公开(公告)号：WO1991000924A1

公开(公告)日：1991-01-24

申请号：PCT/US1990003831

申请日：1990-07-09

Applicant: UNIVERSITY OF KANSAS

Inventor： UNIVERSITY OF KANSAS ,  THOMAS, Stanley ,  TILZER, Lowell ,  MORENO, Ruben

IPC: C12Q01/68

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  Y10S435/803

Abstract: The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a silica gel polymer to the mixture of sample and separating reagent followed by agitation via, e.g., centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.

公开(公告)号：WO1997026823A1

公开(公告)日：1997-07-31

申请号：PCT/US1997001037

申请日：1997-01-21

Applicant: UNIVERSITY OF KANSAS

Inventor： UNIVERSITY OF KANSAS ,  DORFMEISTER, Josef ,  FREI, Mark ,  LERNER, David ,  OSORIO, Ivan ,  RALSTON, John

IPC: A61B05/0476

CPC classification number: A61B5/415 ,  A61B5/048 ,  A61B5/4094 ,  A61B5/7257 ,  A61B5/726 ,  A61N1/08 ,  A61N1/36025 ,  A61N1/36064 ,  A61N1/36135 ,  G06F19/00

Abstract: A system (10) analyzes signals representative of a subject's brain activity in a signal processor (12) for information indicating the subject's current activity state and for predicting a change in the activity state. One preferred embodiment uses a combination of nonlinear filtering methods to perform real-time analysis of the electro-encephalogram (EEG) or electro-corticogram (ECoG) signals from a subject patient for information indicative of or predictive of a seizure, and to complete the needed analysis at least before clinical seizure onset. The preferred system then performs an output task for prevention or abatement of the seizure, or for recording pertinent data.

Abstract translation: 系统（10）在信号处理器（12）中分析表示受试者的大脑活动的信号，用于指示受试者的当前活动状态的信息，以及用于预测活动状态的变化。 一个优选实施例使用非线性滤波方法的组合来执行来自对象患者的电脑电图（EEG）或电皮质图（ECoG）信号的实时分析，用于指示或预测癫痫发作的信息，并且完成 至少在临床癫痫发作前需要进行分析。 优选的系统然后执行输出任务以防止或减少缉获量，或用于记录相关数据。

公开(公告)号：WO1992018137A1

公开(公告)日：1992-10-29

申请号：PCT/US1992002859

申请日：1992-04-08

Applicant: UNIVERSITY OF KANSAS

Inventor： UNIVERSITY OF KANSAS ,  ANDERSON, H., Clarke

IPC: A61K35/12

CPC classification number: C07K14/51 ,  A61K35/13

Abstract: The invention relates to a method for inducing bone formation via administration of an extract of devitalized, freeze dried Saos-2 cells. The extract is prepared by contacting the freeze dried Saos-2 cells, which are devitalized in the act of freeze drying, with a weak denaturing agent of the type used to separate proteins from cells. It is not clear that the active ingredient in the extract is a protein; however, the extract does have properties superior to comparable treatment using whole Saos-2 cells. Also, described are the extracts themselves and various compositions containing it.

Abstract translation: 本发明涉及通过施用失活的冷冻干燥的Saos-2细胞的提取物诱导骨形成的方法。 通过使冻干干燥的Saos-2细胞与用于从细胞中分离蛋白质的类型的弱变性试剂接触将冷冻干燥的冻干的Saos-2细胞接触而制备提取物。 不清楚提取物中的活性成分是蛋白质; 然而，提取物确实具有优于使用整个Saos-2细胞的可比治疗的性质。 此外，描述了提取物本身和含有它的各种组合物。