公开(公告)号：US20090139908A1

公开(公告)日：2009-06-04

申请号：US11999171

申请日：2007-12-04

Applicant: Yuchen Zhou ,  Yimin Guo

Inventor： Yuchen Zhou ,  Yimin Guo

IPC: B03C1/02

CPC classification number: B03C1/033 ,  B03C1/034 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26

Abstract: Presented herein is a method and devices for identifying biological molecules and cells labeled by small magnetic particles and by optically active dyes. The labeled molecules are typically presented in a biological fluid but are then magnetically guided into narrow channels by a sequential process of magnetically trapping and releasing the magnetic labels that is implemented by sequential synchronized reversing the magnetic fields of a regular array of patterned magnetic devices that exert forces on the magnetic particles. These devices, which may be bonded to a substrate, can be formed as parallel magnetic strips adjacent to current carrying lines or can be substantially of identical structure to trilayered MTJ cells. Once the magnetically labeled molecules have been guided into the appropriate channels, their optical labels can be detected by a process of optical excitation and de-excitation. The molecules are thereby identified and counted.

Abstract translation: 本文提出的是用于鉴定由小磁性颗粒和光学活性染料标记的生物分子和细胞的方法和装置。 标记的分子通常呈现在生物流体中，然后通过磁捕获和释放磁性标签的顺序过程被磁性引导到窄通道中，磁性标记通过顺序同步反转正在施加的图案化磁性装置的规则阵列的磁场来实现 对磁性颗粒的力。 这些可以结合到衬底的器件可以形成为与载流线相邻的平行磁条，或者可以与三层MTJ电池基本上具有相同的结构。 一旦磁标记的分子被引导到适当的通道中，它们的光学标记可以通过光学激发和去激发的过程来检测。 因此鉴定和计数分子。

公开(公告)号：US20090095419A1

公开(公告)日：2009-04-16

申请号：US12335814

申请日：2008-12-16

Applicant: Kelly G. Ammann ,  Ralph E. Burns ,  Ernest V. Hansberry ,  Glenn A. Horner ,  Cheryl A. Jakub ,  John E. Kling ,  Donald J. Nieglos ,  Robert E. Schneider ,  Robert J. Smith

Inventor： Kelly G. Ammann ,  Ralph E. Burns ,  Ernest V. Hansberry ,  Glenn A. Horner ,  Cheryl A. Jakub ,  John E. Kling ,  Donald J. Nieglos ,  Robert E. Schneider ,  Robert J. Smith

IPC: B29C63/00

CPC classification number: C12Q1/6834 ,  B01F9/0001 ,  B01F9/0014 ,  B01F9/0021 ,  B01F2009/0074 ,  B01L7/52 ,  B01L7/5255 ,  B01L9/06 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2300/1827 ,  B03C1/282 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/26 ,  C12Q1/6813 ,  C12Q1/6832 ,  C12Q2537/10 ,  G01N35/0098 ,  G01N35/0099 ,  G01N35/025 ,  G01N35/1065 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/0437 ,  G01N2035/0455 ,  G01N2035/103 ,  Y10T29/53657 ,  Y10T29/53683 ,  Y10T29/53843 ,  Y10T156/1105 ,  Y10T156/1906 ,  Y10T436/11 ,  Y10T436/111666 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, performing an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step.

公开(公告)号：US20090067280A1

公开(公告)日：2009-03-12

申请号：US11875306

申请日：2007-10-19

Applicant: Kelly G. Ammann ,  Robert E. Schneider ,  Robert J. Smith

Inventor： Kelly G. Ammann ,  Robert E. Schneider ,  Robert J. Smith

IPC: B01F15/06

CPC classification number: C12Q1/6834 ,  B01F9/0001 ,  B01F9/0014 ,  B01F9/0021 ,  B01F2009/0074 ,  B01L7/52 ,  B01L7/5255 ,  B01L9/06 ,  B01L2200/147 ,  B01L2300/1822 ,  B01L2300/1827 ,  B03C1/282 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/26 ,  C12Q1/6813 ,  C12Q1/6832 ,  C12Q2537/10 ,  G01N35/0098 ,  G01N35/0099 ,  G01N35/025 ,  G01N35/1065 ,  G01N2035/00356 ,  G01N2035/00524 ,  G01N2035/0437 ,  G01N2035/0455 ,  G01N2035/103 ,  Y10T29/53657 ,  Y10T29/53683 ,  Y10T29/53843 ,  Y10T156/1105 ,  Y10T156/1906 ,  Y10T436/11 ,  Y10T436/111666 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163 ,  Y10T436/2575

