公开(公告)号：US12275697B2

公开(公告)日：2025-04-15

申请号：US17605769

申请日：2020-04-23

Applicant: Stellenbosch University

Inventor： Ndumiso Sibanda ,  Harold Pasch ,  Helen Pfukwa

IPC: C07C45/59 ,  C07C27/00 ,  C07C37/54 ,  C07C41/01 ,  C07C45/67 ,  C07G1/00 ,  C08J11/28 ,  C08L97/00 ,  C10G1/00

Abstract: The invention provides a method for depolymerising a phenolic polymer, the method comprising reacting the phenolic polymer with dimethylsulphoxide (DMSO) and a hydrogen halide. The phenolic polymer may be selected from the group consisting of lignin and derivatives thereof. The hydrogen halide may be HBr. The quantity of hydrogen halide per gram of phenolic polymer may be from 30 mmoles to 70 mmoles. The quantity of DMSO per gram of phenolic polymer may be from 0.1 mole to 1 mole. The reaction may be performed at a temperature of from 100 to 120° C. The reaction may be carried out for between 10 h and 14 h. The product of the reaction may comprise vanillin.

公开(公告)号：US20240382971A1

公开(公告)日：2024-11-21

申请号：US18691997

申请日：2022-09-13

Applicant: STELLENBOSCH UNIVERSITY ,  SOUTH AFRICAN MEDICAL RESEARCH COUNCIL

Inventor： Stephan DIJKSTRA ,  Grant De Vos THERON ,  Martinus Johannes NIEUWOUDT ,  Rouxjeane VENTER ,  Robin Mark WARREN

IPC: B01L99/00

Abstract: An extraction device assembly for extracting a sample from a sample-containing chamber of a nucleic acid amplification cartridge is provided. The assembly has a cartridge interface forming a receptacle configured to at least partially fit the sample-containing chamber therein. The cartridge interface has an aperture configured to at least partially expose the sample-containing chamber, and a collection interface having an aperture configured to at least partially align with the cartridge interface aperture and with the sample-containing chamber positioned at least partially between them. The collection interface aperture is in fluid communication with a collector. The extraction device assembly is operable to fit the sample-containing chamber in the receptacle of the cartridge interface and guide a piercing tool into the cartridge interface aperture to pierce the sample-containing chamber and liberate a sample contained therein for transferral to the collector.

公开(公告)号：US11944102B2

公开(公告)日：2024-04-02

申请号：US15733580

申请日：2019-03-05

Applicant: Stellenbosch University

Inventor： Anna-Maria Botha-Oberholster ,  Hendrik Willem Swiegers ,  Nicolaas Francois Visser Burger

IPC: A01N63/60 ,  C12N15/113

CPC classification number: A01N63/60 ,  C12N15/113

Abstract: The invention provides siRNA molecules for use in controlling pest infestation. The siRNA molecules target the mature mRNA of D. noxia cprr1-8 in a region between nucleotides 464 and 774 of SEQ ID NO: 23, or an equivalent region of an ortholog of D. noxia cprr1-8. Ingestion of the siRNA molecule by a pest inhibits the biological activity of the pest. In one embodiment, the siRNA molecule comprises a polynucleotide which has at least 80% sequence identity to the sequence 5′ UAAACAAUCGCAAGAAGCUGA 3′ (SEQ ID NO: 1) and a polynucleotide which has at least 80% sequence identity to the sequence 5′ AGCUUCUUGCGAUUGUUUAAG 3′ (SEQ ID NO: 2). Compositions comprising the siRNA molecules, vectors encoding the siRNA molecules, and methods for using the siRNA molecules are also provided.

