公开(公告)号：US10174066B2

公开(公告)日：2019-01-08

申请号：US15480043

申请日：2017-04-05

Applicant: INTELLIGENT BIO-SYSTEMS, Inc.

Inventor： Steven Gordon ,  Jerzy Olejnik

IPC: C07H19/00 ,  G01N21/05 ,  G01N21/64 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/03

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

公开(公告)号：US10160995B2

公开(公告)日：2018-12-25

申请号：US14264758

申请日：2014-04-29

Applicant: Intelligent Bio-Systems, Inc

Inventor： Jerzy Olejnik ,  Steven Gordon ,  Martina Werner

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844

Abstract: Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification. A separation device is employed that can retain larger beads with bound amplified beads. The technique finds many uses, including enriching for beads with clonally amplified template, which can be used in a variety of assays, including nucleic acid sequencing.

公开(公告)号：US09909174B2

公开(公告)日：2018-03-06

申请号：US15214737

申请日：2016-07-20

Applicant: INTELLIGENT BIO-SYSTEMS, INC.

Inventor： Steven Gordon ,  Jerzy Olejnik

IPC: C12Q1/68 ,  C12P19/34 ,  G01N21/05 ,  G01N21/64 ,  G01N21/03

CPC classification number: C07H19/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2535/101 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2021/6417 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6471 ,  G01N2201/062 ,  G01N2201/0627 ,  G01N2201/12

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

公开(公告)号：US20140349887A1

公开(公告)日：2014-11-27

申请号：US14456525

申请日：2014-08-11

Applicant: Intelligent Bio-Systems, Inc.

Inventor： Steven Gordon ,  Daniel Stetson

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12N15/10 ,  C40B50/14 ,  G01N33/54353

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

Abstract translation: 本发明涉及用于扩增核酸的方法和装置，特别是扩增以在不使用乳剂的情况下在表面上产生产物。 在优选实施例中，在表面上产生多组放大产物，每组放置在所述表面上的不同（通常是预定的）位置，以便产生阵列。