公开(公告)号：US12214298B2

公开(公告)日：2025-02-04

申请号：US17048779

申请日：2019-04-17

Applicant: University of Houston System

Inventor： Richard C. Willson ,  Ujwal Patil ,  Binh V. Vu ,  Ekaterini Kourentzi ,  Mary Crum

IPC: B01D15/38 ,  G01N27/00 ,  G01N30/88

Abstract: The present disclosure relates to the detection of analytes in high volumetric flow applications. Particular embodiments relate to the use of fluorescence polarization/anisotropy based for detection of analytes in a flow cell. In one testing format, an analyte of interest is probed with reagents containing fluorescent labeled recognition elements. When present in a sample or portion of a sample, the labeled analyte produces a shift in fluorescence polarization/anisotropy/intensity/lifetime as the output signal following the binding of the recognition elements to the analytes.

公开(公告)号：US11307146B2

公开(公告)日：2022-04-19

申请号：US15661696

申请日：2017-07-27

Applicant: University of Houston System ,  Instituto Tecnológico y de Estudios Superiores de Monterrey

Inventor： Richard C. Willson ,  Jinsu Kim ,  Binh V. Vu ,  Olga Patricia Vázquez Villegas ,  Federico Augusto Ruiz Ruiz ,  Marco Antonio Rito Palomares

IPC: G01N21/76 ,  C12Q1/26 ,  G01N33/62 ,  G01N33/66 ,  G01N33/70 ,  G01N21/75

Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

公开(公告)号：US20180112210A1

公开(公告)日：2018-04-26

申请号：US15571220

申请日：2016-05-23

Applicant: UNIVERSITY OF HOUSTON SYSTEM

Inventor： Navin Varadarajan ,  Jay R. Adolacion ,  Richard C. Willson

IPC: C12N15/10

CPC classification number: C12N15/1062 ,  C12N15/10 ,  C12N15/1037 ,  C12Q1/68 ,  C40B40/10 ,  G01N33/543 ,  G01N33/68 ,  G01N33/6845

Abstract: In some embodiments, the present disclosure pertains to method of screening a biological sample for a plurality of diseases. In some embodiments, such a method comprises obtaining a biological sample from a subject in need thereof. In some embodiments, the biological sample comprises a plurality of biomarkers. In some embodiments, each of the plurality of biomarkers is specific for at least one disease. In some embodiments, the method comprises contacting the biological sample with a display library of peptides. In some embodiments, each peptide in the library may have a unique amino acid sequence. In some embodiments, each of the peptides is physically linked to a nucleic acid sequence that identifies of encodes the peptide. In some embodiments, at least one of the peptides is capable of binding to at least one of the biomarkers in the biological sample. In some embodiments, the method comprises separating the bound peptide particles from the unbound peptide particle. In some embodiments, the method comprises eluting the bound peptide particles from the bound state. In some embodiments, the method comprises determining the identity of the nucleic acid sequences physically linked to the bound peptide particles. In some embodiments, the method comprises comparing the sequences thus obtained to a database of sequences representing a plurality of biomarkers of diseases.