公开(公告)号：WO2016154620A1

公开(公告)日：2016-09-29

申请号：PCT/US2016/024519

申请日：2016-03-28

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： VARADARAJAN, Navin ,  ROMAIN, Gabrielle ,  LIADI, Ivan ,  SENDRA, Victor, G. ,  ROYSAM, Badrinath

IPC: G01N33/58 ,  G01N33/52 ,  C12Q1/68

CPC classification number: C12Q1/68 ,  G01N33/56966

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

Abstract translation: 这里提出的是通过以下方式评价细胞活性的方法：将细胞群置于一个区域上; 测定细胞群体的动态行为作为时间的函数; 基于动态行为识别感兴趣的细胞; 表征细胞的分子轮廓; 并将获得的信息相关联。 测定的动态行为可以包括细胞激活，细胞抑制，细胞相互作用，蛋白质表达，蛋白质分泌，细胞增殖，细胞形态学变化，运动性，细胞死亡，细胞毒性，细胞裂解及其组合。 可以利用与该区域相关联的传感器来促进测定。 然后可以通过各种方法来表征细胞的分子谱，例如DNA分析，RNA分析和蛋白质分析。 然后，动态行为和分子谱可以用于各种目的，例如预测治疗的临床结果，筛选细胞，促进治疗，诊断疾病和监测细胞活性。

公开(公告)号：WO2017035117A1

公开(公告)日：2017-03-02

申请号：PCT/US2016/048135

申请日：2016-08-23

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： VARADARAJAN, Navin ,  ROMAIN, Gabrielle ,  COOPER, Laurence ,  SINGH, Harjeet

IPC: C12N5/0783 ,  G01N33/50 ,  A61K35/17

CPC classification number: A61K35/17 ,  C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/53 ,  C12N2501/599 ,  G01N33/502

Abstract: In some embodiments, the present disclosure pertains to a method of enhancing chimeric antigen receptor expressing T cell function. In some embodiments, the method comprises activating the chimeric antigen receptor expressing T cells. In some embodiments, the method further comprises determining the differential expression of at least one molecule on the chimeric antigen receptor T cells. In some embodiments, the method comprises targeting the at least one molecule. In some embodiments, the present disclosure pertains to a method of treating a tumor in a subject in need thereof. In some embodiments, the method comprises administering to the subject an infusion of chimeric antigen receptor expressing T cells. In some embodiments, the method further comprises determining the differential expression of at least one molecule on the chimeric antigen receptor T cells. In some embodiments, the method comprises targeting the at least one molecule, wherein the molecule is differentially expressed upon activation of the chimeric antigen receptor expressing T cells.

Abstract translation: 在一些实施方案中，本公开涉及增强表达T细胞功能的嵌合抗原受体的方法。 在一些实施方案中，该方法包括激活表达T嵌合抗原的T细胞。 在一些实施方案中，所述方法还包括测定嵌合抗原受体T细胞上至少一个分子的差异表达。 在一些实施方案中，该方法包括靶向至少一种分子。 在一些实施方案中，本公开涉及在有需要的受试者中治疗肿瘤的方法。 在一些实施方案中，所述方法包括向受试者施用表达T细胞的嵌合抗原受体的输注。 在一些实施方案中，所述方法还包括测定嵌合抗原受体T细胞上至少一个分子的差异表达。 在一些实施方案中，所述方法包括靶向所述至少一种分子，其中所述分子在表达T细胞的嵌合抗原受体活化时被差异表达。

公开(公告)号：EP3274720A1

公开(公告)日：2018-01-31

申请号：EP16769835.6

申请日：2016-03-28

Applicant: University of Houston System

Inventor： VARADARAJAN, Navin ,  ROMAIN, Gabrielle ,  LIADI, Ivan ,  SENDRA, Victor, G. ,  ROYSAM, Badrinath

IPC: G01N33/58 ,  G01N33/52 ,  C12Q1/68

CPC classification number: G01N33/56966 ,  C12Q1/68 ,  C12Q1/6869 ,  G01N33/5005 ,  G01N2570/00

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

公开(公告)号：EP4306955A2

公开(公告)日：2024-01-17

申请号：EP23212078.2

申请日：2016-03-28

Applicant: University of Houston System

Inventor： VARADARAJAN, Navin ,  ROMAIN, Gabrielle ,  LIADI, Ivan ,  SENDRA, Victor, G. ,  ROYSAM, Badrinath

IPC: G01N33/50

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.

公开(公告)号：EP4306955A3

公开(公告)日：2024-03-06

申请号：EP23212078.2

申请日：2016-03-28

Applicant: University of Houston System

Inventor： VARADARAJAN, Navin ,  ROMAIN, Gabrielle ,  LIADI, Ivan ,  SENDRA, Victor, G. ,  ROYSAM, Badrinath

IPC: G01N33/58 ,  G01N33/52 ,  C12Q1/68 ,  G01N33/50

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis. The dynamic behavior and molecular profile can then be correlated for various purposes, such as predicting clinical outcome of a treatment, screening cells, facilitating a treatment, diagnosing a disease, and monitoring cellular activity.