公开(公告)号：US20210109101A1

公开(公告)日：2021-04-15

申请号：US16884737

申请日：2020-05-27

Applicant: THE SCRIPPS RESEARCH INSTITUTE ,  COLD SPRING HARBOR LABORATORY

Inventor： Peter Kuhn ,  Angel Ernesto Dago Rodriguez ,  Anders Carlsson ,  Wei Liu ,  Jim Hicks

IPC: G01N33/574

Abstract: The present invention provides methods for predicting response to a hormone-directed therapy or chemotherapy in a prostate cancer (PCa) patient comprising (a) performing a direct analysis comprising immunofluorescent staining and morphological characteristization of nucleated cells in a blood sample obtained from the patient to identify and enumerate circulating tumor cells (CTC); (b) individually characterizing genotypic, morphometric and protein expression parameters to generate a profile for each of the CTCs, and (c) predicting response to hormone-directed therapy in the prostate cancer PCa patient based on said profile. In some embodiments, the methods comprise repeating steps (a) through (c) at one or more timepoints after initial diagnosis of prostate cancer to sequentially monitor said genotypic, morphometric and protein expression parameters.

公开(公告)号：US20200157557A1

公开(公告)日：2020-05-21

申请号：US16783613

申请日：2020-02-06

Applicant: E. I. DU PONT DE NEMOURS AND COMPANY ,  COLD SPRING HARBOR LABORATORY

Inventor： STEPHEN M ALLEN ,  DAVID PETER JACKSON ,  BYOUNG IL JE ,  MAI KOMATSU ,  YOUNG KOUNG LEE ,  HAJIME SAKAI

IPC: C12N15/82 ,  A01H5/10

Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.

公开(公告)号：US10253326B2

公开(公告)日：2019-04-09

申请号：US15086992

申请日：2016-03-31

Applicant: The United States of America, as represented by the Secretary of Agriculture ,  Cold Spring Harbor Laboratory

Inventor： Zhanguo Xin ,  Gloria B. Burow ,  Chad M Hayes ,  John J. Burke ,  Doreen Ware ,  Yinping Jiao

IPC: C12N15/82 ,  C12N9/02 ,  C12Q1/6895

Abstract: Genetically altered sorghum plants expressing the multi-seeded #2 phenotype contain one of two genomic alterations in the Sb06g018040 gene which result in reduced activity of the encoded protein, a class II 13-lipoxygenase. This phenotype and genotype are referred to as msd2. These alterations result in increased number of seeds and seed weight, thus increasing the yield of the genetically altered plant. These alterations can be generated in the ortholog genes in maize (TS1), rice, barley, and other monocot plants, generating the MSD2 phenotype. The seeds of one particular MSD2 Sorghum bicolor has been deposited with ATCC and assigned Accession Number PTA-121634.

公开(公告)号：US20190071666A1

公开(公告)日：2019-03-07

申请号：US16091913

申请日：2017-04-05

Applicant: Cold Spring Harbor Laboratory

Inventor： Anthony Zador ,  Justus Kebschull

IPC: C12N15/10 ,  C07K14/18 ,  C12N5/0793 ,  C40B70/00

Abstract: The present invention provides a composition comprising a plurality of labeled neurons, each of which is labeled by an expression construct that encodes a unique barcoded nucleic acid.

公开(公告)号：US20190030058A1

公开(公告)日：2019-01-31

申请号：US15892560

申请日：2018-02-09

Applicant: Biogen MA Inc. ,  Cold Spring Harbor Laboratory

Inventor： C. Frank Bennett ,  Gene Hung ,  Frank Rigo ,  Adrian R. Krainer ,  Yimin Hua ,  Marco A. Passini ,  Lamya Shihabuddin ,  Seng H. Cheng ,  Katherine W. Klinger

IPC: A61K31/7088 ,  C12N15/113 ,  A61K31/712

Abstract: Disclosed herein are compounds, compositions and methods for modulating splicing of SMN2 mRNA in a subject. Also provided are uses of disclosed compounds and compositions in the manufacture of a medicament for treatment of diseases and disorders, including spinal muscular atrophy.

公开(公告)号：US20180362987A1

公开(公告)日：2018-12-20

申请号：US15949902

申请日：2018-04-10

Applicant: Cold Spring Harbor Laboratory

Inventor： Adrian Krainer ,  Isabel Aznarez

IPC: C12N15/113 ,  C07K14/47 ,  C07K14/805 ,  C12N15/11 ,  C12Q1/6883

Abstract: Provided herein are methods and compositions for increasing production of a target protein or functional RNA by a cell.

