公开(公告)号：WO1993025233A1

公开(公告)日：1993-12-23

申请号：PCT/US1993005640

申请日：1993-06-10

Applicant: UNIVERSITY OF CONNECTICUT ,  PRIMAKOFF, Paul ,  MYLES, Diana, G.

Inventor： UNIVERSITY OF CONNECTICUT

IPC: A61K39/00

CPC classification number: C07K14/705 ,  A61K39/00

Abstract: The subject disclosure relates to methods of contraception, and compositions useful in such methods. Disclosed are methods in which one or more sperm surface proteins are administered to a mammal, the administration being effective in stimulating an immune response whereby antibody which binds to the sperm surface protein is produced in a titer sufficient to prevent or substantially reduce the rate of sperm-egg fusion. Antigenic sperm surface protein peptides are also useful for this purpose.

Abstract translation: 本发明涉及避孕方法，以及在这些方法中有用的组合物。 公开了一种方法，其中向哺乳动物施用一种或多种精子表面蛋白质，该给药有效刺激免疫应答，从而以足以防止或显着降低精子率的滴度产生与精子表面蛋白结合的抗体 -egg融合。 抗原精子表面蛋白质肽也可用于此目的。

公开(公告)号：WO1992022635A1

公开(公告)日：1992-12-23

申请号：PCT/US1992004565

申请日：1992-06-05

Applicant: UNIVERSITY OF CONNECTICUT ,  THE BOARD OF REGENTS acting for and on behalf of ...

Inventor： UNIVERSITY OF CONNECTICUT ,  THE BOARD OF REGENTS acting for and on behalf of ... ,  WU, George, Y. ,  WILSON, James, M. ,  WU, Catherine, H.

IPC: A61K47/48

CPC classification number: A61K48/00 ,  A61K47/645 ,  C12N15/87

Abstract: Molecular complexes for targeting a gene encoding a secretory protein to a specific cell in vivo and obtaining secretion of the protein by the targeted cell are disclosed. An expressible gene encoding a desired secretory protein is complexed to a conjugate of a cell-specific binding agent and a gene-binding agent. The cell-specific binding agent is specific for a cellular surface structure which mediates internalization of ligands by endocytosis. An example is the asialoglycoprotein receptor of hepatocytes. The gene-binding agent is a compound such as a polycation which stably complexes the gene under extracellular conditions and releases the gene under intracellular conditions so that it can function within a cell. The molecular complex is stable and soluble in physiological fluids and can be used in gene therapy to selectively transfect cells in vivo to provide for production and secretion of a desired secretory protein.

Abstract translation: 公开了用于将编码分泌蛋白的基因靶向体内特定细胞并获得目标细胞分泌蛋白质的分子复合物。 编码所需分泌蛋白的可表达基因与细胞特异性结合剂和基因结合剂的缀合物复合。 细胞特异性结合剂对通过内吞作用介导配体内化的细胞表面结构是特异性的。 一个例子是肝细胞的脱唾液酸糖蛋白受体。 基因结合剂是诸如聚阳离子的化合物，其在细胞外条件下稳定地复合基因并在细胞内条件下释放基因，使得其可以在细胞内起作用。 分子复合物稳定并且可溶于生理液体，可用于基因治疗以选择性转染体内细胞以提供所需分泌蛋白的产生和分泌。

公开(公告)号：WO1996040869A1

公开(公告)日：1996-12-19

申请号：PCT/US1996010040

申请日：1996-06-07

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： UNIVERSITY OF CONNECTICUT ,  KRIDER, Hallie, M. ,  BUSHMICH, Sandra, Lee

IPC: C12N05/00

CPC classification number: G01N33/569 ,  G01N33/56911 ,  G01N33/56972 ,  Y02A50/57

Abstract: A method for screening for Lyme borreliosis and other infectious diseases early in the course of the disease are disclosed. It has been found that there is a dramatic difference in surface binding of cultured infectious agents to naturally occurring polymorphonuclear leukocytes (PMNs) (e.g., neutrophils) between infected and noninfected animals, which may be present early in the course of the disease, before measurable antibody response. This binding response can be rapidly and accurately distinguished and quantitated using various detection techniques. The method involves visualizing the surface binding of the cultured infectious agent to naturally-occurring PMN to detect the infection.

