公开(公告)号：US20210053065A1

公开(公告)日：2021-02-25

申请号：US16998646

申请日：2020-08-20

Applicant: Pattern Bioscience, Inc.

Inventor： Nicolas Arab ,  Ross Johnson

IPC: B01L3/00 ,  C12M1/12 ,  C12M1/34 ,  C12Q1/04

Abstract: A method of analyzing a sample comprising one or more species of microorganisms can include generating first droplets such that each of one or more microorganisms of a first portion of the sample is encapsulated within one of the first droplets and, for each of one or more aliquots of a second portion of the sample, second droplets such that each of one or more microorganisms of the aliquot is encapsulated within one of the second droplets. First and second sets of data can be captured, the first set indicative of the identity and quantity of encapsulated microorganism(s) of the first portion of the sample and the second set indicative of a phenotypic response of encapsulated microorganism(s) of the aliquot(s) to one or more test reagents. A target species' phenotypic response to the test reagent(s) is determinable at least by referencing the second data set to the first data set.

公开(公告)号：US12036556B2

公开(公告)日：2024-07-16

申请号：US18045386

申请日：2022-10-10

Applicant: Pattern Bioscience, Inc.

Inventor： Ross Johnson

IPC: B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B23Q17/24 ,  C12Q1/18 ,  G01N21/64 ,  G01N33/50

CPC classification number: B01L3/502784 ,  B01F23/4105 ,  B01L3/502707 ,  B01L3/502746 ,  C12Q1/18 ,  G01N21/6452 ,  G01N33/5008 ,  B01F23/06 ,  B01F23/4143 ,  B01F23/4145 ,  B01F2101/23 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0861 ,  B01L2300/12 ,  B01L2400/02 ,  B01L2400/084 ,  B23Q17/249 ,  C12Q2563/159

Abstract: Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

公开(公告)号：US11959910B2

公开(公告)日：2024-04-16

申请号：US17372957

申请日：2021-07-12

Applicant: PATTERN BIOSCIENCE, INC.

Inventor： Nicolas Arab ,  Ross Johnson

IPC: C12Q1/04 ,  C12Q1/18 ,  G01N15/14 ,  G01N21/64 ,  G01N33/50 ,  G01N33/58 ,  G01N15/10 ,  G01N15/1404

CPC classification number: G01N33/5091 ,  C12Q1/04 ,  C12Q1/18 ,  G01N15/1463 ,  G01N21/6428 ,  G01N33/5011 ,  G01N33/582 ,  G01N2015/1006 ,  G01N2015/1406 ,  G01N2015/1481 ,  G01N2201/0621 ,  G01N2800/26

Abstract: Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.