公开(公告)号：EP0485970A2

公开(公告)日：1992-05-20

申请号：EP91119328.2

申请日：1991-11-13

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY LIMITED

Inventor： Galili,Gad ,  Shaul,Orit ,  Perl,Avihai

IPC: C12N15/82 ,  C12N15/62 ,  C12N15/54 ,  C12N15/60 ,  A01H1/00

CPC classification number: C12N15/8254 ,  C12N9/12 ,  C12N9/88 ,  C12N15/8251 ,  C12N15/8253 ,  C12P13/08

Abstract: A chimeric gene construct comprising a DNA sequence encoding an enzyme having aspartate kinase (AK) activity is provided, which is capable of expression in plant cells with subsequent increased production Of threonine. Transgenic plants containing in their cells said chimeric gene overproduce threonine and transgenic plants containing in their cells said chimeric gene and a second chimeric gene comprising a DNA sequence coding for an enzyme having dihydrodipicolinate synthase (DHPS) activity, overproduce both threonine and lysine. The transgenic plants are resistant to lysine and threonine, to derivative: thereof and to selective inhibitors Of the plant enzymes DHFS or AK, and thus these compounds may be used as selective herbicides in locus where the transgenic plants are cultivated.

Abstract translation: 提供了包含编码具有天冬氨酸激酶（AK）活性的酶的DNA序列的嵌合基因构建体，其能够在随后增加的苏氨酸的产生中在植物细胞中表达。 在其细胞中含有转基因植物，所述嵌合基因过量产生苏氨酸和在其细胞中含有所述嵌合基因的转基因植物，以及包含编码具有二氢吡啶二羧酸合成酶（DHPS）活性的酶的DNA序列的第二嵌合基因，其过量产生苏氨酸和赖氨酸。 转基因植物对赖氨酸和苏氨酸，其衍生物和植物酶DHFS或AK的选择性抑制剂具有抗性，因此这些化合物可用作培养转基因植物的基因座中的选择性除草剂。

公开(公告)号：EP0485970A3

公开(公告)日：1992-07-01

申请号：EP91119328.2

申请日：1991-11-13

Applicant: YEDA RESEARCH AND DEVELOPMENT COMPANY LIMITED

Inventor： Galili,Gad ,  Shaul,Orit ,  Perl,Avihai

IPC: C12N15/82 ,  C12N15/62 ,  C12N15/54 ,  C12N15/60 ,  A01H1/00

CPC classification number: C12N15/8254 ,  C12N9/12 ,  C12N9/88 ,  C12N15/8251 ,  C12N15/8253 ,  C12P13/08

Abstract: A chimeric gene construct comprising a DNA sequence encoding an enzyme having aspartate kinase (AK) activity is provided, which is capable of expression in plant cells with subsequent increased production Of threonine. Transgenic plants containing in their cells said chimeric gene overproduce threonine and transgenic plants containing in their cells said chimeric gene and a second chimeric gene comprising a DNA sequence coding for an enzyme having dihydrodipicolinate synthase (DHPS) activity, overproduce both threonine and lysine. The transgenic plants are resistant to lysine and threonine, to derivative: thereof and to selective inhibitors Of the plant enzymes DHFS or AK, and thus these compounds may be used as selective herbicides in locus where the transgenic plants are cultivated.