Abstract translation: 公开了在疾病过程中早期筛选莱姆硼神经病和其他感染性疾病的方法。 已经发现，在感染和未感染的动物之间，培养的感染因子与天然存在的多形核白细胞（PMN）（例如嗜中性粒细胞）的表面结合之间存在显着的差异，可能在疾病进程之前早期存在，然后可测量 抗体反应。 可以使用各种检测技术快速准确地区分和定量该结合反应。 该方法包括将培养的感染剂与天然存在的PMN的表面结合可视化以检测感染。

公开(公告)号：WO1995014781A3

公开(公告)日：1995-06-01

申请号：PCT/US1994013540

申请日：1994-11-22

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： UNIVERSITY OF CONNECTICUT ,  PADULA, Steven, J.

IPC: C12N15/31

公开(公告)号：WO1994022485A1

公开(公告)日：1994-10-13

申请号：PCT/US1994003648

申请日：1994-04-04

Applicant: UNIVERSITY OF CONNECTICUT ,  ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY

Inventor： UNIVERSITY OF CONNECTICUT ,  ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY ,  WU, George, Y. ,  WU, Catherine, H. ,  CHOWDHURY, Namita, Roy ,  CHOWDHURY, Jayanta, Roy

IPC: A61K47/48

CPC classification number: C12N9/1051 ,  A61K47/645 ,  C12N15/87

Abstract: Molecular complexes for targeting a gene encoding a microsomal enzyme selective for delivery to a cell in vivo or in vitro obtaining production of the enzyme within the targeted cell are disclosed. An expressible gene encoding a desired enzyme is complexed to a conjugate of a cell-specific binding agent and a gene-binding agent. The cell-specific binding agent is specific for a cellular surface structure which mediates internalization of ligands by endocytosis. An example is the asialoglycoprotein receptor of hepatocytes. The gene-binding agent is a compound such as a polycation which stably complexes the gene under extracellular conditions and releases the gene under intracellular conditions so that it can function whithin a cell. The molecular complex is stable and soluble in physiological fluids and can be used in antisense gene therapy to selectively transfect cells in vivo to treat inherited or acquired deficiencies in cellular microsomal enzymes.

Abstract translation: 公开了用于靶向编码选择性用于在体内或体外获得细胞内靶向细胞内产生酶的微粒体酶的基因的分子复合物。 编码所需酶的可表达基因与细胞特异性结合剂和基因结合剂的缀合物复合。 细胞特异性结合剂对通过内吞作用介导配体内化的细胞表面结构是特异性的。 一个例子是肝细胞的脱唾液酸糖蛋白受体。 基因结合剂是在细胞外条件下使基因稳定复合并在细胞内条件下释放基因使其能够在细胞中起作用的化合物，例如聚阳离子。 分子复合物是稳定的并且可溶于生理液体，可用于反义基因治疗以选择性转染体内细胞，以治疗细胞微粒体酶中的遗传或获得性缺陷。

公开(公告)号：WO1992020316A2

公开(公告)日：1992-11-26

申请号：PCT/US1992003875

申请日：1992-05-14

Applicant: UNIVERSITY OF CONNECTICUT ,  TARGETECH, INC.

Inventor： UNIVERSITY OF CONNECTICUT ,  TARGETECH, INC. ,  WU, George, Y. ,  SPITALNY, George, L. ,  FINDEIS, Mark, A.

IPC: A61K0

CPC classification number: C12N15/87 ,  A61K39/12 ,  A61K39/292 ,  A61K47/645 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/6093 ,  C07K16/00 ,  C07K16/082 ,  C12N2730/10134

Abstract: Molecular complexes can be used to target a gene encoding an immunogenic protein or polypeptide to a specific cell in vivo. The gene is incorporated into the target cell, expressed and the gene-encoded product is secreted and an immune response against the immunogenic protein or polypeptide is elicited. The molecular complex comprises an expressible gene encoding a desired immunogenic protein or polypeptide complexed with a carrier of a cell-specific binding agent and a gene-binding agent. The cell-specific binding agent is specific for a cellular surface structure which mediates internalization of ligands by endocytosis. An example is the asialoglycoprotein receptor of hepatocytes. The gene-binding agent is a compound such as a polycation which stably complexes the gene under extracellular conditions and releases the gene under intracellular conditions so that it can function within a cell. The molecular complex is stable and soluble in physiological fluids and can be used to elicit an immune response against a variety of immunogens in an organism for the purpose of vaccination or for the production of antibodies.

