公开(公告)号：WO1998000556A1

公开(公告)日：1998-01-08

申请号：PCT/US1997012425

申请日：1997-07-03

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  KIM, Jin-Seok ,  MARUYAMA, Atsushi ,  AKAIKE, Toshihiro ,  KIM, Sung, Wan

IPC: C12N15/64

CPC classification number: C12N15/1135 ,  A61K38/00 ,  A61K47/645 ,  A61K47/6917 ,  C12N15/87 ,  C12N2310/3513 ,  Y10T428/2984

Abstract: A composition for delivery of a selected nucleic acid into a targeted host cell comprises a complex of a hydrophobized, positively charged, biocompatible polymer; a lipoprotein; and a selected nucleic acid. Hydrophobic and electrostatic interactions between the components provide for the formation of the condensed DNA-containing complex. Preferred embodiments of the complex have a slightly positive surface charge and an average diameter of about 200 nm. Plasmids and oligonucleotides can be efficiently delivered with such compositions. A method of delivering a selected nucleic acid to a host cell is also disclosed.

Abstract translation: 用于将选择的核酸递送至靶宿主细胞的组合物包含疏水化，带正电荷的生物相容性聚合物的复合物; 脂蛋白 和选择的核酸。 组分之间的疏水和静电相互作用提供了形成含缩合DNA的复合物。 络合物的优选实施方案具有略微正的表面电荷和约200nm的平均直径。 可以用这样的组合物有效地递送质粒和寡核苷酸。 还公开了将选择的核酸递送至宿主细胞的方法。

公开(公告)号：EP0958369A1

公开(公告)日：1999-11-24

申请号：EP97934981.0

申请日：1997-07-03

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： KIM, Jin-Seok ,  MARUYAMA, Atsushi ,  AKAIKE, Toshihiro ,  KIM, Sung, Wan

IPC: C12N15 ,  A61K47 ,  C08L89 ,  A61K38

CPC classification number: C12N15/1135 ,  A61K38/00 ,  A61K47/645 ,  A61K47/6917 ,  C12N15/87 ,  C12N2310/3513 ,  Y10T428/2984

Abstract: A composition for delivery of a selected nucleic acid into a targeted host cell comprises a complex of a hydrophobized, positively charged, biocompatible polymer; a lipoprotein; and a selected nucleic acid. Hydrophobic and electrostatic interactions between the components provide for the formation of the condensed DNA-containing complex. Preferred embodiments of the complex have a slightly positive surface charge and an average diameter of about 200 nm. Plasmids and oligonucleotides can be efficiently delivered with such compositions. A method of delivering a selected nucleic acid to a host cell is also disclosed.