Detection of microorganisms with a fluorescence-based device

    公开(公告)号:US09207180B2

    公开(公告)日:2015-12-08

    申请号:US13068660

    申请日:2011-05-17

    Applicant: Gideon Eden

    Inventor: Gideon Eden

    CPC classification number: G01N21/6486 C12Q1/04 C12Q1/06 G01N21/6428 G01N33/582

    Abstract: A device and method for detecting by fluorescence microbial growth from sample substances are disclosed. For example, a method for the detection of visible-band fluorescence signals generated by at least one fluorescing compound excited by ultraviolet energy, comprising exciting said at least one fluorescing compound with ultraviolet energy emitted from a light-emitting diode comprising wavelengths below 400 nanometers, and detecting a visible-band fluorescence signal generated by said at least one excited fluorescing compound with at least one light detector sensitive to electromagnetic energy comprising wavelengths greater than or equal to 400 nanometers wavelength. For example, a device for detecting visible-band fluorescence signals generated by at least one fluorescing compound excited by ultraviolet energy, comprising at least one ultraviolet light-emitting diode generating electromagnetic radiation comprising wavelengths below 400 nanometers and capable of exciting the at least one fluorescing compound, at least one light detector sensitive to electromagnetic energy comprising wavelengths greater than or equal to 400 nanometers wavelength for the detection of visible-band fluorescence signals generated by the at least one fluorescing compound.

    Optical method and device for the detection and enumeration of microorganisms
    12.
    发明授权
    Optical method and device for the detection and enumeration of microorganisms 有权
    用于微生物检测和计数的光学方法和装置

    公开(公告)号:US08557539B2

    公开(公告)日:2013-10-15

    申请号:US11766223

    申请日:2007-06-21

    CPC classification number: C12Q1/06 C12M23/34 C12M41/36 C12Q1/04

    Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device comprises a container with at least one section transparent to light, a growth zone located in said container containing a mixture of growth media capable of supporting growth of the microorganisms, and at least one indicator substrate that changes its optical properties due to growth of the microorganisms. A detection zone is located in the container adjacent to the transparent section, and a barrier layer comprising porous solid material separates the two zones, allowing diffusion of molecules and ions of metabolic by-products of the organisms, while preventing microorganisms and particulate matter of the test sample from penetrating into the detection zone.

    Abstract translation: 描述了用于检测测试样品中活的微生物的存在的新的装置和方法。 该装置包括具有至少一个对光透明的部分的容器,位于所述容器中的生长区,其包含能够支持微生物生长的生长培养基的混合物,以及至少一个指示剂基质,其由于生长而改变其光学性质 微生物。 检测区域位于与透明部分相邻的容器中,并且包含多孔固体材料的阻挡层分离两个区域,从而允许生物体的代谢副产物的分子和离子的扩散,同时防止微生物和微生物的微生物和颗粒物质 测试样品渗入检测区。

    Detection of microorganisms with a fluorescence-based device

    公开(公告)号:US08535937B2

    公开(公告)日:2013-09-17

    申请号:US13068639

    申请日:2011-05-17

    Applicant: Gideon Eden

    Inventor: Gideon Eden

    CPC classification number: G01N21/6486 C12Q1/04 C12Q1/06 G01N21/6428 G01N33/582

    Abstract: A device and method for detecting by fluorescence microbial growth from sample substances are disclosed. For example, a method for the detection of visible-band fluorescence signals generated by at least one fluorescing compound excited by ultraviolet energy, comprising exciting said at least one fluorescing compound with ultraviolet energy emitted from a light-emitting diode comprising wavelengths below 400 nanometers, and detecting a visible-band fluorescence signal generated by said at least one excited fluorescing compound with at least one light detector sensitive to electromagnetic energy comprising wavelengths greater than or equal to 400 nanometers wavelength. For example, a device for detecting visible-band fluorescence signals generated by at least one fluorescing compound excited by ultraviolet energy, comprising at least one ultraviolet light-emitting diode generating electromagnetic radiation comprising wavelengths below 400 nanometers and capable of exciting the at least one fluorescing compound, at least one light detector sensitive to electromagnetic energy comprising wavelengths greater than or equal to 400 nanometers wavelength for the detection of visible-band fluorescence signals generated by the at least one fluorescing compound.

