Jeong Moon

IPC: C12N9/22 ,  C12N15/11 ,  C12Q1/6844

Abstract: The present invention relates to systems and methods for nucleic acid detection powered by CRISPR technology and in particular, an asymmetric CRISPR assay for cascade signal amplification detection of nucleic acids by leveraging the asymmetric trans-cleavage behavior of competitive crRNA.

公开(公告)号：US20220081712A1

公开(公告)日：2022-03-17

申请号：US17472300

申请日：2021-09-10

Applicant: University of Connecticut

Inventor： Changchun Liu ,  Xiong Ding

IPC: C12Q1/6855

Abstract: Disclosed herein are “dual-priming” isothermal amplification method (including “self-priming” and “pairing-priming” strand extension, termed “DAMP”) for rapid nucleic acid detection.

公开(公告)号：US20240084408A1

公开(公告)日：2024-03-14

申请号：US18262667

申请日：2022-02-04

Applicant: University of Connecticut

Inventor： Changchun Liu ,  Kun Yin

IPC: C12Q1/70 ,  B01L3/00

CPC classification number: C12Q1/701 ,  B01L3/502738 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2400/06

Abstract: Described herein is an autonomous, rapid, sensitive, point-of-care target nucleic acid detection system and method based on CRISPR/Cas system. The system and method allow naked eye visualization of multiple target nucleic acid molecules simultaneously in a biological sample in less than an hour. Use of the system and method for multiplex gene diagnosis of SARS-CoV-2 is exemplified.