公开(公告)号：US20240302334A1

公开(公告)日：2024-09-12

申请号：US18599970

申请日：2024-03-08

Applicant: Waters Technologies Corporation

Inventor： Jinchuan Yang ,  Paul Rainville

IPC: G01N30/56 ,  G01N30/04 ,  G01N30/06 ,  G01N30/72 ,  G01N30/74

CPC classification number: G01N30/56 ,  G01N30/06 ,  G01N30/7233 ,  G01N30/74 ,  G01N2030/045 ,  G01N2030/567

Abstract: The present disclosure is directed to methods of separating one or more non-modified oligosaccharide(s) with degree of polymerization 6 or higher from a sample using a hydrophilic interaction liquid chromatography (HILIC) column connected to a liquid chromatography system that includes coated flow paths. The methods disclosed herein requires, inter alia, a liquid chromatography system, wherein at least one component of the LC system includes a fluid-contacting alkylsilyl coating. When implementing a standard HILIC method, the methods of the present technology can be used to decrease the analyte loss and/or carry-over of the separation leading to improved analysis.

公开(公告)号：US12083498B2

公开(公告)日：2024-09-10

申请号：US16926102

申请日：2020-07-10

Applicant: Waters Technologies Corporation

Inventor： Darryl W. Brousmiche ,  Jacob N. Fairchild ,  Jason F. Hill ,  Giorgis Isaac ,  Michael F. Morris ,  Kevin D. Wyndham

IPC: B01D15/26 ,  B01D15/30 ,  B01D15/32 ,  B01D15/40 ,  B01D53/02 ,  B01J20/22 ,  B01J20/28 ,  B01J20/282 ,  B01J20/283 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/30 ,  B01J20/32 ,  C07F7/18

CPC classification number: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  C07F7/1804 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/32 ,  B01J20/3234 ,  B01J2220/54 ,  B01J2220/80

Abstract: The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. In some examples, the present disclosure can include a chromatographic system having a chromatographic column having a stationary phase with a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or a combination thereof.

公开(公告)号：US12078621B2

公开(公告)日：2024-09-03

申请号：US17699775

申请日：2022-03-21

Applicant: Waters Technologies Corporation

Inventor： Fabrice Gritti

IPC: G01N30/46 ,  G01N30/02 ,  G01N30/32 ,  G01N30/82 ,  G01N30/30

CPC classification number: G01N30/461 ,  G01N30/82 ,  G01N2030/027 ,  G01N2030/3007 ,  G01N2030/326 ,  G01N2030/328

Abstract: The disclosure relates to a gradient twin column recycling chromatography method that is used to separate a mixture containing closely eluting compounds. In one embodiment, a sample includes a primary organic compound and one or more impurities that closely elute with the primary organic compound. A gradient mobile phase is initially used to remove unwanted early eluting and late eluting impurities from the sample. After the gradient removal of some of the impurities is complete, the remaining mixture of the primary organic compound and the closely eluting impurities are separated using recycle chromatography methodology with an isocratic mobile phase.

公开(公告)号：US12007370B2

公开(公告)日：2024-06-11

申请号：US17347807

申请日：2021-06-15

Applicant: Waters Technologies Corporation

Inventor： Michael O. Fogwill ,  Wade P. Leveille ,  Joseph D. Michienzi ,  Fabrice Gritti

IPC: G01N30/30 ,  G01N30/02

CPC classification number: G01N30/30 ,  G01N2030/025 ,  G01N2030/027 ,  G01N2030/3015 ,  G01N2030/3046

Abstract: Exemplary embodiments may compensate for expected frictional heating or Joule-Thomson cooling in chromatography columns. Frictional heating or Joule Thomson cooling are the same thing for a fluid decompressing along a porous material. Either heat is absorbed from or released to the external environment. The exemplary embodiments may cool the mobile phase to a sub-ambient temperature before the mobile phase passes through a chromatography column to compensate for the frictional heating or heat the mobile phase to a super-ambient temperature to compensate for Joule-Thomson cooling. The amount of temperature increase expected from the frictional heating or the amount of temperature decrease expected from the Joule-Thomson cooling may be calculated or estimated. Based on the amount of temperature increase or decrease expected, the set point for the heater/cooler may be determined and applied to the mobile phase. The analyte may be injected solely into a central portion of the chromatography column to further compensate for thermal gradients.

公开(公告)号：US12000806B2

公开(公告)日：2024-06-04

申请号：US17733218

申请日：2022-04-29

Applicant: Waters Technologies Corporation

Inventor： Sylvain Gilles Cormier ,  Charles H. Phoebe, Jr.

IPC: G01N30/20 ,  B01D15/08 ,  G01N30/46 ,  G01N30/02 ,  G01N30/34

CPC classification number: G01N30/20 ,  B01D15/08 ,  G01N30/463 ,  G01N2030/027 ,  G01N30/34

Abstract: Described are an interface module for two-dimensional chromatography and a method of performing a chromatographic separation that may use the interface module. The interface module includes a valve module, a collection needle, a modifier module and a sample storage element. The valve module has a first port configured to receive an eluent from a first chromatography system, a second port configured to provide a fraction obtained from the eluent, a third port and a fourth port. The collection needle and the modifier module are in fluidic communication with the valve module at the third and fourth ports, respectively. The modifier module includes a source of a modifier solvent. The sample storage element is in fluidic communication with the valve module and is configured to receive a volume of the fraction for injection as a sample into a second chromatography system.

