公开(公告)号：US20190225646A1

公开(公告)日：2019-07-25

申请号：US16339119

申请日：2017-10-03

Applicant: Spiber Inc.

Inventor： Toshimasa Homma

IPC: C07K1/30 ,  C07K14/78 ,  D01F6/68 ,  D01D1/02 ,  D01D5/06

Abstract: Disclosed is a method for purifying a recombinant protein of interest from a recombinant cell expressing intracellularly the recombinant protein as an insoluble matter, comprising treating the recombinant cell under conditions where a protein derived from a host cell dissolves in a first aprotic polar solvent to which an inorganic salt is added or not but the recombinant protein does not dissolve, removing a first soluble fraction, and then treating the resulting first insoluble fraction under conditions where the recombinant protein dissolves in a second aprotic polar solvent to which an inorganic salt is added.

公开(公告)号：US20190031842A1

公开(公告)日：2019-01-31

申请号：US16073069

申请日：2017-01-27

Applicant: Riken ,  Spiber Inc. ,  Kojima Industries Corporation

Inventor： Keiji Numata ,  Kana Ishida ,  Hironori Yamamoto

IPC: C08J5/18 ,  C07K14/435

Abstract: The present invention provides, in one aspect, a method for producing a molded article, the method comprising exposing a molded article precursor comprising a protein to an environment with a relative humidity of 80% or more to obtain the molded article.

公开(公告)号：US20180251501A1

公开(公告)日：2018-09-06

申请号：US15760492

申请日：2016-09-08

Applicant: Spiber Inc. ,  Kojima Industries Corporation ,  Tekunohama Co., Ltd.

Inventor： Junichi Sugahara ,  Kaori Sekiyama ,  Ayumi Abe ,  Junichi Shimokata ,  Junichi Noba ,  Shinji Hirai

IPC: C07K14/435 ,  B29C43/52

CPC classification number: C07K14/43518 ,  B29C43/003 ,  B29C43/34 ,  B29C43/52 ,  B29K2089/00 ,  C07K14/435 ,  C08H1/00 ,  C08J5/00 ,  C12N15/09

Abstract: Provided is a molded article of a composition comprising a polypeptide which has more than 4.3 GPa of a bending elastic modulus and transparency.

公开(公告)号：US09689089B2

公开(公告)日：2017-06-27

申请号：US14405101

申请日：2013-04-26

Applicant: SPIBER INC.

Inventor： Mizuki Ishikawa ,  Kazuhide Sekiyama

IPC: D01F4/02 ,  D01F4/00 ,  D01F1/06 ,  C07K14/43 ,  D01D5/04 ,  D01D5/06 ,  C07K14/435 ,  D01D1/02

CPC classification number: D01F4/02 ,  C07K14/43518 ,  D01D1/02 ,  D01D5/04 ,  D01D5/06 ,  D01F1/06 ,  D01F4/00

Abstract: A solution-dyed protein fiber of the present invention includes 0-100 mass % of silk fibroin and 100-0 mass % of a polypeptide derived from spider silk proteins when the protein fiber is assumed to be 100 mass %, wherein the solution-dyed protein fiber contains a solution-dyeing colorant. The fiber is obtained by: dissolving or dispersing a solution-dyeing colorant in a solvent used for a spinning solution or in dimethyl sulfoxide, thereby preparing a coloring liquid; adding a solvent to the coloring liquid in an amount necessary for a spinning solution; adding and dissolving protein powder into the solvent, thereby preparing a spinning solution; and subjecting the spinning solution to wet spinning or dry-wet spinning. Thereby, the present invention provides a low-cost solution-dyed protein fiber in which a solution-dyeing colorant is dispersed uniformly and that can exhibit bright color tone, and a method for producing the same.

公开(公告)号：US20170066805A1

公开(公告)日：2017-03-09

申请号：US15356915

申请日：2016-11-21

Applicant: AJINOMOTO CO., INC. ,  SPIBER INC.

Inventor： Kentaro Nakase

IPC: C07K14/435 ,  C12P21/02 ,  C12N15/70

Abstract: A method for producing a fibroin-like protein is described. A fibroin-like protein is produced by culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein the cell proliferation after inducing the expression is reduced.