公开(公告)号：US20240026166A1

公开(公告)日：2024-01-25

申请号：US18028396

申请日：2021-09-17

Applicant: Stellenbosch University

Inventor： Wesaal Khan ,  Thando Ndlovu ,  Tanya Lee Clements ,  Nusrat Begum Mutta

IPC: C09D5/16 ,  A01N25/10 ,  A01N63/22 ,  A01N63/27 ,  A01N25/24 ,  A01P1/00 ,  A01N25/30

CPC classification number: C09D5/1625 ,  A01N25/10 ,  A01N63/22 ,  A01N63/27 ,  A01N25/24 ,  A01P1/00 ,  A01N25/30

Abstract: The invention provides a coating method for coating a surface of a substrate material with a biosurfactant. The method includes the following steps: modifying the biosurfactant to promote its reactivity with a silane linker; oxidising the surface of the substrate material; functionalising the surface of the substrate material with a silane linker; and reacting the modified biosurfactant with the functionalised surface. The biosurfactant becomes covalently bonded to the surface of the substrate material. The substrate material may be a polymer such as high-density polyethylene or polyvinyl chloride, or a ferrous metal such as stainless steel. The biosurfactant may be produced by one or more bacterial strains selected from the group consisting of Pseudomonas aeruginosa, Bacillus amyloliquefaciens and Serratia marcescens. The invention also provides articles of manufacture which include substrate materials that are at least partially coated with biosurfactants. The substrate materials and biosurfactants may be as described above.

公开(公告)号：US11833179B2

公开(公告)日：2023-12-05

申请号：US16755801

申请日：2018-10-15

Applicant: Stellenbosch University

Inventor： Deon Pieter Neveling ,  Leon Milner Theodore Dicks

IPC: A61K35/747 ,  A23K50/75 ,  A23K10/18 ,  A61P31/04 ,  A61K9/00 ,  A61K35/742 ,  A61K35/744

CPC classification number: A61K35/747 ,  A23K10/18 ,  A23K50/75 ,  A61K9/0056 ,  A61K35/742 ,  A61K35/744 ,  A61P31/04 ,  A23Y2220/43 ,  A23Y2220/79

Abstract: A probiotic composition comprising Bacillus amyloliquefaciens, Enterococcus faecalis, Lactobacillus salivarius, Lactobacillus johnsonii, Lactobacillus gallinarum and Lactobacillus crispatus is provided. The probiotic composition stimulates the immune response of chickens without negatively affecting growth performance. The probiotic composition can be added to poultry feed to improve the health and performance of chickens, and can be used as a replacement for antibiotic supplementation.

公开(公告)号：US11613782B2

公开(公告)日：2023-03-28

申请号：US16980121

申请日：2019-03-13

Applicant: UNIVERSITY OF CAPE TOWN ,  STELLENBOSCH UNIVERSITY ,  SEATTLE CHILDREN'S HOSPITAL ,  MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V ,  UNITED KINGDOM RESEARCH AND INNOVATION

Inventor： Sara Suliman ,  Ethan Greene Thompson ,  Jayne Suzanne Sutherland ,  Stefan H. E. Kaufmann ,  Thomas Jens Scriba ,  Daniel Edward Zak ,  Gerhard Walzl

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6883

Abstract: The invention provides a gene signature for use in determining a likelihood of a latent tuberculosis (TB) infection in a subject transitioning to active TB disease. The gene signature comprises at least SEPT4 and BLK, and optionally also GAS6 and/or CD1C. Expression levels of these genes are detected in a sample from the subject, and the ratios of expression of at least two of the above genes are calculated (e.g. SEPT4:BLK, SEPT4:CD1C, GAS6:BLK and/or GAS6:CD1C). A score is assigned to each ratio, the score being indicative of the likelihood of the latent TB infection transitioning into active TB disease, based on the ratio for the respective gene pair. The subject can be identified as having a latent TB infection that is likely to transition into active TB disease or that is not likely to transition into active TB disease based on the score or on the average of the scores.

