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    Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
    3.
    发明专利
    Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides 未知

    公开(公告)号：GB2555553A

    公开(公告)日：2018-05-02

    申请号：GB201801574

    申请日：2014-12-22

    Applicant: WATERS TECHNOLOGIES CORP

    Inventor： KEVIN WYNDHAM ,  PAMELA C IRANETA ,  STEPHEN KOZA ,  MATTHEW A LAUBER

    IPC: B01J20/289 ,  B01D15/30 ,  B01J20/26 ,  B01J20/32 ,  C08L51/08

    Abstract: A porous material comprising a co-polymer comprising at least one hydrophilic monomer and a poly-amide bonded phase, wherein the porous material comprises a polymerization product of a first monomer and a second monomer, wherein the second monomer is selected from N,N-methylenebisacrylamide, N,N-ethylenebisacrylamide, N,N-propylenebisacrylamide, N,N-butylenebisacrylamide, N,N-(1,2-dihydroxyethylene) bisacrylamide and 1,4-bis(acryloyl)piperazine. The porous material may comprise porous particles or a porous monolith comprising said copolymer. The porous material may comprise a second poly-amide bonded phase. The material preferably has an average pore diameter of at least 200 Å and a median pore diameter between 100 Å and 1000 Å; and a nitrogen content from 0.5% to 20%, preferably from 1% to 4%. Methods using said porous material for isolating, analysing and determining levels of a component from a mixture are disclosed, particularly via hydrophilic interaction liquid chromatography (HILIC). Methods are directed towards the separation of proteins and glycosylated proteins, the separation of which is disclosed to benefit from improved pore diffusion arising from increased average pore size. The porous material may be used in a separation device, such as chromatographic columns, cartridges, and thin layer chromatographic plates.

    Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
    7.
    发明专利
    Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides 未知

    公开(公告)号：GB2558103A

    公开(公告)日：2018-07-04

    申请号：GB201804593

    申请日：2014-12-22

    Applicant: WATERS TECHNOLOGIES CORP

    Inventor： KEVIN DANIEL WYNDHAM ,  STEPHEN KOZA ,  PAMELA C IRANETA ,  MATTHEW A LAUBER

    IPC: B01D15/30 ,  B01J20/26 ,  B01J20/289 ,  B01J20/32 ,  C08L51/08

    Abstract: A method of performing hydrophilic interaction liquid chromatography (HILIC) for characterizing a glycosylated proteinaceous sample comprising the steps of; preparing a sample containing the glycosylated proteinaceous sample; providing a HILIC column having an inlet and an outlet, and a stationary phase material; loading the sample on said stationary phase material at a column inlet pressure of no less than 3000 psi and flowing the sample with a mobile phase eluent through the column; and separating the sample from the outlet into one or more fractions. The glycosylated proteinaceous material is preferably a glycosylated monoclonal antibody. The stationary phase preferably comprises a porous material comprising a copolymer comprising at least one hydrophilic monomer and a poly-amide bonded phase. The poly-amide bonded phase is preferably formed of acrylamide monomers. The porous material is preferably fully porous or superficially porous, and preferably comprises pores having an average pore size of at least 200 Ǻ. The stationary phase material preferably comprises an organic-inorganic hybrid core comprising an aliphatic bridge silane. The method preferably further includes various sample preparation steps, including the addition of a diluent, the diluent preferably comprising a denaturant.

    Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides
    8.
    发明专利
    Materials for hydrophilic interaction chromatography and processes for preparation and use thereof for analysis of glycoproteins and glycopeptides 未知

    公开(公告)号：GB2535658A

    公开(公告)日：2016-08-24

    申请号：GB201605042

    申请日：2014-12-22

    Applicant: WATERS TECHNOLOGIES CORP

    Inventor： MATTHEW A LAUBER ,  STEPHEN KOZA ,  PAMELA C IRANETA ,  KEVIN WYNDHAM

    IPC: B01J20/286 ,  B01D15/30 ,  B01J20/32 ,  C08F230/08 ,  C08L33/14

    Abstract: A porous material comprising a copolymer comprising at least on hydrophilic monomer and at least a first and second poly-amide bonded phase, wherein the average pore size is greater than or equal to 200 Å or between 1 and 50Å. The porous material may comprise porous particles or a porous monolith comprising said copolymer. The hydrophilic polymer preferably comprises monomers having di or tri alkoxy silanes. The poly-amide bonded phase may comprise acrylamides, acrylates, acrylonitriles, and preferably poly(divinyl benzene-co-N-vinylcaprolactam). The material can have a median pore diameter of about 100 Å to about 1000 Å and a nitrogen content from about 0.5% to about 20%. Methods using said porous material for isolating, analysing and determining levels of a component from a mixture are disclosed, particularly via hydrophilic interaction liquid chromatography (HILIC). Methods are directed towards the separation of proteins and glycosylated proteins, due to improved pore diffusion arising from increased or decreased average pore size. The porous material may be used in a separation device, such as chromatographic columns, cartridges, and thin layer chromatographic plates.

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    9.
    发明专利
    "HIGH PURITY CHROMATROGRAPHIC MATERIALS COMPRISING AN IONIZABLE MODIFIER" 未知

    公开(公告)号：IN363DEN2012A

    公开(公告)日：2015-08-21

    申请号：IN363DEN2012

    申请日：2012-01-13

    Applicant: WATERS TECHNOLOGIES CORP

    Inventor： KEVIN D WYNDHAM ,  PAMELA C IRANETA ,  THOMAS H WALTER

    IPC: C08J9/00

    Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

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