公开(公告)号：US10948411B2

公开(公告)日：2021-03-16

申请号：US15711102

申请日：2017-09-21

Applicant: University of Houston System

Inventor： Jakoah Brgoch ,  Erin Finley ,  Andrew Paterson ,  Richard Willson

IPC: G01N21/64 ,  C09K11/77 ,  G01N33/58 ,  G01N33/543 ,  G01N21/84

Abstract: Disclosed are methods of detecting one or more analytes in a sample by: (1) associating the sample with a surface that includes an analyte binding agent to result in the immobilization of the analytes on the surface; (2) contacting the analyte with a composition that includes at least one phosphor compound with an affinity for the analyte; (3) formation of immobilized analyte binding agent-analyte-phosphor complexes on the surface; (4) separating unbound phosphor compounds from the immobilized complexes; (5) detecting a presence or absence of a luminescence signal from the immobilized complexes; and (6) correlating the luminescence signal to the presence or absence of the analyte in the sample. The phosphor compound may include (Sr1-δBaδ)2-j-kMgSi2O7:EujDyk, (Sr1-δBaδ)2-xMgSi2O7:Eu2+Dy3+, (Sr1-δBaδ)2MgSi2O7:Eu2+Dy3+, (Sr1-δBaδ)2-xMgSi2O7:Eu2+, and combinations thereof. Additional phosphor compounds may also be utilized, such as [AE]2MgSi2O7:Eu2+, [AE]Al2O4:Eu2+, Dy3+, and combinations thereof, where AE is at least one of Ca, Sr, or Ba.