公开(公告)号：US20250002528A1

公开(公告)日：2025-01-02

申请号：US18709049

申请日：2022-11-29

Applicant: Oxford University Innovation Limited

Inventor： Mark HOWARTH ,  Irsyad Khairil ANUAR ,  Rolle RAHIKAINEN

IPC: C07K1/22 ,  B01D15/20 ,  B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01J20/288 ,  C07K14/00 ,  C07K17/08

Abstract: The present invention relates to an affinity purification system comprising a polypeptide (protein) that binds selectively (e.g. specifically) and reversibly to its cognate peptide tag (ligand). In particular, the polypeptide comprises: (i) an amino acid sequence as set forth in SEQ ID NO: 1, wherein X at position 77 is selected from alanine, glycine, serine, asparagine, or threonine; (ii) a portion of (i) comprising an amino acid sequence as set forth in SEQ ID NO: 2, wherein X at position 56 is selected from alanine, glycine. serine, asparagine or threonine; (iii) an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 1, wherein the polypeptide comprises alanine, glycine, serine, asparagine or threonine at a position equivalent to position 77 of SEQ ID NO: 1. and wherein the poly peptide comprises histidine at positions equivalent to positions 30, 84 and 85 of SEQ ID NO: 1; or (iv) a portion of (iii) comprising an amino acid sequence with at least 80% sequence identity to a sequence as set forth in SEQ ID NO: 2, wherein the polypeptide comprises alanine, glycine, serine, asparagine or threonine at a position equivalent to position 56 of SEQ ID NO: 2, and wherein the polypeptide comprises histidine at positions equivalent to positions 9, 63 and 64 of SEQ ID NO: 2, wherein the polypeptide binds selectively and reversibly to a peptide comprising an amino acid sequence as set forth in SEQ ID NO: 3, 4 or 5.

公开(公告)号：US20240293799A1

公开(公告)日：2024-09-05

申请号：US18644518

申请日：2024-04-24

Applicant: Waters Technologies Corporation

Inventor： Kevin D. Wyndham ,  Michael F. Morris ,  Darryl W. Brousmiche ,  Jason F. Hill ,  Jacob N. Fairchild

IPC: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/32 ,  B01D15/40 ,  B01D53/02 ,  B01J20/22 ,  B01J20/28 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07F7/18

CPC classification number: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  C07F7/1804 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/32 ,  B01J20/3234 ,  B01J2220/54 ,  B01J2220/80

Abstract: In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

公开(公告)号：US20240198316A1

公开(公告)日：2024-06-20

申请号：US18556895

申请日：2022-05-30

Applicant: Suzhou Sepax Technologies, Inc.

Inventor： Huiming MAO ,  Feng XU ,  Ke YANG ,  Xinmei HU ,  Xueying HUANG

IPC: B01J20/288 ,  B01D15/20 ,  B01D15/32 ,  B01D15/34 ,  B01D15/36 ,  B01D15/38 ,  B01J20/26 ,  B01J20/28 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32

CPC classification number: B01J20/288 ,  B01D15/206 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28059 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/289 ,  B01J20/3064 ,  B01J20/3085 ,  B01J20/321 ,  B01J20/3221 ,  B01J20/3244 ,  B01J20/3293 ,  B01J2220/54

Abstract: A synthetic polymeric porous medium with a core-shell(s) hierarchical layer structure and has an essentially homogeneous porous structure from inside to outside of the medium, whose core and shell(s) are covalently modified with distinct chemical functional groups or same functional group with different density. Here the methodologies for resin syntheses and core-shell(s) modifications and liquid chromatographic applications of the newly developed resins in the field of analysis and purification of Tween surfactants, virus-like particles (VLP)/vaccines/viral vectors/viruses, antibody, and mRNA are disclosed.

公开(公告)号：US20210199624A1

公开(公告)日：2021-07-01

申请号：US17057361

申请日：2019-05-20

Applicant: Waters Technologies Corporation

Inventor： Martin Gilar ,  Thomas S. McDonald ,  Joseph D. Michienzi ,  Jeffrey Musacchio ,  Rachel Mumma ,  Keith Fadgen ,  Geoff C. Gerhardt

IPC: G01N30/06 ,  G01N1/40 ,  G01N30/54 ,  G01N30/60 ,  B01J20/24 ,  B01J20/28 ,  B01J20/288 ,  B01J20/32 ,  B01D15/38 ,  B01D15/22 ,  B01D15/16

Abstract: Sample preparation and separation can be performed using a sample cartridge (201). The cartridge includes a barrel (204) with a first and second end, a column segment (209) connected to the second end of the barrel, and a column (205) containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.

公开(公告)号：US20210163529A1

公开(公告)日：2021-06-03

申请号：US16955119

申请日：2018-12-13

Applicant: Cytiva Bioprocess R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Ronnie Palmgren ,  Tomas Bjorkman

IPC: C07K1/22 ,  B01J20/24 ,  B01J20/28 ,  B01J20/288 ,  B01J20/32 ,  B01J20/30 ,  C07K16/00 ,  B01D15/38

Abstract: A method for preparation of a separation matrix, comprising the steps of: a) providing a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein; b) reacting said alkali-stable ligand with said solid support to form a separation matrix having covalently coupled alkali-stable ligands; and c) washing said separation matrix having covalently coupled alkali-stable ligands with a wash solution comprising at least 10 mM of an alkali metal hydroxide.