Abstract translation: 分子复合物可用于将编码免疫原性蛋白或多肽的基因靶向体内特定细胞。 该基因被并入目标细胞中，表达并且分泌基因编码的产物，并引发针对免疫原性蛋白质或多肽的免疫应答。 分子复合物包含编码与细胞特异性结合剂和基因结合剂的载体复合的所需免疫原性蛋白质或多肽的可表达基因。 细胞特异性结合剂对通过内吞作用介导配体内化的细胞表面结构是特异性的。 一个例子是肝细胞的脱唾液酸糖蛋白受体。 基因结合剂是诸如聚阳离子的化合物，其在细胞外条件下稳定地复合基因并在细胞内条件下释放基因，使得其可以在细胞内起作用。 分子复合物是稳定的并且可溶于生理液体，并且可以用于在生物体内引发针对各种免疫原的免疫应答以用于疫苗接种或产生抗体。

公开(公告)号：WO1996026534A1

公开(公告)日：1996-08-29

申请号：PCT/US1996002174

申请日：1996-02-20

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： UNIVERSITY OF CONNECTICUT ,  OUIMETTE, Donald, R.

IPC: H01J40/14

CPC classification number: H01J31/26 ,  H01J31/38 ,  H01J2201/304 ,  H01J2201/30403 ,  H01J2231/50036 ,  H01J2231/50068

Abstract: Flat panel image sensor (10) is provided by combining photoconductive imaging electrodes of a vidicon with a two-dimensional array of cold cathode field emitters (14) commonly used for flat panel field emission display (FED) systems. The FED operates normally to emit electrons which are accelerated in prior art displays towards a luminescent phosphor to generate light output proportional to the cathode emission. Rather than accelerating towards a phosphor, electrons are accelerated towards a photoconductor layer (16) to replace charge removed from the layer by an incident radiation pattern directed at the photoconductor layer (16) through a layer of transparent, electrically-conducting material (17) which serves as a radiation window. The transparent, electrically-conducting layer (17) may be partitioned to reduce stray capacitance for large area sensors and the partitioned, electrically-conducting layer (17) permits a parallel readout mode of operation.

Abstract translation: 平面图像传感器（10）通过将摄像机的感光成像电极与通常用于平板场发射显示（FED）系统的冷阴极场发射器（14）的二维阵列组合来提供。 FED正常工作以发射在现有技术显示器中朝着发光荧光体加速以产生与阴极发射成比例的光输出的电子。 不是朝向磷光体加速，电子朝向光电导体层（16）加速，以通过指向感光体层（16）的入射辐射图案通过透明导电材料层（17）来替代从层移除的电荷， 其用作辐射窗口。 透明导电层（17）可以被分隔以减小大面积传感器的杂散电容，并且分隔的导电层（17）允许并行读出操作模式。

公开(公告)号：WO1994023050A1

公开(公告)日：1994-10-13

申请号：PCT/US1994003643

申请日：1994-04-04

Applicant: UNIVERSITY OF CONNECTICUT ,  TARGETECH, INC.

Inventor： UNIVERSITY OF CONNECTICUT ,  TARGETECH, INC. ,  CHIOU, Henry, C. ,  INNAIMO, Steven, F. ,  WU, George, Y.

IPC: C12N15/87

CPC classification number: C12N15/1131 ,  A61K47/645 ,  A61K48/00 ,  C12N15/87 ,  C12N2310/12 ,  C12N2310/3513

Abstract: Molecular complexes for targeting a gene encoding an antisense polyribonucleotide to a specific cell in vivo obtaining production of the polyribonucleotide within the targeted cell, and effecting specific inhibition of the expression of cellular or noncellular genes are disclosed. An expressible gene encoding a desired antisense polyribonucleotide is complexed to a conjugate of a cell-specific binding agent and a gene-binding agent. The cell-specific binding agent is specific for a cellular surface structure which mediates internalization of ligands by endocytosis. An example is the asialoglycoprotein receptor of hepatocytes. The gene-binding agent is a compound such as a polycation which stably complexes the gene under extracellular conditions and releases the gene under intracellular conditions so that it can function within a cell. The molecular complex is stable and soluble in physiological fluids and can be used in antisense gene therapy to selectively transfect cells in vivo or in vitro to provide for production of the antisense polyribonucleotide within the targeted cell, and inhibition of the expression of cellular or noncellular genes.

