公开(公告)号：WO2017106512A1

公开(公告)日：2017-06-22

申请号：PCT/US2016/066950

申请日：2016-12-15

Applicant: WORCESTER POLYTECHNIC INSTITUTE

Inventor： PETERSON, Amy ,  ROLLE, Marsha ,  DING, Ivan

IPC: A61K38/43 ,  A61K38/02 ,  A61P43/00

CPC classification number: C12N13/00 ,  C12M21/08 ,  C12M23/20 ,  C12M25/14 ,  C12N5/0068

Abstract: Device, and methods of using or making the device, for engineering cells in vitro are disclosed. In some aspects, a cell culture device comprises at least one glass or polymer surface configured for incubating cells in a culture medium; a charged molecule electrostatically bound to the surface; and a polyelectrolyte multilayer (PEM) electrostatically bound to the charged molecule, the PEM comprising one or more bi-layers of oppositely charged polyelectrolytes, and the PEM having a sufficient thickness to permit release of the charged molecule into the culture medium in a controlled released manner.

Abstract translation: 公开了装置以及使用或制造该装置以在体外工程化细胞的方法。 在一些方面，细胞培养装置包含至少一个配置用于在培养基中培养细胞的玻璃或聚合物表面; 带电分子静电结合到表面; 和与带电分子静电结合的聚电解质多层（PEM），所述PEM包含一个或多个带相反电荷的聚电解质的双层，并且所述PEM具有足够的厚度以允许带电分子以受控释放释放到培养基中 方式。