Abstract: An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations, or modules, in which discrete aspects of the assay are performed on fluid samples contained in reaction receptacles. The analyzer includes stations for automatically preparing a specimen sample, incubating the sample at prescribed temperatures for prescribed periods, performing an analyte isolation procedure, and ascertaining the presence of a target analyte. An automated receptacle transporting system moves the reaction receptacles from one station to the next. The analyzer further includes devices for carrying a plurality of specimen tubes and disposable pipette tips in a machine-accessible manner, a device for agitating containers of target capture reagents comprising suspensions of solid support material and for presenting the containers for machine access thereto, and a device for holding containers of reagents in a temperature controlled environment and presenting the containers for machine access thereto. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte. The process is performed by automatically moving each of a plurality of reaction receptacles containing a solid support material and a fluid sample between stations for incubating the contents of the reaction receptacle and for separating the target analyte bound to the solid support from the fluid sample. An amplification reagent is added to the separated analyte after the analyte separation step and before a final incubation step.

Abstract translation: 用于执行多个诊断测定的自动分析仪同时包括多个站或模块，其中对反应容器中包含的流体样品进行分析方面的分析。 分析仪包括用于自动准备标本样品，在规定温度下规定时间孵育样品，进行分析物分离程序，以及确定目标分析物的存在的站。 自动插座输送系统将反应容器从一个站移动到另一个站。 分析仪还包括用于以机器可接近的方式携带多个样本管和一次性移液管尖端的装置，用于搅拌包含固体支持材料的悬浮液并用于提供用于机器通入的容器的靶捕获试剂的容器的装置，以及 用于在温度受控的环境中容纳试剂容器的装置，并且提供用于机器通入的容器。 执行自动诊断测定的方法包括用于分离和扩增靶分析物的自动化方法。 该过程通过在站之间自动移动包含固体支持材料和流体样品的多个反应容器中的每一个来进行，用于孵育反应容器的内容物并将与固体支持物结合的目标分析物与流体样品分离。 在分析物分离步骤之后和最终孵育步骤之前，将分离的分析物加入扩增试剂。

公开(公告)号：US20090050569A1

公开(公告)日：2009-02-26

申请号：US11906171

申请日：2007-09-28

Applicant: Edward K.Y. Jung ,  Eric C. Leuthardt ,  Royce A. Levien ,  Robert W. Lord ,  Mark A. Malamud ,  John D. Rinaldo, JR. ,  Lowell L. Wood, JR.

Inventor： Edward K.Y. Jung ,  Eric C. Leuthardt ,  Royce A. Levien ,  Robert W. Lord ,  Mark A. Malamud ,  John D. Rinaldo, JR. ,  Lowell L. Wood, JR.

IPC: B01D17/00 ,  B03C1/30

CPC classification number: B03C1/30 ,  B01L3/502776 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/043 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N1/4055 ,  G01N2001/4072

Abstract: The present disclosure relates to methods and devices that may be used to separate components from one or more samples.

Abstract translation: 本公开涉及可用于从一个或多个样品分离组件的方法和装置。

公开(公告)号：US20080302732A1

公开(公告)日：2008-12-11

申请号：US12126670

申请日：2008-05-23

Applicant: Hyongsok Soh ,  Brian Scott Ferguson ,  Yanting Zhang ,  Nancy Stagliano

Inventor： Hyongsok Soh ,  Brian Scott Ferguson ,  Yanting Zhang ,  Nancy Stagliano

IPC: B03C1/30 ,  G01N33/48

CPC classification number: B03C1/015 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0668 ,  B03C1/286 ,  B03C1/288 ,  B03C5/028 ,  B03C2201/18 ,  B03C2201/26

Abstract: A fluidic device employs one or more sorting stations for separating target species from other species in a sample. At least one of the sorting stations employs a magnetic field gradient to accomplish separation. In addition, the sorting station is integrated on a single substrate with one or more other modules for processing the sample. For example, the fluidic device may include both a sorting station and a separate trapping station that holds some or all components of the sample for additional processing. The trapping station may be located at a position upstream or downstream from the sorting module.