Abstract translation: 分子复合物用于将编码反义多核糖核苷酸的基因靶向特定细胞体内获得靶向细胞内的多核糖核苷酸的产生，并且对细胞或非细胞基因的表达进行特异性抑制。 编码所需反义多核糖核苷酸的可表达基因与细胞特异性结合剂和基因结合剂的缀合物复合。 细胞特异性结合剂对通过内吞作用介导配体内化的细胞表面结构是特异性的。 一个例子是肝细胞的脱唾液酸糖蛋白受体。 基因结合剂是诸如聚阳离子的化合物，其在细胞外条件下稳定地复合基因并在细胞内条件下释放基因，使得其可以在细胞内起作用。 分子复合物是稳定的并且可溶于生理液体，可用于反义基因治疗，以在体内或体外选择性转染细胞，以提供靶向细胞内反义多核糖核苷酸的产生，抑制细胞或非细胞基因的表达 。

公开(公告)号：WO1993004701A1

公开(公告)日：1993-03-18

申请号：PCT/US1992007339

申请日：1992-09-04

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： UNIVERSITY OF CONNECTICUT ,  WU, George, Y. ,  WU, Catherine, H.

IPC: A61K48/00

CPC classification number: C12N15/87 ,  A61K47/645 ,  A61K48/00 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3513

Abstract: Molecular complexes for targeting oligonucleotides, such as antisense oligonucleotides or ribozymes, to a specific cell to block expression of a gene or genes in the cell are described. The single-stranded poly- or oligonucleotide is complexed to a conjugate of a cell-specific binding agent and a poly- or oligonucleotide-binding agent. The cell-specific binding agent is specific for a cellular surface structure which mediates internalization of the complex. An example is the asialoglycoprotein receptor of hepatocytes. The poly- or oligodeoxy-nucleotide-binding agent is a compound such as a polycationic protein which stably complexes the oligonucleotide under extracellular conditions and releases it under intracellular conditions so that it can hybridize with the target RNA. The molecular complex is stable and soluble in physiological fluids and can be used to selectively introduce antisense oligonucleotides, ribozymes or other signal-stranded oligonucleotides into a cell to inhibit expression of a gene within the cell. The oligonucleotide can be directed against cellular genes (e.g., cellular oncogenes) or genes of noncellular origin (e.g., viral oncogenes, genes of an infecting pathogen).

Abstract translation: 描述了用于将寡核苷酸（例如反义寡核苷酸或核酶）靶向特异性细胞以阻断细胞中基因或基因表达的分子复合物。 单链多核苷酸或寡核苷酸与细胞特异性结合剂和多核苷酸或寡核苷酸结合剂的缀合物复合。 细胞特异性结合剂对于介导复合物内在化的细胞表面结构是特异性的。 一个例子是肝细胞的脱唾液酸糖蛋白受体。 聚 - 或寡脱氧核苷酸结合剂是一种化合物，例如聚阳离子蛋白质，其在细胞外条件下稳定地将寡核苷酸复合，并在细胞内条件下将其释放，使得其可以与靶RNA杂交。 分子复合物是稳定的并且可溶于生理液体，可用于选择性地将反义寡核苷酸，核酶或其它信号链寡核苷酸引入细胞中，以抑制细胞内基因的表达。 寡核苷酸可以针对细胞基因（例如，细胞癌基因）或非细胞起源的基因（例如，病毒癌基因，感染病原体的基因）。

公开(公告)号：WO1992005250A1

公开(公告)日：1992-04-02

申请号：PCT/US1991006885

申请日：1991-09-23

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： UNIVERSITY OF CONNECTICUT ,  WU, George, Y. ,  WU, Catherine, H.

IPC: C12N15/00

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A61K47/645 ,  A61K47/6807 ,  A61K48/00 ,  C12N15/87

Abstract: The expression of polynucleotide introduced into a cell by means of a targeted complex of the polynucleotide linked to a cell-specific binding agent can be prolonged by stimulating the cell to replicate.

Abstract translation: 通过与细胞特异性结合剂连接的多核苷酸的靶向复合物引入细胞的多核苷酸的表达可以通过刺激细胞复制而延长。