公开(公告)号：US11193130B2

公开(公告)日：2021-12-07

申请号：US16426563

申请日：2019-05-30

Applicant: Lallemand Hungary Liquidity Management LLC ,  Stellenbosch University

Inventor： Elena Brevnova ,  John E. McBride ,  Erin Wiswall ,  Kevin S. Wenger ,  Nicky Caiazza ,  Heidi Hau ,  Aaron Argyros ,  Frank Agbogbo ,  Charles F. Rice ,  Trisha Barrett ,  John S. Bardsley ,  Abigail Foster ,  Anne K. Warner ,  Mark Mellon ,  Ryan Skinner ,  Indraneel Shikhare ,  Riaan Den Haan ,  Chhayal V. Gandhi ,  Alan Belcher ,  Vineet B. Rajgarhia ,  Allan C. Froehlich ,  Kristen M. Deleault ,  Emily Stonehouse ,  Shital A. Tripathi ,  Jennifer Gosselin ,  Yin-Ying Chiu ,  Haowen Xu

IPC: C12N15/81 ,  C12N9/42 ,  C12N9/24 ,  C12N15/52 ,  C12P7/06

Abstract: The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

公开(公告)号：US20210363580A1

公开(公告)日：2021-11-25

申请号：US16980121

申请日：2019-03-13

Applicant: UNIVERSITY OF CAPE TOWN ,  STELLENBOSCH UNIVERSITY ,  SEATTLE CHILDREN'S HOSPITAL DOING BUSINESS AS SEATTLE CHILDREN'S RESEARCH INSTITUTE ,  MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V ,  UNITED KINGDOM RESEARCH AND INNOVATION

Inventor： Sara Suliman ,  Ethan Greene Thompson ,  Jayne Suzanne Sutherland ,  Stefan H.E. Kaufmann ,  Thomas Jens Scriba ,  Daniel Edward Zak ,  Gerhard Walzl

IPC: C12Q1/6883

Abstract: The invention provides a gene signature for use in determining a likelihood of a latent tuberculosis (TB) infection in a subject transitioning to active TB disease. The gene signature comprises at least SEPT4 and BLK, and optionally also GAS6 and/or CD1C. Expression levels of these genes are detected in a sample from the subject, and the ratios of expression of at least two of the above genes are calculated (e.g. SEPT4:BLK, SEPT4:CD1C, GAS6:BLK and/or GAS6:CD1C). A score is assigned to each ratio, the score being indicative of the likelihood of the latent TB infection transitioning into active TB disease, based on the ratio for the respective gene pair. The subject can be identified as having a latent TB infection that is likely to transition into active TB disease or that is not likely to transition into active TB disease based on the score or on the average of the scores.

公开(公告)号：US10663463B2

公开(公告)日：2020-05-26

申请号：US15124743

申请日：2014-11-03

Applicant: Stellenbosch University

Inventor： Leon Milner Theodore Dicks ,  Willem Jacobus Perold ,  Deon Nevwling ,  Thomas Stanley Van Den Heever

IPC: G01N33/543 ,  G01N33/551 ,  A61B5/1473 ,  A61B5/00 ,  G01N15/10 ,  G01N33/553

Abstract: A system and method for the detection and quantification of biomolecules by measuring a piezoelectric signal is described. The system comprises a plurality of elongate zinc oxide nanowires mounted generally parallel to each another on a semi conductive silicon substrate. The free ends of the nanowires are provided with biomolecules that are capable of associating with complementary biomolecules within a biological or water sample. Following incubation of the system in a sample, the association of molecules of interest with the immobilised biomolecules on the system results in the displacement of the zinc oxide nanowires. The displacement of the nanowires produces a piezoelectric voltage signal that is useful in diagnosing a pathogenic infection or the contamination of a sample.

公开(公告)号：US20200034949A1

公开(公告)日：2020-01-30

申请号：US16335702

申请日：2017-09-21

Applicant: STELLENBOSCH UNIVERSITY

Inventor： Adriaan van Niekerk ,  Dugal Jeremy Harris

IPC: G06T3/40 ,  G06T7/73

Abstract: A system and method for generating radiometrically corrected surface images is provided. This includes providing one or more surface images of a first resolution of a surface area in the form of digital images which has a surface image sensor measurement of an intensity value of radiation in a given wavelength band reflected from the surface for each pixel of the images. A reference image of a second resolution of a corresponding surface area is provided to the surface of the surface image. The reference image has a surface reflectance for each pixel of the reference image for an equivalent wavelength band to the given wavelength band of the surface image. A functional relationship is modelled which relates the surface image sensor measurement for pixels of a surface image to the surface reflectance for pixels of the reference image to provide an estimated surface reflectance for each pixel of the surface images.