公开(公告)号：US12297485B2

公开(公告)日：2025-05-13

申请号：US17812876

申请日：2022-07-15

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： Changchun Liu ,  Ziyue Li

IPC: C12Q1/6825 ,  C12Q1/6823 ,  G01N27/327

Abstract: Described herein is an immobilization-free, electrochemical method of detecting a target DNA sequence in a sample. The method includes: incubating the sample with a detection mixture, applying an electric field including an alternating current electric field and a direct current offset to the detection mixture to concentrate nucleic acids in the sample and the nucleic acid probe on a positively charged working electrode, wherein a Class 2 Cas protein trans-cleaved electroactive probe is released from the nucleic acid probe when the target DNA is present in the sample and diffuses toward a negatively charged electrode; and measuring the current as potential is applied, wherein detection of a current in the detection mixture indicates the presence of the target DNA sequence in the sample.

公开(公告)号：US20230041450A1

公开(公告)日：2023-02-09

申请号：US17812876

申请日：2022-07-15

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： Changchun Liu ,  Ziyue Li

IPC: C12Q1/6825 ,  C12Q1/6823 ,  G01N27/327

Abstract: Described herein is an immobilization-free, electrochemical method of detecting a target DNA sequence in a sample. The method includes: incubating the sample with a detection mixture, applying an electric field including an alternating current electric field and a direct current offset to the detection mixture to concentrate nucleic acids in the sample and the nucleic acid probe on a positively charged working electrode, wherein a Class 2 Cas protein trans-cleaved electroactive probe is released from the nucleic acid probe when the target DNA is present in the sample and diffuses toward a negatively charged electrode; and measuring the current as potential is applied, wherein detection of a current in the detection mixture indicates the presence of the target DNA sequence in the sample.