公开(公告)号：KR1020050028700A

公开(公告)日：2005-03-23

申请号：KR1020030065212

申请日：2003-09-19

Applicant: 건국대학교 산학협력단

Inventor： 안중훈 ,  김대환 ,  김봉규 ,  임융호

IPC: C12N9/10

CPC classification number: C12N9/1007 ,  C12Y201/01232

Abstract: Naringenin 7-O-methyltransferase and a method for preparing Sakuranetin using a gene encoding the same enzyme are provided, thereby reducing the preparation costs of Sakuranetin by biological preparation, and improving disease tolerance of plants by inserting the Sakuranetin into the plants. The Naringenin 7-O-methyltransferase converts Naringenin into Sakuranetin having the amino acid sequence set forth in SEQ ID NO:1. A gene encoding the Sakuranetin has the nucleotide sequence set forth in SEQ ID NO:2, wherein the Naringenin 7-O-methyltransferase gene is isolated from bean Glycine max. The method for preparing Sakuranetin using a gene encoding the same enzyme comprises the steps of: constructing an expression vector containing the Naringenin 7-O-methyltransferase gene; transforming E. coli with the expression vector; and culturing the transformed E. coli.

Abstract translation: 提供柚皮素7-O-甲基转移酶和使用编码相同酶的基因制备樱草素的方法，从而通过生物制备降低樱草素的制备成本，并通过将樱花素插入植物来提高植物的耐病性。 柚皮素7-O-甲基转移酶将柚皮素转化为具有SEQ ID NO：1所示氨基酸序列的樱花菜素。 编码樱花素的基因具有SEQ ID NO：2所示的核苷酸序列，其中，从豆荚豆中分离出柚皮素7-O-甲基转移酶基因。 使用编码相同酶的基因制备樱草素的方法包括以下步骤：构建含有柚皮苷7-O-甲基转移酶基因的表达载体; 用表达载体转化大肠杆菌; 并培养转化的大肠杆菌。