公开(公告)号：US20190234931A1

公开(公告)日：2019-08-01

申请号：US16383427

申请日：2019-04-12

Applicant: Instrumentation Laboratory Company

Inventor： Ethan Schonbrun ,  Gert Blankenstein ,  Josef Kerimo ,  Hansong Zeng

IPC: G01N33/49 ,  G01N21/25 ,  G01N21/05 ,  G01N21/552 ,  G01N21/03 ,  B01L3/00

CPC classification number: G01N33/49 ,  B01L3/502 ,  B01L2300/0654 ,  B01L2300/168 ,  G01N21/0303 ,  G01N21/05 ,  G01N21/25 ,  G01N21/552 ,  G01N2201/0612

Abstract: Analyte content in a cell free portion of a body fluid, such as blood, is optically determined without centrifugation or other preliminary steps for separating the cell free portion from the body fluid. A channel is configured for containing a flowing sample of the body fluid along an optical boundary. The channel is configured so that a cell free layer of the fluid naturally forms along the boundary of the channel which coincides with the optical boundary. A light source is directed onto the optical boundary at an angle selected to generate total reflection from the boundary and to generate an evanescent field across the boundary in the cell free layer of fluid. A light detector is configured to detect absorption of the light in the evanescent field. The light source and light detector are matched to the wavelength range of an absorption peak of the analyte being detected.

公开(公告)号：US10168278B2

公开(公告)日：2019-01-01

申请号：US15725504

申请日：2017-10-05

Applicant: Instrumentation Laboratory Company

Inventor： Ethan Schonbrun ,  Lara Adib ,  Gert Blankenstein

IPC: G01N21/41 ,  G01N33/49 ,  G01N21/27 ,  G01N33/68

Abstract: An optical system and method for quantifying total protein in whole blood or other multi-phase liquids and colloidal suspensions uses refractometry without preliminary steps such as cell separation or centrifugation. A refractometer is integrated with a flow cell to enable the refractive index of a flowing sample to be measured based on a substantially cell free boundary layer of the sample that is present under certain flow conditions. Dimensions of the flow cell are selected to produce a cell-free layer in a flow of whole blood in which the cell free layer is thick enough to reduce scattering of light from the refractometer light source. A numerical method is used to compensate for scattering artifacts. The numerical compensation method is based on the slope and width of a peak in the derivative curve of an angular spectrum image of the flowing sample produced by refractometry.