公开(公告)号：JP2012110327A

公开(公告)日：2012-06-14

申请号：JP2011274389

申请日：2011-12-15

Applicant: VERTEX PHARMA SAN DIEGO LLC

Inventor： MAHER MICHAEL P ,  GONZALES JESUS E III

IPC: C12Q1/02 ,  G01N33/15 ,  G01N33/50

Abstract: PROBLEM TO BE SOLVED: To provide reliable and specific methods, with existing need therefor, for regulating the membrane potentials of living cells that are compatible with optical methods of analysis and are readily amendable to high throughput analysis.SOLUTION: A method for characterizing the biological activity of a candidate compound includes exposing cells to the candidate compound, and then exposing the cells to a repetitive application of electric fields to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.

公开(公告)号：WO2005022113A3

公开(公告)日：2005-05-19

申请号：PCT/US2004022047

申请日：2004-07-08

Applicant: VERTEX PHARMA ,  MAHER MICHAEL P ,  GONZALEZ III JESUS E

Inventor： MAHER MICHAEL P ,  GONZALEZ III JESUS E

IPC: G01N33/487 ,  G01N33/50 ,  G01N33/68 ,  C12N15/00 ,  C12N15/63 ,  G01N25/18 ,  G01N33/566

CPC classification number: G01N33/5038 ,  G01N33/48728 ,  G01N33/5008 ,  G01N33/5076 ,  G01N33/5088 ,  G01N33/542 ,  G01N33/6872

Abstract: A method of characterizing the biological activity of a candidate compound may include exposing cells to the candidate compound, and then exposing the cells to a repetitive application of electric fields so as to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.

Abstract translation: 表征候选化合物的生物活性的方法可以包括将细胞暴露于候选化合物，然后将细胞暴露于电场的重复施用，以将跨膜电位设定为与预选电压相关的水平 目标离子通道的状态。

公开(公告)号：EP1644497A4

公开(公告)日：2007-08-08

申请号：EP04801966

申请日：2004-07-08

Applicant: VERTEX PHARMA

Inventor： MAHER MICHAEL P ,  GONZALEZ III JESUS E

IPC: C12N15/00 ,  C12N15/63 ,  G01N25/18 ,  G01N33/487 ,  G01N33/50 ,  G01N33/566 ,  G01N33/68

CPC classification number: G01N33/5038 ,  G01N33/48728 ,  G01N33/5008 ,  G01N33/5076 ,  G01N33/5088 ,  G01N33/542 ,  G01N33/6872

Abstract: A method of characterizing the biological activity of a candidate compound may include exposing cells to the candidate compound, and then exposing the cells to a repetitive application of electric fields so as to set the transmembrane potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.

公开(公告)号：DK1303757T3

公开(公告)日：2007-02-05

申请号：DK01953433

申请日：2001-07-09

Applicant: VERTEX PHARMA SAN DIEGO LLC

Inventor： GONZALEZ JESUS E III ,  MAHER MICHAEL P

IPC: G01N21/64 ,  G01N33/483 ,  C12Q1/02 ,  G01N21/78 ,  G01N27/416 ,  G01N33/15 ,  G01N33/50 ,  G01N33/68 ,  G01N37/00

公开(公告)号：AU2006202851B2

公开(公告)日：2009-06-04

申请号：AU2006202851

申请日：2006-07-04

Applicant: VERTEX PHARMA SAN DIEGO LLC

Inventor： GONZALEZ JESUS E III ,  MAHER MICHAEL P

IPC: G01N21/64 ,  G01N33/00 ,  C12Q1/02 ,  G01N21/78 ,  G01N27/416 ,  G01N33/15 ,  G01N33/50 ,  G01N33/68 ,  G01N37/00

公开(公告)号：AU2006202851A1

公开(公告)日：2006-07-27

申请号：AU2006202851

申请日：2006-07-04

Applicant: VERTEX PHARMA SAN DIEGO LLC

Inventor： GONZALEZ JESUS E III ,  MAHER MICHAEL P

IPC: G01N21/64 ,  G01N33/00 ,  C12Q1/02 ,  G01N21/78 ,  G01N27/416 ,  G01N33/15 ,  G01N33/50 ,  G01N33/68 ,  G01N37/00

公开(公告)号：CA2532973A1

公开(公告)日：2005-03-10

申请号：CA2532973

申请日：2004-07-08

Applicant: VERTEX PHARMA

Inventor： MAHER MICHAEL P ,  GONZALEZ JESUS E III

IPC: C12N15/00 ,  C12N15/63 ,  G01N25/18 ,  G01N33/487 ,  G01N33/50 ,  G01N33/566 ,  G01N33/68

Abstract: A method of characterizing the biological activity of a candidate compound m ay include exposing cells to the candidate compound, and then exposing the cell s to a repetitive application of electric fields so as to set the transmembran e potential to a level corresponding to a pre-selected voltage dependent state of a target ion channel.

公开(公告)号：CA2763047C

公开(公告)日：2013-08-27

申请号：CA2763047

申请日：2001-07-09

Applicant: VERTEX PHARMA SAN DIEGO LLC

Inventor： MAHER MICHAEL P ,  GONZALEZ JESUS E III

IPC: C12M1/42 ,  C12M1/34 ,  C12Q1/02 ,  C40B60/12 ,  G01N21/03 ,  G01N21/15 ,  G01N33/483 ,  G01N33/50 ,  G01N33/68 ,  G01N35/00

Abstract: A high throughput screening device may comprise high-transmittance wells, electrodes disposed in the wells, and optical detection means. The device may be configured to apply an electric field in the wells to alter the transmembrane potential of cells disposed in the wells and produce detectable light emissions corresponding to ion channel activity.

公开(公告)号：AU2010246565A1

公开(公告)日：2010-12-23

申请号：AU2010246565

申请日：2010-12-01

Applicant: VERTEX PHARMA

Inventor： MAHER MICHAEL P ,  GONZALEZ JESUS E III

IPC: G01N33/487 ,  G01N33/50 ,  G01N33/68

公开(公告)号：AT480771T

公开(公告)日：2010-09-15

申请号：AT06019986

申请日：2001-07-09

Applicant: VERTEX PHARMA SAN DIEGO LLC

Inventor： MAHER MICHAEL P ,  GONZALES JESUS E III

IPC: G01N21/64 ,  G01N33/483 ,  C12Q1/02 ,  G01N21/78 ,  G01N27/416 ,  G01N33/15 ,  G01N33/50 ,  G01N33/68 ,  G01N37/00