公开(公告)号：US20210178290A1

公开(公告)日：2021-06-17

申请号：US17048779

申请日：2019-04-17

Applicant: University of Houston System

Inventor： Richard C. WILLSON ,  Ujwal PATIL ,  Binh V. VU ,  Ekaterini KOURENTZI ,  Mary CRUM

IPC: B01D15/38 ,  G01N27/00

Abstract: The present disclosure relates to the detection of analytes in high volumetric flow applications. Particular embodiments relate to the use of fluorescence polarization/anisotropy based for detection of analytes in a flow cell. In one testing format, an analyte of interest is probed with reagents containing fluorescent labeled recognition elements. When present in a sample or portion of a sample, the labeled analyte produces a shift in fluorescence polarization/anisotropy/intensity/lifetime as the output signal following the binding of the recognition elements to the analytes.

公开(公告)号：US20250128189A1

公开(公告)日：2025-04-24

申请号：US19005828

申请日：2024-12-30

Applicant: University of Houston System

Inventor： Richard C. WILLSON ,  Ujwal PATIL ,  Binh V. VU ,  Ekaterini KOURENTZI ,  Mary CRUM

IPC: B01D15/38 ,  G01N27/00 ,  G01N30/88

Abstract: The present disclosure relates to the detection of analytes in high volumetric flow applications. Particular embodiments relate to the use of fluorescence polarization/anisotropy based for detection of analytes in a flow cell. In one testing format, an analyte of interest is probed with reagents containing fluorescent labeled recognition elements. When present in a sample or portion of a sample, thee labeled analyte produces a shift in fluorescence polarization/anisotropy/intensity/lifetime as the output signal following the binding of the recognition elements to the analytes.

公开(公告)号：US20180031484A1

公开(公告)日：2018-02-01

申请号：US15661696

申请日：2017-07-27

Applicant: University of Houston System ,  Tecnologico de Monterrey

Inventor： Richard C. WILLSON ,  Jinsu KIM ,  Binh V. VU ,  Olga Patricia VÁZQUEZ VILLEGAS ,  Federico Augusto RUIZ RUIZ ,  Marco Antonio RITO PALOMARES

IPC: G01N21/76 ,  G01N33/62 ,  G01N33/70 ,  C12Q1/26 ,  G01N33/66

CPC classification number: G01N21/76 ,  C12Q1/26 ,  G01N33/62 ,  G01N33/66 ,  G01N33/70 ,  G01N2021/755 ,  G01N2800/042

Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

公开(公告)号：US20220221408A1

公开(公告)日：2022-07-14

申请号：US17710946

申请日：2022-03-31

Applicant: University of Houston System ,  Instituto Tecnológico y de Estudios Superiores de Monterrey

Inventor： Richard C. WILLSON ,  Jinsu KIM ,  Binh V. VU ,  Olga Patricia VÁZQUEZ VILLEGAS ,  Federico Augusto RUIZ RUIZ ,  Marco Antonio RITO PALOMARES

IPC: G01N21/76 ,  C12Q1/26 ,  G01N33/62 ,  G01N33/66 ,  G01N33/70

Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

公开(公告)号：US20220011315A1

公开(公告)日：2022-01-13

申请号：US17291768

申请日：2019-11-06

Applicant: University of Houston System

Inventor： Binh V. VU ,  Richard C. WILLSON

IPC: G01N33/58 ,  G01N33/543 ,  G01N21/76

Abstract: The present disclosure relates to improved light-based assays, especially ligand binding assays, in which light is emitted from moieties stimulated by stimulating moieties. There is provided a method of generating and detecting a light signal comprising (a) providing a fluid sample comprising an analyte of interest; (b) contacting said sample with a first reagent comprising a molecular recognition element that binds to said analyte; (c) contacting said sample with a second reagent that (i) binds to said analyte in the presence of said first reagent; or (ii) binds to said first reagent; wherein said second reagent contains a light-generating reporter or chemiexcitation emitter; (d) removing unbound first and second reagents; (e) introducing into said sample a source of singlet oxygen or a chemiexcitation stimulator; and (f) measuring the production of light from said reporter.