公开(公告)号：WO1997000941A3

公开(公告)日：1997-01-09

申请号：PCT/US1996011149

申请日：1996-06-24

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： UNIVERSITY OF UTAH RESEARCH FOUNDATION ,  TRUJILLO, Edward, M. ,  RAPPAPORT, Catherine

IPC: C12N11/08

公开(公告)号：EP0871700A2

公开(公告)日：1998-10-21

申请号：EP96923574.0

申请日：1996-06-24

Applicant: THE UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： TRUJILLO, Edward, M. ,  RAPPAPORT, Catherine

IPC: C12M3 ,  C12N5

CPC classification number: C12M23/20 ,  C12M25/06 ,  C12N5/0068 ,  C12N2533/20 ,  C12N2533/54

Abstract: Anchorage-dependent cells are grown on a novel substratum which is belived to increase the oxygenation of the cells and reduce the formation of free-radicals. The substratum consists of a perfluorocarbon reservoir which is coated with a layer of protein (e.g., collagen or gelatin) which has been perfluoroalkylated. The perfluoroalkylated protein forms a very stable surface to which anchorage-dependent cells can attach and grow. Using this system, mammalian cell cultures have been grown to densities exceeding 107 cells/cm2. The increased density is attributed to the formation of multiple cell layers which resemble tissue-like structures.