公开(公告)号：US20230030220A1

公开(公告)日：2023-02-02

申请号：US17757646

申请日：2020-12-18

Applicant: Glycom A/S

Inventor： Nikolay KHANZHIN ,  Getachew S. MOLLA ,  Martin MATWIEJUK ,  Pierre CHASSAGNE

IPC: C12P19/04 ,  B01D61/14 ,  B01D61/58 ,  B01D69/02 ,  B01D71/56 ,  C12P19/26

Abstract: The present invention relates to the separation and isolation of sialylated human milk oligosaccharides (HMOs) from the reaction mixture in which they are produced.

公开(公告)号：US20220395520A1

公开(公告)日：2022-12-15

申请号：US17821282

申请日：2022-08-22

Applicant: GLYCOM A/S

Inventor： Nikolay KHANZHIN ,  Markus JONDELIUS HEDEROS ,  Louise Kristine VIGSNÆS ,  Bruce MCCONNELL

IPC: A61K31/702 ,  C12P19/04 ,  C12P1/04 ,  C12N9/10 ,  A61K31/716 ,  A61P31/04 ,  A61K31/7016 ,  A61K31/7032 ,  C07H1/00

Abstract: A mixture of human milk oligosaccharides that comprises or consists essentially of a) LNTri II, LNT, pLNH II and optionally lactose, or b) LNTri II, LNnT, pLNnH and optionally lactose, and is useful for preventing or treating viral or bacterial, intestinal and respiratory infections and for modulating the human gastrointestinal microbiota to increase Bifidobacterium abundance. A process for preparing the mixture and methods of crystallizing LNnT and LNT is also described.

公开(公告)号：US20200148793A1

公开(公告)日：2020-05-14

申请号：US16626122

申请日：2018-06-27

Applicant: GLYCOM A/S

Inventor： Nikolay KHANZHIN ,  Markus JONDELIUS HEDEROS

IPC: C08B37/00 ,  C12P19/04 ,  B01D69/12 ,  B01D69/02 ,  C12P19/12 ,  B01D61/02 ,  B01D71/56

Abstract: The invention relates to separation of disaccharides from tri- or higher oligosaccharides by nanofiltration.

公开(公告)号：US20190175631A1

公开(公告)日：2019-06-13

申请号：US16312506

申请日：2017-06-23

Applicant: GLYCOM A/S

Inventor： Nikolay KHANZHIN ,  Markus JONDELIUS HEDEROS ,  Louise Kristine VIGSNÆS ,  Bruce MCCONNELL

IPC: A61K31/702 ,  A61K31/7032 ,  A61K31/7016 ,  A61P31/04 ,  C07H1/00

Abstract: A mixture of human milk oligosaccharides that comprises or consists essentially of a) LNTri II, LNT, pLNH II and optionally lactose, or b) LNTri II, LNnT, pLNnH and optionally lactose, and is useful for preventing or treating viral or bacterial, intestinal and respiratory infections and for modulating the human gastrointestinal microbiota to increase Bifidobacterium abundance. A process for preparing the mixture and methods of crystallizing LNnT and LNT is also described.

公开(公告)号：US20190119314A1

公开(公告)日：2019-04-25

申请号：US16094508

申请日：2017-04-19

Applicant: GLYCOM A/S

Inventor： Pierre CHASSAGNE ,  Nikolay KHANZHIN ,  Martin MATWIEJUK ,  Markus JONDELIUS HEDEROS ,  Niels BANKE

IPC: C07H13/04 ,  C07H1/06 ,  B01D61/02 ,  B01D61/14 ,  C12P19/28 ,  B01D61/58

Abstract: The invention relates to a method for obtaining an N-acetylglucosamine containing neutral oligosaccharide from a fermentation broth, wherein said oligosaccharide is produced by culturing a genetically modified microorganism capable of producing said oligosaccharide from an internalized carbohydrate precursor, comprising the steps of: i) ultrafiltration (UF), preferably to separate biomass from the broth, ii) nanofiltration (NF), preferably to concentrate said oligosaccharide in the broth and/or reduce an inorganic salt content of the broth, and iii) treating the broth with an ion exchange resin, preferably to remove charged materials, and/or subjecting the broth to chromatography, preferably to remove hydrophobic impurities.