公开(公告)号：US09151762B2

公开(公告)日：2015-10-06

申请号：US13376984

申请日：2010-06-11

Applicant: J. Silvio Gutkind ,  Vyomesh Patel ,  Alfredo Molinolo ,  Timothy D. Veenstra

Inventor： J. Silvio Gutkind ,  Vyomesh Patel ,  Alfredo Molinolo ,  Timothy D. Veenstra

IPC: G01N33/574 ,  G01N33/53 ,  G01N33/50 ,  C07K16/28

CPC classification number: G01N33/57484 ,  C07K16/28 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/53 ,  G01N33/57407 ,  G01N2333/705

Abstract: Disclosed herein is a method of detecting metastasis of a tumor in a subject, such as metastasis to a lymph node. The method includes directly determining an amount of desmoglein-3 (DSG-3) protein in a sample from the subject (such as a lymph node sample) and comparing the amount of DSG-3 protein to a control, wherein an increase in the amount of DSG-3 protein in the sample relative to the control indicates metastasis of the tumor to the lymph node. The disclosed methods further include selecting a therapy (for example, surgery, lymph node dissection, radiation therapy, chemotherapy, or a combination of two or more thereof) for the subject based on the amount of DSG-3 protein in the sample from the subject.

Abstract translation: 本文公开了一种检测受试者中肿瘤转移的方法，例如转移到淋巴结。 该方法包括直接测定来自受试者（例如淋巴结样品）的样品中脱骨髓蛋白-3（DSG-3）蛋白的量，并将DSG-3蛋白的量与对照相比较，其中增加量 的DSG-3蛋白在样本中相对于对照表明肿瘤转移到淋巴结。 所公开的方法还包括基于来自受试者的样品中的DSG-3蛋白的量来选择受试者的治疗（例如，手术，淋巴结清扫，放射治疗，化学疗法或其两种或更多种的组合） 。

公开(公告)号：US20120009201A1

公开(公告)日：2012-01-12

申请号：US13257580

申请日：2009-03-20

Applicant: Josip Blonder ,  Bih-Rong Wei ,  Donald J. Johann ,  Timothy D. Veenstra

Inventor： Josip Blonder ,  Bih-Rong Wei ,  Donald J. Johann ,  Timothy D. Veenstra

IPC: A61K39/395 ,  C12Q1/68 ,  C12Q1/37 ,  G01N33/53 ,  G01N33/574 ,  C07K16/40 ,  C07K16/18 ,  C07H21/00 ,  A61P35/00 ,  C40B30/04 ,  A61K31/713

CPC classification number: G01N33/50 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/16 ,  G01N33/574 ,  G01N33/57438 ,  G01N33/57484 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2500/00

Abstract: Disclosed herein is a method of identifying a tumor biomarker. In one example, a tumor biomarker is identified by obtaining a peripheral biological fluid sample from a subject with a tumor as well as a tumor sample and an adjacent non-tumor sample from such subject. A protein expression profile is detected in the peripheral biological fluid sample, tumor sample and adjacent non-tumor sample. The protein expression profiles of the peripheral biological fluid sample, tumor sample and adjacent non-tumor sample are then compared, wherein an increase in expression of a specific protein in the tumor sample and peripheral biological fluid sample but not in the adjacent non-tumor sample indicates that the specific protein is a biomarker of the tumor. Also disclosed herein is a gene profiling signature that can be used to diagnosis a subject with renal cell carcinoma (RCC) or to identify agents with therapeutic potential to treat RCC. Thus, methods of diagnosing a subject with RCC are disclosed. Methods are also provided for identifying agents that alter an activity of a RCC biomarker.

Abstract translation: 本文公开了鉴定肿瘤生物标志物的方法。 在一个实例中，通过从具有肿瘤的受试者获得外周生物流体样品以及来自该受试者的相邻非肿瘤样品和肿瘤样品以及相邻的非肿瘤样品来鉴定肿瘤生物标志物。 在外周生物液体样品，肿瘤样品和相邻的非肿瘤样品中检测到蛋白质表达谱。 然后比较外周生物液体样品，肿瘤样品和相邻非肿瘤样品的蛋白质表达谱，其中肿瘤样品和外周生物液体样品中特异性蛋白质的表达增加，但不在相邻的非肿瘤样品中 表明特异性蛋白质是肿瘤的生物标志物。 本文还公开了可用于诊断患有肾细胞癌（RCC）的受试者或鉴定具有治疗潜力治疗RCC的药物的基因分型特征。 因此，公开了用RCC诊断受试者的方法。 还提供了用于鉴定改变RCC生物标志物活性的试剂的方法。

公开(公告)号：US20090148505A1

公开(公告)日：2009-06-11

申请号：US11955755

申请日：2007-12-13

Applicant: Susan K. Keay ,  Christopher Michejda ,  Zoltan Szekely ,  Thomas P. Conrads ,  Timothy D. Veenstra ,  Maria Michejda

Inventor： Susan K. Keay ,  Christopher Michejda ,  Zoltan Szekely ,  Thomas P. Conrads ,  Timothy D. Veenstra ,  Maria Michejda

IPC: A61K9/127 ,  A61K38/08 ,  A61K35/00 ,  A61K9/14 ,  A61K39/395

CPC classification number: C07K9/005 ,  A61K38/00

Abstract: A novel antiproliferative factor comprising a glycopeptide is disclosed. In specific embodiments, the novel antiproliferative factor is associated with the bladder. Compositions, diagnostic kits and reagents, and methods of using the compounds for identifying and/or treating interstitial cystitis and cancer are disclosed.

