公开(公告)号：US20240279614A1

公开(公告)日：2024-08-22

申请号：US18652960

申请日：2024-05-02

Applicant: University of Science and Technology of China

Inventor： Gang ZHAO ,  Conghui TIAN ,  Lingxiao SHEN

IPC: C12N5/071 ,  A01N1/02

CPC classification number: C12N5/0682 ,  A01N1/0221 ,  A01N1/0231 ,  A01N1/0284 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/235 ,  C12N2501/31 ,  C12N2529/00 ,  C12N2531/00

Abstract: Methods for cryopreservation and resuscitation of follicles are provided by the present disclosure, relating to the technical field of follicular cryopreservation. According to the method for cryopreserving follicles provided by the present disclosure, follicles are firstly encapsulated with Alginate hydrogel, and incubated in protectant A and B. and moved into a protective solution A for incubation. Follicles encapsulated in hydrogel microcapsules are then loaded into straws and immediately submerged into liquid nitrogen for cryopreservation. Then, they are rapidly warmed by a 37° C. water bath combined with nano-warming technique. To verify the preservation effect, the warmed preantral follicles are further cultured in vitro in three dimensions.