    CO2 OPTICAL SENSOR FOR DETECTION AND ENUMERATION OF MICROORGANISMS
    14.
    发明申请
    CO2 OPTICAL SENSOR FOR DETECTION AND ENUMERATION OF MICROORGANISMS 有权
    用于检测和产生微生物的二氧化碳光学传感器

    公开(公告)号:US20100273209A1

    公开(公告)日:2010-10-28

    申请号:US12740078

    申请日:2008-01-17

    Abstract: A new device and method for detecting the presence of living microorganisms in test samples are described. The device includes a container having at least one section transparent to light with an incubation zone defined in the container, the incubation zone containing growth media in which the sample is cultured. A detection zone containing a matrix composed of a polymeric material which is substantially transparent to light, and at least one indicator reagent sensitive to carbon dioxide gas generated by the microorganisms in the incubation zone is located in the transparent section of the matrix. The matrix is configured to facilitate penetration of external light aimed at the transparent section of the container and interaction of the external light with the indicator reagent to yield interactive light that escapes through the transparent section of the container, said interactive light is being indicative of the presence and/or concentration of the microorganisms.

    Abstract translation: 描述了用于检测测试样品中活的微生物的存在的新的装置和方法。 该装置包括容器,该容器具有至少一个对容器透明的部分和孵育区域的透明部分,孵育区域包含其中培养样品的生长培养基。 含有由对光基本上透明的聚合物材料构成的基质的检测区域以及由温育区域中的微生物产生的对二氧化碳气体敏感的至少一种指示剂试剂位于基质的透明部分。 该矩阵被配置成便于穿透容器的透明部分的外部光和外部光与指示剂试剂的相互作用,以产生通过容器的透明部分逸出的交互光,所述交互光指示 微生物的存在和/或浓度。

    Method of Making a Test Device
    15.
    发明申请
    Method of Making a Test Device 有权
    制造测试装置的方法

    公开(公告)号:US20080305013A1

    公开(公告)日:2008-12-11

    申请号:US11758327

    申请日:2007-06-05

    Applicant: Gideon Eden

    Inventor: Gideon Eden

    Abstract: A method of making a test device comprised of a container divided into two chambers, each holding a volume of a clear test liquid, the chambers separated by a porous barrier. The liquid is introduced into the lower chamber by adding a liquid to the upper chamber through the open top of the container and then applying a vacuum to completely evacuate air from the lower chamber by drawing the air through the barrier and liquid in the upper chamber. Restoring air pressure forces liquid in the upper chamber into the lower chamber, to completely fill the same while leaving a volume of liquid in the upper chamber. The application of a vacuum is preferably carried out by placing the container with liquid in a receptacle containing a liquid to be boiled, heating the liquid to boil the same and thereafter condensing the vapor with the receptacle sealed to develop a very high vacuum which draws the air out from the lower chamber of the container. The boiling can be done with the receptacle sealed to develop a higher temperature and later vented prior to being resealed for cooling and condensing of the vaporized liquid to further enhance the level of vacuum achieved.

    Abstract translation: 一种制造测试装置的方法,该测试装置由分成两个室的容器组成,每个容器容纳一个清晰的测试液体,该室由多孔屏障隔开。 通过将液体通过容器的开口顶部向上部腔室中加入液体,然后通过抽吸空气通过上部腔室中的屏障和液体来施加真空,从而将空气从下部腔室中完全排出,从而将液体引入下部腔室。 恢复空气压力迫使上部室中的液体进入下部室,以完全填充它,同时在上部室中留下一定体积的液体。 真空的施加优选通过将容器放置在含有待煮沸液体的容器中,加热液体以使其沸腾,然后将蒸气与容器密封以形成非常高的真空度来进行,以吸收 从容器的下腔排出空气。 沸腾可以通过密封的容器进行,以开发更高的温度,然后在被再密封之前排出以冷却和冷凝汽化液体,以进一步提高所获得的真空度。

    Device and method for use in detecting microorganisms in a sample
    16.
    发明授权
    Device and method for use in detecting microorganisms in a sample 失效
    用于检测样品中微生物的装置和方法

    公开(公告)号:US5366873A

    公开(公告)日:1994-11-22

    申请号:US892581

    申请日:1992-06-03

    CPC classification number: C12Q1/04 C12M41/36 C12M41/46 Y10S435/808

    Abstract: A new device and method to detect the presence of live microorganisms are described. The device employs a liquid phase which supports microbial growth by utilizing appropriate media. A secondary phase which consists of semi-liquid material forms a barrier layer through which only small particles can diffuse and which prevent the presence of any large particles. Noise-free optical measurements are carried out at the barrier layer which are indicative of metabolic changes associated with microbial growth.