Abstract translation: 流体装置采用一个或多个分选站，用于将样品中的目标物种与其他物种分离。 至少一个分选站采用磁场梯度来实现分离。 此外，分选站集成在具有用于处理样品的一个或多个其他模块的单个基板上。 例如，流体装置可以包括分选站和分离收集站，分离站收集样品的一些或所有组分以用于附加处理。 捕集站可以位于分拣模块上游或下游的位置。

公开(公告)号：US20080190458A1

公开(公告)日：2008-08-14

申请号：US11704138

申请日：2007-02-08

Applicant: Luis Miguel Garcia Gros ,  Mark Talmer ,  Gerhardt P. Schroeder ,  Paul C. Dahlstrom ,  Jose Serra Domenech

Inventor： Luis Miguel Garcia Gros ,  Mark Talmer ,  Gerhardt P. Schroeder ,  Paul C. Dahlstrom ,  Jose Serra Domenech

IPC: B08B7/00 ,  B01L3/02 ,  B08B13/00

CPC classification number: G01N33/54333 ,  B03C1/0332 ,  B03C1/288 ,  B03C2201/20 ,  B03C2201/26 ,  G01N1/34

Abstract: Embodiments of the invention relate to a clinical instrument analyzer system for the automatic analysis of patient samples. In one embodiment, the analyzer may be used to analyze bodily fluid samples, such as blood, plasma, serum, urine or cerebrospinal fluid, for example. Embodiments of the invention relate to an apparatus and method, for example, an immunoassay method, for separating out target molecules in a magnetic field and then analyzing those target molecules with a luminometer.

Abstract translation: 本发明的实施例涉及一种用于患者样本的自动分析的临床仪器分析仪系统。 在一个实施例中，分析器可用于分析体液样品，例如血液，血浆，血清，尿液或脑脊髓液。 本发明的实施例涉及一种用于在磁场中分离目标分子然后用发光计分析这些目标分子的装置和方法，例如免疫测定方法。

公开(公告)号：US07390387B2

公开(公告)日：2008-06-24

申请号：US10808803

申请日：2004-03-25

Applicant: Winthrop D. Childers ,  David Tyvoll ,  Kirk Norton

Inventor： Winthrop D. Childers ,  David Tyvoll ,  Kirk Norton

IPC: G01N27/447 ,  G01N27/453

CPC classification number: B03C5/005 ,  B03C5/028 ,  B03C2201/26 ,  G01N2015/149

Abstract: A method of sorting cells comprises providing a fluid flow path for cell movement through a series of cell sorters in a first direction with an output of a preceding cell sorter of the series communicating with an input of a successive cell sorter of the series. Within each cell sorter, a first portion of the cells is separated from a second portion of the cells by applying a first non-uniform electric field via a first electrode array to cause movement of the first portion of the cells in a second direction across the fluid flow path, having a component generally transverse to the first direction into the output of a respective cell sorter.

Abstract translation: 一种分选细胞的方法包括提供流体流动路径，用于使细胞在第一方向上通过一系列细胞分选器移动，其中该序列的先前细胞分选器的输出与该系列的连续细胞分选器的输入通信。 在每个细胞分选器内，通过经由第一电极阵列施加第一非均匀电场，使细胞的第一部分与细胞的第二部分分离，以引起细胞的第一部分在第二方向上移动穿过 流体流动路径，具有通常横向于第一方向的分量进入相应的细胞分选器的输出。

公开(公告)号：US20070251885A1

公开(公告)日：2007-11-01

申请号：US10576297

申请日：2004-10-20

Applicant: Matti Korpela ,  Kenneth Rundt

Inventor： Matti Korpela ,  Kenneth Rundt

IPC: B01D35/06 ,  B01D15/02

CPC classification number: G01N1/28 ,  B03C1/286 ,  B03C2201/18 ,  B03C2201/26 ,  G01N33/54326 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: Method for handling microparticles in such a manner, that at least two treatment steps are performed for microparticles in the same vessel without moving the particles to another vessel. There are organs in the device for changing the solution without having to move the microparticles to another vessel.