公开(公告)号：US20180023139A1

公开(公告)日：2018-01-25

申请号：US15546106

申请日：2016-01-26

Applicant: Cold Spring Harbor Laboratory

Inventor： Christopher H. Vakoc ,  Junwei Shi ,  Justin B. Kinney

IPC: C12Q1/68 ,  G01N33/68

CPC classification number: C12Q1/6876 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/63 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2320/12 ,  C12N2330/31 ,  C12Q1/6897 ,  C12Q2600/156 ,  C12Q2600/178 ,  G01N33/68 ,  G01N33/6803

Abstract: Provided herein, in some aspects, are methods of determining whether a candidate protein, more specifically a functional domain of a candidate protein, is essential for viability of cells of interest using clustered regularly interspaced short palindromic repeat (CPJSPR)-Cas9 technology which holds great promise for genetic screening and for the discovery of therapeutic targets.

公开(公告)号：US20170306392A1

公开(公告)日：2017-10-26

申请号：US15515913

申请日：2015-10-09

Applicant: Cold Spring Harbor Laboratory

Inventor： Michael Wigler ,  Dan Levy

IPC: C12Q1/68 ,  C07H21/04 ,  C12N15/10

CPC classification number: C12Q1/6827 ,  C07H21/04 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/1058 ,  C12Q1/6851 ,  C12Q1/6869 ,  C12Q2521/539 ,  C12Q2525/161 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2545/114

Abstract: A method for determining the number of nucleic acid molecules (NAMs) in a group of NAMs, comprising i) obtaining an amplified and mutagenized group of NAMs that was produced by a. subjecting the group of NAMs to a chemical mutagenesis which mutates only select nucleic acid bases in the group of NAMs at a rate of 10% to 90% thus forming a group of mutagenized NAMs (mNAMs), and b. amplifying the group of mNAMs; ii) obtaining sequences of the mNAMs in the group of amplified mNAMs; and iii) counting the number of different sequences obtained in step (ii) to determine the number of unique mNAMs in the group of mNAMS, thereby determining the number of NAMs in the group of NAMs.

公开(公告)号：US20160298121A1

公开(公告)日：2016-10-13

申请号：US14874420

申请日：2015-10-03

Applicant: Cold Spring Harbor Laboratory

Inventor： Adrian Krainer ,  Isabel Aznarez

IPC: C12N15/113 ,  C12Q1/68

CPC classification number: C12N15/1137 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/4736 ,  C07K14/805 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/3341 ,  C12N2320/11 ,  C12N2320/33 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  C12Y101/01205 ,  C12Y304/24087 ,  C12N2310/321 ,  C12N2310/3521

Abstract: Provided herein are methods and compositions for increasing production of a target protein or functional RNA by a cell.

Abstract translation: 本文提供了用于增加细胞产生靶蛋白或功能性RNA的方法和组合物。

公开(公告)号：US20160266127A1

公开(公告)日：2016-09-15

申请号：US15024483

申请日：2014-09-30

Applicant: Peter KUHN ,  Angel Ernesto DAGO RODRIGUEZ ,  Anders CARLSSON ,  Wei LIU ,  Jim HICKS ,  THE SCRIPPS RESEARCH INSTITUTE ,  COLD SPRING HARBOR LABORATORY

Inventor： Peter Kuhn ,  Angel Ernesto Dago Rodriguez ,  Anders Carlsson ,  Wei Liu ,  Jim Hicks

IPC: G01N33/574

CPC classification number: G01N33/57434 ,  G01N33/57488 ,  G01N33/57492 ,  G01N2800/52

Abstract: The present invention provides methods for predicting response to a hormone-directed therapy or chemotherapy in a prostate cancer (PCa) patient comprising (a) performing a direct analysis comprising immunofluorescent staining and morphological characterization of nucleated cells in a blood sample obtained from the patient to identify and enumerate circulating tumor cells (CTC); (b) individually characterizing genotypic, morphometric and protein expression parameters to generate a profile for each of the CTCs, and (c) predicting response to hormone-directed therapy in the prostate cancer PCa patient based on said profile. In some embodiments, the methods comprise repeating steps (a) through (c) at one or more timepoints after initial diagnosis of prostate cancer to sequentially monitor said genotypic, morphometric and protein expression parameters.

Abstract translation: 本发明提供了用于预测前列腺癌（PCa）患者中激素指导性治疗或化学疗法的应答的方法，包括（a）进行直接分析，其包括从患者获得的血液样品中的有核细胞的免疫荧光染色和形态学表征， 识别和列举循环肿瘤细胞（CTC）; （b）单独表征基因型，形态测定和蛋白质表达参数以产生每个CTC的谱，以及（c）基于所述谱，预测前列腺癌PCa患者中激素指导性治疗的反应。 在一些实施方案中，所述方法包括在初步诊断前列腺癌之后的一个或多个时间点重复步骤（a）至（c），以顺序监测所述基因型，形态学和蛋白质表达参数。