公开(公告)号：WO2005059099A2

公开(公告)日：2005-06-30

申请号：PCT/US2004/041619

申请日：2004-12-13

Applicant: WASHINGTON UNIVERSITY IN ST.LOUIS ,  GROSS, Richard ,  JENKINS, Christopher, M.

Inventor： GROSS, Richard ,  JENKINS, Christopher, M.

IPC: C12N

CPC classification number: C12N9/20 ,  A61K38/00

Abstract: A novel function phospholipase A2, referred to herein as calcium­independent phospholipase A 2 ? (iPLA 2 ?) having SEQ ID NO: 1 and SEQ ID NO: 2, and nucleic acid sequences (SEQ ID NO: 3 and SEQ ID NO: 4) encoding and expressing iPLA 2 ?. This novel enzyme has been isolated and characterized and is involved in the catalysis and hydrolysis of lipids cycling in a living cell biosystem. In an embodiment, the compromises to an isolated nucleic acid molecule comprising a set of iPLA 2 ? polynucleotides. In an aspect of this embodiment, the iPLA 2 ? polynucleotides encode and express an iPLA 2 ? polypeptide. In one aspect, an isolated and characterized gene comprises a polynucleotide having a sequence shown in SEQ ID NO: 3 and SEQ ID NO: 4.

Abstract translation: 新型功能性磷脂酶A2，本文称为具有SEQ ID NO：1和SEQ ID NO：2的钙依赖性磷脂酶A2（IPLA2β）和核酸序列（SEQ ID NO：3和SEQ ID NO：4），其编码和 表达iPLA2？ 这种新型酶已经被分离和表征，并参与活细胞生物系统中脂质循环的催化和水解。 在一个实施方案中，对分离的核酸分子的折衷包括一组iPLA2？ 多核苷酸。 在本实施例的一个方面，iPLA2？ 多核苷酸编码并表达iPLA2？ 多肽。 一方面，分离和表征的基因包含具有SEQ ID NO：3和SEQ ID NO：4所示序列的多核苷酸。

公开(公告)号：WO2005067912A1

公开(公告)日：2005-07-28

申请号：PCT/US2005/000045

申请日：2005-01-03

Applicant: WASHINGTON UNIVERSITY IN ST. LOUIS ,  GROSS, Richard

Inventor： GROSS, Richard

IPC: A61K31/20

CPC classification number: A61K38/465 ,  A61K31/20 ,  C12Q1/26 ,  C12Q1/44 ,  G01N33/92 ,  G01N2333/916 ,  G01N2500/00 ,  G01N2800/044

Abstract: A method for intentionally modulating by externally directing the amount of fat in a living mammal having adipocyte cells which comprises administering a compound thereto having reactive selectivity to the metabolic regulatory system of the cells of the animal to increase the fatty acid a oxidation of the lipid content in the differentiating adipocyte thereby reducing the amount of equivalents available for ATP synthesis.

Abstract translation: 通过外部引导具有脂肪细胞的活的哺乳动物中的脂肪量来有意调节的方法，其包括向所述动物的细胞的代谢调节系统施用具有反应选择性的化合物以增加所述脂肪酸的脂质含量的氧化 在分化的脂肪细胞中，从而减少可用于ATP合成的当量。

公开(公告)号：WO2005062995A3

公开(公告)日：2005-07-14

申请号：PCT/US2004/044069

申请日：2004-12-23

Applicant: WASHINGTON UNIVERSITY IN ST. LOUIS ,  GROSS, Richard

Inventor： GROSS, Richard

IPC: C07H21/04

Abstract: A method for identifying an agonist exhibiting molecular or pharmacologic inhibition which is effective against the activity of at least one of iPLA 2 β and iPLA 2 γ which comprises culturing 3T3-L1 cells and transfecting them with negative control siRNA, siRNA directed against iPLA 2 β or siring directed against iPLA 2 γ prior to induction or during to differentiation or pharmacologic inhibition and observing for whether that down regulation of iPLA 2 β or iPLA 2 γ inhibits adipocyte differentiation.

公开(公告)号：WO1994025623A1

公开(公告)日：1994-11-10

申请号：PCT/US1994004468

申请日：1994-04-22

Applicant: WASHINGTON UNIVERSITY

Inventor： WASHINGTON UNIVERSITY ,  GROSS, Richard

IPC: C12Q01/34

CPC classification number: C12Q1/44 ,  C12N9/12 ,  C12N9/20 ,  C12Q1/48 ,  G01N2333/918 ,  Y10S435/968 ,  Y10S436/804 ,  Y10T436/12

Abstract: A method of identifying compounds that modulate the activity of myocardial calcium-independent phospholipase A2 is disclosed. In a test assay of the method of the invention, myocardial calcium-independent phospholipase A2 40kDa catalytic subunit, 85kDa phosphofructokinase isoform, ATP, a substrate and a test compound are combined and the myocardial calcium-independent phospholipase A2 activity is determined. The level of activity observed in the test assay is compared to the level of activity generated from a control assay which is similar to the test assay but which does not include the test compound. Essentially pure myocardial calcium-independent phospholipase A2 85kDa phosphofructokinase isoform is also disclosed.

Abstract translation: 公开了鉴定调节心肌钙依赖性磷脂酶A2活性的化合物的方法。 在本发明方法的测试测定中，组合心肌钙依赖性磷脂酶A240kDa催化亚基，85kDa磷酸果糖激酶同种型，ATP，底物和测试化合物，并测定心肌钙依赖性磷脂酶A2活性。 将在测试测定中观察到的活性水平与从对照测定产生的活性水平进行比较，其与测试测定类似，但不包括测试化合物。 还公开了本质上纯的心肌钙依赖性磷脂酶A285kDa磷酸果糖激酶同种型。