Abstract translation: 以这样的方式处理微粒的方法，即对同一容器中的微粒进行至少两个处理步骤，而不将颗粒移动到另一个容器。 在用于改变溶液的装置中存在器官，而不必将微粒移动到另一个容器。

公开(公告)号：US20070175830A1

公开(公告)日：2007-08-02

申请号：US10564118

申请日：2004-07-05

Applicant: Lothar Brassard

Inventor： Lothar Brassard

IPC: C02F1/48

CPC classification number: B03C1/286 ,  B03C1/01 ,  B03C1/06 ,  B03C1/288 ,  B03C2201/26

Abstract: A device is provided to separate magnetic or magnetizable particles from a liquid by using a magnetic field. The device includes two limbs made of a soft-magnetic material. An air gap is provided between be two poles of the limbs. The air gap can receive one or more liquid containers. A head piece is arranged in a fixed or detachable manner on one of the two poles. One or more magnetizable bars are disposed in a fixed or movable manner on the head piece, in the vertical direction. One or more permanent magnets are arranged in a movable manner on at least one point of the device, such that a magnetic field can be produced between the two poles and the magnetic field can be activated or deactivated by moving the magnet(s). The region of the device where the movable magnet(s) are arranged is at least partially surrounded by a material that screens the magnetic field.

Abstract translation: 提供了一种通过使用磁场将磁性或可磁化颗粒与液体分离的装置。 该装置包括由软磁材料制成的两个肢体。 在四肢的两极之间设置气隙。 气隙可以容纳一个或多个液体容器。 头部件以固定或可拆卸的方式布置在两个极中的一个上。 一个或多个可磁化棒在垂直方向上以固定或可移动的方式设置在头部件上。 一个或多个永磁体以可移动方式布置在装置的至少一个点上，使得可以在两极之间产生磁场，并且可以通过移动磁体来激活或停用磁场。 可移动磁体布置的装置的区域至少部分被屏蔽磁场的材料所包围。

公开(公告)号：US20060201868A1

公开(公告)日：2006-09-14

申请号：US11076971

申请日：2005-03-11

Applicant: Blake Simmons ,  Gregory McGraw ,  Allen Salmi ,  Gregory Fiechtner ,  Eric Cummings ,  Yolanda Fintschenko

Inventor： Blake Simmons ,  Gregory McGraw ,  Allen Salmi ,  Gregory Fiechtner ,  Eric Cummings ,  Yolanda Fintschenko

IPC: B01D63/00

CPC classification number: B01D57/02 ,  B03C5/024 ,  B03C2201/26 ,  Y10T436/255

Abstract: Disclosed herein are methods and devices for assaying and concentrating analytes in a fluid sample using dielectrophoresis. As disclosed, the methods and devices utilize substrates having a plurality of pores through which analytes can be selectively prevented from passing, or inhibited, on application of an appropriate electric field waveform. The pores of the substrate produce nonuniform electric field having local extrema located near the pores. These nonuniform fields drive dielectrophoresis, which produces the inhibition. Arrangements of electrodes and porous substrates support continuous, bulk, multi-dimensional, and staged selective concentration.

Abstract translation: 本文公开了用于使用介电电泳测定和浓缩流体样品中的分析物的方法和装置。 如所公开的，所述方法和装置利用具有多个孔的衬底，通过适当的电场波形可以选择性地防止分析物通过或抑制分析物。 底物的孔隙产生具有位于孔附近的局部极值的不均匀电场。 这些不均匀的场驱动介电电泳，产生抑制作用。 电极和多孔基底的布置支持连续，体积，多维和分阶段的选择性浓度。