公开(公告)号：US20230400395A1

公开(公告)日：2023-12-14

申请号：US18328722

申请日：2023-06-03

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Bonnie Alden ,  Mame Maissa Gaye

IPC: G01N1/40 ,  C12Q1/6806

CPC classification number: G01N1/4055 ,  C12Q1/6806

Abstract: The present disclosure is directed to methods of sample preparation from lipid nanoparticle (LNP)-encapsulated nucleic acids utilizing solid-phase extraction techniques.

公开(公告)号：US20230295636A1

公开(公告)日：2023-09-21

申请号：US18151297

申请日：2023-01-06

Applicant: Waters Technologies Corporation

Inventor： Xiaoxiao Liu ,  Matthew A. Lauber

IPC: C12N15/115 ,  C07K16/28 ,  G01N33/561 ,  G01N33/68

CPC classification number: C12N15/115 ,  C07K16/283 ,  G01N33/561 ,  G01N33/6848 ,  G01N2560/00

Abstract: A method of capturing human immunoglobulin Fc domains in a biofluid sample is provided. The method includes providing an affinity capture device. The affinity capture device includes a surface having an aptamer that is at least 80% identical to SEQ ID NO 1 immobilized onto the surface of the affinity capture device. The biofluid sample is diluted with a binding buffer. The binding buffer includes (A) tris(hydroxymethyl)aminomethane (Tris), trimethylamine (TES), 2-ethanesulfonic acid (MES), or 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES); (B) a magnesium cation at a concentration between about 10 µM to about 20 mM; and (C) a total monovalent cation concentration from 0 to no greater than 100 mM. The human immunoglobulin Fc domains in the biofluid sample are adsorbed to the aptamer with the binding buffer.

公开(公告)号：US20230256413A1

公开(公告)日：2023-08-17

申请号：US18138991

申请日：2023-04-25

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Paul Rainville ,  Jacob N. Fairchild ,  Babjide Okandeji ,  Nicole L. Lawrence ,  Dimple Shah

IPC: B01J20/288 ,  B01D15/32 ,  B01J20/287 ,  B01D15/36 ,  B01D15/38 ,  B01J20/28 ,  B01J20/283

CPC classification number: B01J20/288 ,  B01D15/327 ,  B01J20/287 ,  B01D15/36 ,  B01D15/3847 ,  B01J20/28061 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/283 ,  B01J2220/54 ,  B01J2220/80

Abstract: The present invention provides the use of charged surface reversed phase chromatographic materials along with standard reversed-phase LC and mass spectrometry compatible conditions for the retention, separation, purification, and characterization of acidic, polar molecules, including, but not limited to, organic acids, α-amino acids, phosphate sugars, nucleotides, other acidic, polar biologically relevant molecules. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

公开(公告)号：US11709155B2

公开(公告)日：2023-07-25

申请号：US16133089

申请日：2018-09-17

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Mathew H. DeLano ,  Scott A. McCall ,  Jonathan L. Belanger ,  Theodore A. Dourdeville ,  Kerri M. Smith ,  Paul D. Rainville ,  Dimple D. Shah ,  Stephen J. Shiner ,  Catalin Doneanu ,  Michael Donegan

IPC: G01N30/56 ,  B01J20/32 ,  B05D1/00 ,  B01J20/291 ,  C23C16/04 ,  C23C16/30 ,  C23C14/04 ,  C23C14/12 ,  C23C14/24 ,  G01N30/60 ,  G01N30/72

CPC classification number: G01N30/56 ,  B01J20/291 ,  B01J20/3272 ,  B05D1/60 ,  C23C14/046 ,  C23C14/12 ,  C23C14/24 ,  C23C16/045 ,  C23C16/30 ,  G01N30/6052 ,  B05D2518/10 ,  G01N30/7266 ,  G01N2030/567

Abstract: A device for separating analytes is disclosed. The device has a sample injector, sample injection needle, sample reservoir container in communication with the sample injector, chromatography column downstream of the sample injector, and fluid conduits connecting the sample injector and the column. The interior surfaces of the fluid conduits, sample injector, sample reservoir container, and column form a flow path having wetted surfaces. A portion of the wetted surfaces of the flow path are coated with an alkylsilyl coating that is inert to at least one of the analytes. The alkylsilyl coating has the Formula I:




R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20—, —(C1-C10)[NH(CO)NH(C1-C10)]1-20—, or —(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

公开(公告)号：US20230201744A1

公开(公告)日：2023-06-29

申请号：US18179835

申请日：2023-03-07

Applicant: Waters Technologies Corporation

Inventor： Raymond P. Fisk ,  Timothy J. Becker ,  Mary Elizabeth Lame ,  Frank John Marszaikowski ,  Matthew A. Lauber