    Abstract translation: 描述了检测活微生物存在的新装置和方法。 该装置采用通过利用适当介质来支持微生物生长的液相。 由半液体材料组成的次相形成阻挡层,只有小颗粒才能扩散并阻止任何大颗粒的存在。 在阻挡层进行无噪声的光学测量,其指示与微生物生长相关的代谢变化。

    Diagnostic microbiological testing apparatus and method
    17.
    发明授权
    Diagnostic microbiological testing apparatus and method 失效
    诊断微生物检测仪器及方法

    公开(公告)号:US5340747A

    公开(公告)日:1994-08-23

    申请号:US72346

    申请日:1993-06-04

    Applicant: Gideon Eden

    Inventor: Gideon Eden

    Abstract: A diagnostic microbiological testing apparatus and method includes at least one test tray including a plurality of reaction chambers, a light source disposed proximate to the test tray for directing light, at an excitation wavelength of a fluorescence emitting agent contained within the reaction chambers, at the test tray, a filter for passing therethrough only light generated by a fluorescence emitting reaction resulting from the interaction of the fluorescence emitting agent and a sample, and an imaging mechanism for detecting only the light generated by the fluorescence emitting reaction at the emission wavelength simultaneously from the plurality of reaction chambers.

    Abstract translation: 一种诊断微生物测试装置和方法包括至少一个包括多个反应室的测试托盘,靠近测试托盘设置的光源,用于在包含在反应室内的荧光发射剂的激发波长处引导光 测试托盘,用于仅通过由荧光发射剂和样品的相互作用产生的荧光发射反应产生的光的滤光器,以及用于仅从发光波长的荧光发射反应产生的光同时从 多个反应室。

    System for fast battery charging
    18.
    发明授权

    公开(公告)号:US12024045B1

    公开(公告)日:2024-07-02

    申请号:US18525737

    申请日:2023-11-30

    Applicant: Gideon Eden

    Inventor: Gideon Eden

    Abstract: A battery is provided that uses supercapacitors and battery cells connected by switches among themselves and to input and output terminals via multiplexed selection under the control of a microprocessor or microcontroller. The supercapacitors and battery cells of the battery may independently, or in combination, power an electric vehicle. The battery may be operated in several modes, at least one of which may be used to power an electric vehicle long enough to reach a charging station in connection with charge on the battery cells being partially or almost completely depleted. The battery may also deliver charge while the battery cells and/or the supercapacitors are being charged. The battery may be fast charging on-the-run in that the supercapacitors may be charged faster than the battery cells permitting an electric vehicle to be powered without spending considerable time, that would otherwise be required at a conventional charging station with traditional batteries.

    PROCESSING VIDEO AND AUDIO STREAMING DATA
    19.
    发明申请

    公开(公告)号:US20200329273A1

    公开(公告)日:2020-10-15

    申请号:US16915741

    申请日:2020-06-29

    Applicant: Gideon Eden

    Inventor: Gideon Eden

    Abstract: The disclosure includes a method of generating and presenting non-processed streaming audio data and non-processed streaming video data to a first viewer and a second viewer. The method may include presenting, via a commercial television display unit, the non-processed streaming video data to at least one of the commentator, the first viewer, and the second viewer. The method may include generating the non-processed streaming audio data related to the live event by the commentator, and transferring the non-processed streaming audio data via an Internet to the first viewer and the second viewer. Also, the method may include providing the first viewer and the second viewer with a capability to synchronize a video presentation time of the non-processed streaming video data with an audio presentation time of the non-processed streaming audio data.

    Processing video and audio streaming data

    公开(公告)号:US10750208B2

    公开(公告)日:2020-08-18

    申请号:US16234560

    申请日:2018-12-28

    Applicant: Gideon Eden

    Inventor: Gideon Eden

    Abstract: The disclosure includes a method of generating and presenting digital streaming data to a viewer. The digital streaming data comprises streaming video data depicted from a live event and streaming audio data generated by a commentator, the streaming audio data related to the live event. In many embodiments, the method includes presenting the streaming video data to the commentator and the viewer and generating streaming audio data by the commentator related to the live event. Methods may also include transferring the streaming audio data via an Internet to the viewer and providing the viewer with synchronization means to synchronize the streaming video data with the streaming audio data.

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