Abstract translation: 公开了包含糖肽的新型抗增殖因子。 在具体实施方案中，新型抗增殖因子与膀胱相关。 公开了组合物，诊断试剂盒和试剂以及使用该化合物鉴定和/或治疗间质性膀胱炎和癌症的方法。

公开(公告)号：US06818454B2

公开(公告)日：2004-11-16

申请号：US09788286

申请日：2001-02-16

Applicant: Michael B. Goshe ,  Thomas P. Conrads ,  Timothy D. Veenstra ,  Ellen A. Panisko

Inventor： Michael B. Goshe ,  Thomas P. Conrads ,  Timothy D. Veenstra ,  Ellen A. Panisko

IPC: C07K122

CPC classification number: G01N33/6842 ,  G01N33/6803 ,  Y10S530/811 ,  Y10T436/13 ,  Y10T436/24

Abstract: The invention provides reagents and methods for characterizing (i.e., identification and/or quantitation) the phosphorylation states of proteins. Proteins may be post-transcriptionally modified such that they contain phosphate groups at either some or all of their serine, threonine, tyrosine, histidine, and/or lysine amino acid residues. In many cases the extent to which a protein is phosphorylated determines it bioactivity, i.e., its ability to effect cell functions such as differentiation, division, and metabolism. Hence, a powerful tool for diagnosing various diseases and for furthering the understanding of protein—protein interactions is provided.

Abstract translation: 本发明提供用于表征（即，鉴定和/或定量）蛋白质的磷酸化状态的试剂和方法。 蛋白质可以进行后转录修饰，使得它们在丝氨酸，苏氨酸，酪氨酸，组氨酸和/或赖氨酸氨基酸残基的一部分或全部含有磷酸基。 在许多情况下，蛋白质被磷酸化的程度决定了其生物活性，即其影响细胞功能如分化，分裂和代谢的能力。 因此，提供了用于诊断各种疾病和促进对蛋白质 - 蛋白质相互作用的理解的强大工具。

公开(公告)号：US08790869B2

公开(公告)日：2014-07-29

申请号：US13257580

申请日：2009-03-20

Applicant: Josip Blonder ,  Bih-Rong Wei ,  Donald J. Johann ,  Timothy D. Veenstra

Inventor： Josip Blonder ,  Bih-Rong Wei ,  Donald J. Johann ,  Timothy D. Veenstra

IPC: G01N33/574 ,  G01N33/50 ,  G01N33/68

CPC classification number: G01N33/50 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/16 ,  G01N33/574 ,  G01N33/57438 ,  G01N33/57484 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2500/00

Abstract: Disclosed herein is a method of identifying a tumor biomarker. In one example, a tumor biomarker is identified by obtaining a peripheral biological fluid sample from a subject with a tumor as well as a tumor sample and an adjacent non-tumor sample from such subject. A protein expression profile is detected in the peripheral biological fluid sample, tumor sample and adjacent non-tumor sample. The protein expression profiles of the peripheral biological fluid sample, tumor sample and adjacent non-tumor sample are then compared, wherein an increase in expression of a specific protein in the tumor sample and peripheral biological fluid sample but not in the adjacent non-tumor sample indicates that the specific protein is a biomarker of the tumor. Also disclosed herein is a gene profiling signature that can be used to diagnosis a subject with renal cell carcinoma (RCC) or to identify agents with therapeutic potential to treat RCC. Thus, methods of diagnosing a subject with RCC are disclosed. Methods are also provided for identifying agents that alter an activity of a RCC biomarker.

Abstract translation: 本文公开了鉴定肿瘤生物标志物的方法。 在一个实例中，通过从具有肿瘤的受试者获得外周生物流体样品以及来自该受试者的相邻非肿瘤样品和肿瘤样品以及相邻的非肿瘤样品来鉴定肿瘤生物标志物。 在外周生物液体样品，肿瘤样品和相邻的非肿瘤样品中检测到蛋白质表达谱。 然后比较外周生物液体样品，肿瘤样品和相邻非肿瘤样品的蛋白质表达谱，其中肿瘤样品和外周生物液体样品中特异性蛋白质的表达增加，但不在相邻的非肿瘤样品中 表明特异性蛋白质是肿瘤的生物标志物。 本文还公开了可用于诊断患有肾细胞癌（RCC）的受试者或鉴定具有治疗潜力治疗RCC的药物的基因分型特征。 因此，公开了用RCC诊断受试者的方法。 还提供了用于鉴定改变RCC生物标志物活性的试剂的方法。