IPC: B01D15/38 ,  B01D15/42 ,  B01D15/14 ,  B01D15/12 ,  B01D15/22 ,  B01L3/00

CPC classification number: B01D15/3809 ,  B01D15/424 ,  B01D15/14 ,  B01D15/12 ,  B01D15/22 ,  B01L3/5023 ,  B01L2300/0681 ,  B01L2200/0631 ,  B01L3/5021 ,  B01L2400/0433 ,  B01L2400/0409 ,  B01L2300/069 ,  B01L2200/0668 ,  B01L3/0275

Abstract: The present disclosure pertains to sample preparation devices useful for affinity capture and purification that include one or more internal structures that comprise a reservoir, a well, a fluid passageway, sorbent particles, and a filter element that blocks passage of the affinity sorbent particles, which sample preparation devices combine the attributes of both dispersive and flow through designs into a single sample preparation device. The present disclosure also pertains to kits that contain and methods that use such sample preparation devices.

公开(公告)号：US20230149833A1

公开(公告)日：2023-05-18

申请号：US17988073

申请日：2022-11-16

Applicant: Waters Technologies Corporation

Inventor： Szabolcs Fekete ,  Michael O. Fogwill ,  Matthew A. Lauber

IPC: B01D15/42 ,  B01D15/16 ,  B01D15/22 ,  G01N30/32 ,  G01N30/72

CPC classification number: B01D15/422 ,  B01D15/163 ,  B01D15/22 ,  G01N30/32 ,  G01N30/72 ,  G01N2030/326 ,  G01N2030/8831

Abstract: There is provided a method of operating a liquid chromatography arrangement, the liquid chromatography arrangement comprising: a solvent pump arranged to flow a liquid solvent over a liquid chromatography column; a restrictor arranged to restrict the liquid solvent from leaving the liquid chromatography column; and a liquid pump arranged to provide liquid flow between the liquid chromatography column and the restrictor, the method comprising: flowing the liquid solvent through the liquid chromatography column using the solvent pump; and controlling a liquid pressure within the liquid chromatography column by providing a liquid flow between the liquid chromatography column and the restrictor using the liquid pump.

公开(公告)号：US20220268779A1

公开(公告)日：2022-08-25

申请号：US17741865

申请日：2022-05-11

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Darryl W. Brousmiche ,  Stephan M. Koza

IPC: G01N33/58 ,  G01N33/68 ,  C12P19/28

Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent. The derivizated glycosylamines can be separated from the reaction mixture and detected by chromatographic detection, fluorescence detection, mass spectrometry (“MS”), or Ultra Violet (“UV”) detection and/or a combination thereof.

公开(公告)号：US20220091079A1

公开(公告)日：2022-03-24

申请号：US17471463

申请日：2021-09-10

Applicant: Waters Technologies Corporation

Inventor： Amit Patel ,  Moon Chul Jung ,  Susan Rzewuski ,  Matthew A. Lauber ,  Thomas H. Walter ,  Kevin Wyndham ,  Mathew DeLano ,  Jennifer Simeone

IPC: G01N30/88

Abstract: The present disclosure is directed to a kit for evaluating system inertness. The kit includes a positive control comprising a metal interacting moiety, and a negative control that does not contain a metal interacting moiety. In some embodiments the kit also includes a container to hold a system suitability solution (e.g., an equimolar mixture of the positive control and the negative control).

公开(公告)号：US20220082531A1

公开(公告)日：2022-03-17

申请号：US17471393

申请日：2021-09-10

Applicant: Waters Technologies Corporation

Inventor： Amit Patel ,  Moon Chul Jung ,  Susan Rzewuski ,  Thomas H. Walter ,  Matthew A. Lauber ,  Kevin Wyndham ,  Mathew DeLano ,  Jennifer Simeone

IPC: G01N30/04

Abstract: The present disclosure is directed to methods of characterizing a system containing a chromatographic column. The methods can include introducing a sample comprising a positive control and a negative control to the system containing a chromatographic column, wherein the positive control is a sensitive probe that interacts with the system and the negative control is substantially non-interacting with the system; after passing the sample through the chromatographic column, detecting the positive control and the negative control; and determining system suitability by comparing the amount of detected positive control to negative control. In some embodiments, determining system suitability (e.g., inertness of sample to the system) is accomplished by determining a ratio of detected positive control to negative control.

公开(公告)号：US11235308B2

公开(公告)日：2022-02-01

申请号：US17080081

申请日：2020-10-26

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Daniel P. Walsh

IPC: B01J20/286 ,  B01J20/32 ,  C07F7/08 ,  B01J20/289 ,  B01J20/10 ,  B01J20/22 ,  B01J20/283

Abstract: Provided herein are stationary phase compositions comprising a chromatographic surface of porous or non-porous core material comprising a surface modifier for use in chromatographic separations.