公开(公告)号：US20210154642A1

公开(公告)日：2021-05-27

申请号：US16625992

申请日：2018-08-31

Applicant: TORAY INDUSTRIES, INC.

Inventor： Ryo MATSUNAGA ,  Junichi KASUYA ,  Yoshiyuki UENO ,  Hirofumi YAMANAKA ,  Yasunori KANEMORI ,  Masato MASUDA ,  Joji FUNAKOSHI

IPC: B01J20/26 ,  D01F8/04 ,  B01D15/38 ,  A61M1/36 ,  B01J20/28 ,  B01J20/288

Abstract: An object of the present invention is to provide a ligand-immobilized sea-island composite fiber in which generation of fine particles due to peeling of a sea component from an island component and generation of fine particles due to destruction of a fragile sea component are both suppressed. The present invention provides a sea-island composite fiber comprising a sea component and island components, in which a value (L/S) obtained by dividing the average total length (L) of the perimeter of all island components in a cross section perpendicular to the fiber axis by the average cross-sectional area (S) of the cross section is from 1.0 to 50.0 μm−1, a distance from the surface to the outermost island component is 1.9 μm or less, and an amino group-containing compound is covalently bonded to a polymer constituting the sea component at a charge density of 0.1 μmol or more and less than 500 μmol per 1 gram dry weight.

公开(公告)号：US20210101128A1

公开(公告)日：2021-04-08

申请号：US17044393

申请日：2018-12-28

Applicant: SHIMADZU CORPORATION

Inventor： Tadayuki YAMAGUCHI ,  Daiki FUJIMURA ,  Hideyuki OTSUKI

IPC: B01J20/24 ,  B01J20/10 ,  B01J20/28 ,  B01J20/288 ,  G01N30/72 ,  B01J20/281 ,  G01N30/16

Abstract: A separating column (12) for hippuric acid analysis is filled with a filler in which 123 μmol/g or more of β-cyclodextrin is chemically bonded to a silica matrix. By using such a filler for the separating column (12) in which 123 μmol/g or more of β-cyclodextrin is chemically bonded to the silica matrix, hippuric acid, o-methyl hippuric acid, m-methyl hippuric acid, p-methyl hippuric acid, and mandelic acid can be separated without using a mobile phase containing cyclodextrin.

公开(公告)号：US20200262873A1

公开(公告)日：2020-08-20

申请号：US16649889

申请日：2018-09-25

Applicant: JSR CORPORATION ,  JSR Life Sciences Corporation ,  JSR Micro Inc. ,  JSR Micro N.V.

Inventor： Jun-ichi YASUOKA ,  Kiichi YOSHIMURA ,  Tomoaki HAGA

IPC: C07K14/195 ,  C12N5/10 ,  C12N15/63 ,  G01N33/543 ,  C07K1/22 ,  B01J20/24 ,  B01J20/288 ,  B01J20/30 ,  B01D15/38

Abstract: Provided are a protein L-derived immunoglobulin binding protein having an increased antibody dissociation rate under acidic conditions, and an affinity support using the same. Disclosed are an immunoglobulin binding protein comprising at least one mutant of an immunoglobulin binding domain, and an affinity support comprising a solid-phase support having the immunoglobulin binding protein bound thereto. A mutant of the immunoglobulin binding domain consists of an amino acid sequence having an identity of at least 85% with the sequence set forth in any one of SEQ ID NO:1 to SEQ ID NO:9 and a predetermined mutation, and the mutant has immunoglobulin κ chain binding activity.

公开(公告)号：US10744484B2

公开(公告)日：2020-08-18

申请号：US16058069

申请日：2018-08-08

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Jacob N. Fairchild ,  Jason F. Hill ,  Giorgis Isaac ,  Michael F. Morris ,  Kevin D. Wyndham

IPC: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/40 ,  B01J20/22 ,  B01J20/32 ,  C07F7/18 ,  B01J20/288 ,  B01J20/28 ,  B01D15/32 ,  B01D53/02 ,  B01J20/289 ,  B01J20/30 ,  B01J20/285 ,  B01J20/284 ,  B01J20/282

Abstract: The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. In some examples, the present disclosure can include a chromatographic system having a chromatographic column having a stationary phase with a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or a combination thereof.

公开(公告)号：US10695747B2

公开(公告)日：2020-06-30

申请号：US15560962

申请日：2016-04-01

Applicant: DAICEL CORPORATION

Inventor： Toru Shibata ,  Yasuto Morishita ,  Keiji Nishihara

IPC: B01J20/285 ,  B01J20/286 ,  C07K1/22 ,  C08J9/28 ,  B01D15/38 ,  C08B15/00 ,  B01J20/30 ,  B01J20/24 ,  B01J20/26 ,  B01J20/288

Abstract: Provided are a technique for preparing a porous cellulose medium without using a special gelling agent for a solution in which cellulose acetate serving as a raw material is dissolved; and a porous cellulose medium and the like produced using the technique. A method for producing a porous cellulose medium comprises the step of preparing a flowable homogeneous composition comprising cellulose acetate, a basic compound, and a solvent including water, and gelling the composition by deacetylation reaction of the cellulose acetate.