公开(公告)号：US20090215111A1

公开(公告)日：2009-08-27

申请号：US12272015

申请日：2008-11-17

Applicant: Timothy D. Veenstra ,  Xia Xu ,  Josip Blonder ,  Donald J. Johann, JR. ,  Regina G. Ziegler

Inventor： Timothy D. Veenstra ,  Xia Xu ,  Josip Blonder ,  Donald J. Johann, JR. ,  Regina G. Ziegler

IPC: G01N1/30

CPC classification number: G01N33/743

Abstract: Mass-spectrometry based methods of analyzing estrogens and other steroids from biological tissue sections samples are disclosed herein. Methods of detecting a disease state or condition or elevated risk of a disease state or condition in a mammal from tissue sections are also disclosed.

Abstract translation: 本文公开了基于质谱法分析来自生物组织切片样品​​的雌激素和其它类固醇的方法。 还公开了从组织切片检测哺乳动物的疾病状态或状况或疾病状态或病症风险升高的方法。

公开(公告)号：US07544932B2

公开(公告)日：2009-06-09

申请号：US10529967

申请日：2003-10-20

Applicant: George Janini ,  Haleem J Issaq ,  Timothy D Veenstra ,  Thomas P Conrads ,  Kenneth L Wilkens

Inventor： George Janini ,  Haleem J Issaq ,  Timothy D Veenstra ,  Thomas P Conrads ,  Kenneth L Wilkens

IPC: H01J49/00

CPC classification number: H01J49/04 ,  B05B5/0255 ,  B05B5/0533 ,  G01N27/44717 ,  G01N35/1095 ,  H01J49/167

Abstract: Contiguous capillaries useful for separating and electrospraying a fluid comprising analyte and electrolyte are provided. The contiguous capillaries have spray tips at one end of the capillaries and electrically conductive portions in proximity to the spray tips. Methods for making the contiguous capillaries and their use as electrospray sources are also disclosed. Apparatus and methods for conveying analyte ions from the capillaries into analytical instruments, such as a mass spectrometer, are also disclosed. The disclosed contiguous capillaries may be used to carryout electrophoresis separation and electrospray ionization of analytes. Methods for obtaining the mass spectra of macromolecular analytes at concentrations lower than previously possibly are provided using the apparatus and procedures described herein.

Abstract translation: 提供了可用于分离和电喷雾包含分析物和电解质的流体的连续毛细管。 连续的毛细管在毛细管的一端和靠近喷雾尖端的导电部分具有喷雾尖端。 还公开了制造连续毛细管的方法及其作为电喷雾源的用途。 还公开了将分析物离子从毛细管输送到诸如质谱仪之类的分析仪器中的装置和方法。 所公开的连续毛细管可用于进行分析物的电泳分离和电喷雾电离。 使用本文所述的装置和程序提供了以下可能获得浓度低于以前浓度的大分子分析物的质谱的方法。

公开(公告)号：US20120087892A1

公开(公告)日：2012-04-12

申请号：US13376984

申请日：2010-06-11

Applicant: J. Silvio Gutkind ,  Vyomesh Patel ,  Alfredo Molinolo ,  Timothy D. Veenstra

Inventor： J. Silvio Gutkind ,  Vyomesh Patel ,  Alfredo Molinolo ,  Timothy D. Veenstra

IPC: A61K38/20 ,  A61P35/04 ,  A61N5/00 ,  A61B17/32 ,  G01N33/574 ,  G01N27/447 ,  B82Y5/00 ,  B82Y99/00

CPC classification number: G01N33/57484 ,  C07K16/28 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/53 ,  G01N33/57407 ,  G01N2333/705

Abstract: Disclosed herein is a method of detecting metastasis of a tumor in a subject, such as metastasis to a lymph node. The method includes directly determining an amount of desmoglein-3 (DSG-3) protein in a sample from the subject (such as a lymph node sample) and comparing the amount of DSG-3 protein to a control, wherein an increase in the amount of DSG-3 protein in the sample relative to the control indicates metastasis of the tumor to the lymph node. The disclosed methods further include selecting a therapy (for example, surgery, lymph node dissection, radiation therapy, chemotherapy, or a combination of two or more thereof) for the subject based on the amount of DSG-3 protein in the sample from the subject.

Abstract translation: 本文公开了一种检测受试者中肿瘤转移的方法，例如转移到淋巴结。 该方法包括直接测定来自受试者（例如淋巴结样品）的样品中脱骨髓蛋白-3（DSG-3）蛋白的量，并将DSG-3蛋白的量与对照相比较，其中增加量 的DSG-3蛋白在样本中相对于对照表明肿瘤转移到淋巴结。 所公开的方法还包括基于来自受试者的样品中的DSG-3蛋白的量来选择受试者的治疗（例如，手术，淋巴结清扫，放射治疗，化学疗法或其两种或更多种的组合） 。