公开(公告)号：US11994500B2

公开(公告)日：2024-05-28

申请号：US17084998

申请日：2020-10-30

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Mark J. Basile ,  Jeffrey Musacchio ,  Michael O. Fogwill ,  Sebastien Besner ,  Joseph D. Michienzi ,  Wade P. Leveille

IPC: G01N30/74 ,  G01N30/30 ,  G01N30/02

CPC classification number: G01N30/74 ,  G01N30/30 ,  G01N2030/027 ,  G01N2030/3038 ,  G01N2030/746

Abstract: Techniques and apparatus for thermally controlled interfaces for separation devices and optical flow cell devices with minimized post-column volumes are described. In one embodiment, for example, a column-optical cell assembly may include an insulating device, a chromatography column arranged within the insulating device, and an optical flow cell in fluid communication with the chromatography column, the chromatography column arranged within a minimum distance of the optical flow cell.

公开(公告)号：US11986751B2

公开(公告)日：2024-05-21

申请号：US17241480

申请日：2021-04-27

Applicant: Waters Technologies Corporation

Inventor： Jonathan L. Belanger ,  Wade P. Leveille, Sr. ,  Michael O. Fogwill ,  Joseph D. Michienzi ,  Jeffrey Musacchio

IPC: B01D15/22 ,  B01D15/10 ,  F16J15/34 ,  G01N30/60

CPC classification number: B01D15/22 ,  B01D15/10 ,  F16J15/34 ,  G01N30/60 ,  G01N30/6004 ,  G01N30/6026

Abstract: Described are apparatuses, devices, and systems implementing seals for separation devices. An apparatus for a separation device may include an end nut shaped to be positioned on one of an inlet side and an outlet side of the separation device. The end nut may be further shaped to mate with a connecting fitting. The apparatus may further include a face seal having a device side and a fitting side. The face seal may be positioned, at least in part, in the end nut on the device side of the face seal. The face seal may be positioned to interface with the connecting fitting on the fitting side of the face seal upon the end nut being mated with the connecting fitting. The apparatus may also include a face seal bore of the face seal being axially alignable with at least one of an inlet and an outlet of the separation device.

公开(公告)号：US11951475B2

公开(公告)日：2024-04-09

申请号：US17325830

申请日：2021-05-20

Applicant: Waters Technologies Corporation

Inventor： Sylvain Gilles Cormier ,  Charles T. Murphy ,  Joseph D. Michienzi

IPC: B01L3/00 ,  F16K99/00 ,  G01N30/02

CPC classification number: B01L3/502715 ,  F16K99/0013 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2400/0415 ,  B01L2400/0487 ,  B01L2400/084 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N2030/027

Abstract: Described is a multi-channel fluidic device that includes a diffusion-bonded body having a device surface and a plurality of fluid channels. Each fluid channel includes a channel segment defined in a plane that is parallel to the device surface and parallel to each of the planes of the other channel segments. The plane of each channel segment is at a depth below the device surface that is different from the depth below the device surface for the other planes. Each channel segment may have a volume equal to the volume of each of the other channel segments. One of the fluid channels may include a plurality of channel segments serially connected to each other and each defined in a plane that is different from the planes of the other channel segments.

公开(公告)号：US11940429B2

公开(公告)日：2024-03-26

申请号：US16400163

申请日：2019-05-01

Applicant: Waters Technologies Corporation

Inventor： Jinchuan Yang ,  Paul Rainville

IPC: G01N30/86 ,  G01J3/00 ,  G01N30/02 ,  G01N30/72 ,  G01N30/74 ,  G01N33/00 ,  G01R33/46

CPC classification number: G01N30/8675 ,  G01J3/00 ,  G01N30/72 ,  G01N30/74 ,  G01N33/0098 ,  G01R33/46 ,  G01N2030/025 ,  G01N2030/027

Abstract: A method is provided for authenticating a botanical. The method includes identifying at least one marker compound of the botanical. A sample comprising the botanical is injected into a chromatography system that includes a mobile phase delivery module, an autosampler, a chromatography column, a chromatography column manager, and at least one detector. The method also includes extracting a chromatogram of the at least one marker compound and extracting a spectrum at a chromatographic peak retention time of the at least one marker compound. The method also includes quantifying at least one peak of the extracted chromatogram and quantifying at least one band of the extracted spectrum. The quantified at least one peak of the extracted chromatogram and the quantified at least one band of the extracted spectrum are compared to a set of authentication criteria to determine authenticity of the botanical.

公开(公告)号：US20240092959A1

公开(公告)日：2024-03-21

申请号：US18174309

申请日：2023-02-24

Applicant: Waters Technologies Corporation

Inventor： Nicole L. Lawrence ,  Peng Chen ,  Mingcheng Xu ,  Daniel P. Walsh ,  Matthew A. Lauber

IPC: C08F283/06

CPC classification number: C08F283/06

Abstract: The present disclosure pertains to non-porous composite particles that are non-porous, polymer-based particles. In various embodiments, a non-porous polymer core is surface modified. In various embodiments, a non-porous hybrid organic-inorganic material is in contact with the modified surface of the core, and a bonding material is in contact with the non-porous hybrid organic-inorganic material. The present disclosure pertains to chromatographic separation devices that comprise such non-porous composite particles.