Abstract translation: 描述了一种生产丝心蛋白样蛋白的方法。 通过培养在培养基中培养具有编码丝心蛋白样蛋白质的基因的大肠杆菌，诱导编码丝心蛋白样蛋白质的基因的表达，收集丝心蛋白样蛋白质，产生丝心蛋白样蛋白质，诱导后的细胞增殖 表达式减少。

公开(公告)号：US20150374833A1

公开(公告)日：2015-12-31

申请号：US14764455

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki OSAWA ,  Keisuke MORITA

IPC: A61K47/42 ,  A61L27/22 ,  A61L27/52 ,  A61K38/17

CPC classification number: A61K47/42 ,  A61K9/0087 ,  A61K38/1767 ,  A61L26/008 ,  A61L27/227 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/52 ,  A61L27/60 ,  A61L31/145 ,  A61L2430/06 ,  C07K14/43518

Abstract: A polypeptide hydrogel of the present invention is a hydrogel of a polypeptide derived from spider silk proteins, and has a moisture content of 85.0 to 99.9 mass % based on 100 mass % of the polypeptide hydrogel. A method for producing the hydrogel includes: a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide; and a substitution step in which the solution produced in the solution production step is substituted with a water-soluble solvent. Thus, the present invention provides a polypeptide hydrogel having excellent water retentivity and a polypeptide hydrogel suitable for application to a living body.

Abstract translation: 本发明的多肽水凝胶是来自蜘蛛丝蛋白质的多肽的水凝胶，相对于多肽水凝胶100质量％，水分含量为85.0〜99.9质量％。 一种水凝胶的制造方法，其特征在于，将来自蜘蛛丝蛋白质的多肽溶解于至少一种溶解溶剂中的溶液制造工序，所述溶解溶剂选自：（A）二甲基亚砜; （B）二甲基亚砜与无机盐; 和（C）N，N-二甲基甲酰胺与无机盐反应，得到多肽的溶液; 以及其中在溶液制备步骤中产生的溶液被水溶性溶剂代替的取代步骤。 因此，本发明提供了具有优异的保水性的多肽水凝胶和适用于生物体的多肽水凝胶。

公开(公告)号：US20150344542A1

公开(公告)日：2015-12-03

申请号：US14655027

申请日：2013-12-18

Applicant: SPIBER INC.

Inventor： Toshiaki OSAWA ,  Keisuke MORITA

IPC: C07K14/78 ,  C07K14/435 ,  C07K1/14

CPC classification number: C07K14/78 ,  C07K1/14 ,  C07K1/145 ,  C07K14/43518 ,  C07K14/43563

Abstract: The present invention relates to a method for partially purifying a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a solution acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to dissolve the host cell and an insoluble substance is separated therefrom so as to obtain a solution. The hydrophilic recombinant protein has a hydropathy index of 0 or less, and the dissolving solvent is an aprotic polar solvent. Preferably, the solution is subjected to solvent substitution using an aqueous solvent.

Abstract translation: 本发明涉及从表达亲水重组蛋白的宿主部分纯化亲水重组蛋白的方法。 该方法包括：溶液获取步骤，其中向表达亲水重组蛋白的宿主中加入溶解溶剂以溶解宿主细胞，并从其中分离出不溶性物质以获得溶液。 亲水重组蛋白的亲水指数为0以下，溶解性溶剂为非质子极性溶剂。 优选地，使用水性溶剂对溶液进行溶剂置换。

公开(公告)号：US20140058066A1

公开(公告)日：2014-02-27

申请号：US14114636

申请日：2012-05-30

Applicant: SPIBER INC.

Inventor： Kazuhide Sekiyama ,  Kaori Sekiyama ,  Mizuki Ishikawa ,  Ryota Sato ,  Shinya Murata

IPC: C07K14/435

CPC classification number: C07K14/43518 ,  D01D5/06 ,  D01D5/16 ,  D01F4/02 ,  D01F6/68

Abstract: An artificial polypeptide fiber of the present invention is an artificial fiber containing a polypeptide as a main component, and has a stress of 350 MPa or more and a toughness of 138 MJ/m3 or more. A method for producing an artificial polypeptide fiber of the present invention is a method for producing the artificial polypeptide fiber obtained by spinning a spinning solution (6) containing a polypeptide derived from natural spider silk proteins and performing drawing of at least two stages. The drawing of at least two stages includes a first-stage drawing (3) in wet heat and a second-stage drawing (4) in dry heat. Thereby, the present invention provides high-toughness artificial polypeptide fibers having favorable stress and rupture elongation, and a method for producing the same.

Abstract translation: 本发明的人造多肽纤维是以多肽为主成分的人造纤维，其应力为350MPa以上，韧性为138MJ / m 3以上。 本发明的人造多纤维的制造方法是通过纺丝含有天然蜘蛛丝蛋白质的多肽的纺丝溶液（6）而得到的人造多纤维的制造方法，并进行至少两个阶段的拉伸。 至少两个阶段的绘图包括在湿热中的第一阶段绘图（3）和在干热中的第二阶段绘图（4）。 因此，本发明提供了具有良好的应力和断裂伸长率的高韧性人造多肽纤维及其制造方法。