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    Methods and compositions for incorporating nucleotides
    1.
    发明授权
    Methods and compositions for incorporating nucleotides 有权

    公开(公告)号：US10329611B2

    公开(公告)日：2019-06-25

    申请号：US15911801

    申请日：2018-03-05

    Applicant: INTELLIGENT BIO-SYSTEMS, INC.

    Inventor： Steven Gordon ,  Jerzy Olejnik

    IPC: C12Q1/68 ,  C12Q1/6869 ,  C07H19/00 ,  G01N21/05 ,  G01N21/64 ,  C12Q1/6874 ,  G01N21/03

    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

    Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
    4.
    发明授权
    Apparatus and methods for purging flow cells in nucleic acid sequencing instruments 有权
    用于清除核酸测序仪器中的流动细胞的装置和方法

    公开(公告)号：US09146248B2

    公开(公告)日：2015-09-29

    申请号：US13802957

    申请日：2013-03-14

    Applicant: INTELLIGENT BIO-SYSTEMS, INC.

    Inventor： Thomas Hagerott ,  Edmund W. Golaski

    IPC: G01N35/10 ,  B01L3/00

    CPC classification number: G01N35/1004 ,  B01L3/502707 ,  G01N35/085 ,  G01N35/10 ,  G01N35/1095 ,  Y10T29/49826

    Abstract: A method for operating a nucleic acid sequencing instrument with movable flow cells. The method includes providing a flow cell having a flow path with an inlet port and an outlet port and filling the flow path, from the inlet port to the outlet port, with a first liquid reagent. The method also includes providing a station block having an inlet passage and an outlet passage, mounting the flow cell on the station block with the inlet port in fluid communication with the inlet passage and the outlet port in fluid communication with the outlet passage, introducing a gaseous bubble into the inlet port, conveying a second liquid reagent from the inlet passage into the inlet port to move the gaseous bubble through the flow path and into the outlet passage and fill the flow passage with the second liquid reagent, and removing the flow cell from the station block.

    Abstract translation: 用于操作具有可移动流通池的核酸测序仪的方法。 该方法包括提供具有入口和出口的流路的流动池，并且从入口端口到出口端口用第一液体试剂填充流路。 该方法还包括提供具有入口通道和出口通道的站块，将流动池安装在站块上，入口与入口通道和出口端口流体连通，与出口通道流体连通，引入一个 将气泡进入入口，将第二液体试剂从入口通道输送到入口端口，以将气泡移动通过流动通道并进入出口通道并用第二液体试剂填充流动通道，并且移除流动池 从车站区块。

    Analyte Enrichment Methods And Compositions
    5.
    发明申请
    Analyte Enrichment Methods And Compositions 审中-公开
    分析物富集方法和组成

    公开(公告)号：US20140335528A1

    公开(公告)日：2014-11-13

    申请号：US14264758

    申请日：2014-04-29

    Applicant: Intelligent Bio-Systems, Inc

    Inventor： Jerzy Olejnik ,  Steven Gordon ,  Martina Werner

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/159 ,  C12Q2563/131 ,  C12Q2563/149 ,  C12Q2563/159

    Abstract: Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification. A separation device is employed that can retain larger beads with bound amplified beads. The technique finds many uses, including enriching for beads with clonally amplified template, which can be used in a variety of assays, including nucleic acid sequencing.

    Abstract translation: 公开了用于富集含有分析物的颗粒群的方法和组合物。 在一个实施方案中，使用比用于扩增的珠更大的富集珠。 使用分离装置，其可以用结合的扩增珠粒保留较大的珠粒。 该技术发现许多用途，包括用克隆扩增模板富集珠，可用于多种测定，包括核酸测序。

    Methods and compositions for incorporating nucleotides
    6.
    发明授权
    Methods and compositions for incorporating nucleotides 有权

    公开(公告)号：US10174066B2

    公开(公告)日：2019-01-08

    申请号：US15480043

    申请日：2017-04-05

    Applicant: INTELLIGENT BIO-SYSTEMS, Inc.

    Inventor： Steven Gordon ,  Jerzy Olejnik

    IPC: C07H19/00 ,  G01N21/05 ,  G01N21/64 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/03

    Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

    Analyte enrichment methods and compositions
    7.
    发明授权
    Analyte enrichment methods and compositions 有权

    公开(公告)号：US10160995B2

    公开(公告)日：2018-12-25

    申请号：US14264758

    申请日：2014-04-29

    Applicant: Intelligent Bio-Systems, Inc

    Inventor： Jerzy Olejnik ,  Steven Gordon ,  Martina Werner

    IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6844

    Abstract: Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification. A separation device is employed that can retain larger beads with bound amplified beads. The technique finds many uses, including enriching for beads with clonally amplified template, which can be used in a variety of assays, including nucleic acid sequencing.

    Flow cell alignment methods and systems
    9.
    发明授权
    Flow cell alignment methods and systems 有权

    公开(公告)号：US09554095B2

    公开(公告)日：2017-01-24

    申请号：US13832509

    申请日：2013-03-15

    Applicant: INTELLIGENT BIO-SYSTEMS, INC.

    Inventor： David Ray Stoops ,  Phillip Alan Veatch ,  Steven Jeffrey Gordon

    IPC: C12Q1/68 ,  G01N21/64 ,  H04N7/18 ,  G01N15/14 ,  G06T7/00 ,  G01N15/00

    Abstract: Imagers and alignment methods for use by imagers imaging deoxyribonucleic acid (DNA) fragments on a flow cell are disclosed. The imagers capture intensity values at DNA fragment bead locations in tiles with each tile having a reference location in the flow cell. Flow cells may be aligned by obtaining a dark field image of each tile during a first imaging session, identifying dark field constellations of bead locations within two separate tiles during the first imaging session, identifying corresponding constellations during a second imaging session, altering the reference location of at least one tile during the second imaging session to correct for a linear offset in the corresponding constellations, and applying at least one correction factor for reading out intensity values from the imager for the bead locations in the flow cell to correct for an angular offset determined from offsets in the corresponding constellations.

    Non-Emulsion Methods And Masked Biomolecules
    10.
    发明申请
    Non-Emulsion Methods And Masked Biomolecules 审中-公开
    非乳化方法和掩蔽生物分子

    公开(公告)号：US20140349887A1

    公开(公告)日：2014-11-27

    申请号：US14456525

    申请日：2014-08-11

    Applicant: Intelligent Bio-Systems, Inc.

    Inventor： Steven Gordon ,  Daniel Stetson

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/10 ,  C40B50/14 ,  G01N33/54353

    Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

    Abstract translation: 本发明涉及用于扩增核酸的方法和装置，特别是扩增以在不使用乳剂的情况下在表面上产生产物。 在优选实施例中，在表面上产生多组放大产物，每组放置在所述表面上的不同（通常是预定的）位置，以便产生阵列。

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