公开(公告)号：US20230168230A1

公开(公告)日：2023-06-01

申请号：US18096477

申请日：2023-01-12

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Daniel Intelmann ,  Katrin Koenig ,  Patrick Neiens ,  Tibor Toth

IPC: G01N30/88 ,  G01N30/86

CPC classification number: G01N30/88 ,  G01N30/8658 ,  G01N2030/889

Abstract: The present invention relates to a method for operating a chromatography column comprising (a) providing a first value of a lifetime (first lifetime value) of said chromatography column; (b) performing a chromatographic separation of a sample on said chromatography column; (c) providing a value of a weighted aging factor determined based on at least one aging parameter selected from sample type, sample dilution, and sample volume; and (d) determining a second value of said lifetime (second lifetime value) of said chromatography column based on said first lifetime value and said weighted aging factor. The present invention also relates to further methods, databases, devices, and uses related thereto.

公开(公告)号：US20230079433A1

公开(公告)日：2023-03-16

申请号：US17992510

申请日：2022-11-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Daniel Intelmann

IPC: H01J49/00

Abstract: A computer implemented method for calibrating a customer mass spectrometry instrument (118) for quantifier-qualifier-ratio check is proposed. The method comprises the following steps: a) at least one manufacturer-site standardization, wherein a set of samples of a subject and a set of calibrator samples are measured in multiple replicates on a plurality of mass spectrometry instruments (114), wherein each measurement comprises multiple reaction monitoring with quantifier and qualifier transition for analyte and internal standard, wherein at least three adjustment factors are determined from the measurements of the set of samples of a subject and the set of calibrator samples, wherein a first adjustment factor α depends on a difference between analyte and internal standard, wherein a second adjustment factor β depends on a difference between samples of a subject and calibrator samples for analyte quantifier-qualifier-ratio, wherein a third adjustment factor γ depends on a difference between samples of a subject and calibrator samples for the internal standard quantifier-qualifier-ratio; b) at least one transfer step, wherein the adjustment factors are electronically transferred to a customer mass spectrometry instrument (118); c) at least one customer-site calibration, wherein the customer-site calibration comprises at least one calibration measurement, wherein a set of calibrator samples is measured on the customer mass spectrometry instrument (118) and quantifier-qualifier-ratios are determined therefrom, wherein target values for quantifier-qualifier-ratios for analyte and for internal standard are set by applying the adjustment factors on the determined quantifier-qualifier-ratios.

公开(公告)号：US20230003698A1

公开(公告)日：2023-01-05

申请号：US17931993

申请日：2022-09-14

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Daniel Intelmann

IPC: G01N30/86 ,  G01N30/72

Abstract: The present disclosure relates to a method for determining a carry over of an analyte from a previous sample into a sample of interest on a liquid chromatography mass spectrometer (LC-MS) device, the method comprising the following steps: (a) determining at least one chromatogram of said sample of interest on said LC-MS device; (b) determining a background height of the chromatogram; and (c) determining the carry over of the analyte from said previous sample into the sample of interest based on the background height. The present disclosure also relates to methods, systems, and computer program products related to the aforesaid method.

公开(公告)号：US20230003696A1

公开(公告)日：2023-01-05

申请号：US17930616

申请日：2022-09-08

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Daniel Intelmann ,  Julian Michely

IPC: G01N30/62

Abstract: A method for identifying and/or verifying at least one analyte peak in a chromatogram of a sample for said analyte from a liquid chromatography mass spectrometer device, said method comprising: a) determining a chromatogram of the sample by acquiring a plurality of data points for quantifier signal intensities and/or qualifier signal intensities, over time; and, in case the sample comprises an internal standard, optionally acquiring a plurality of data points for internal standard quantifier signal intensities and/or internal standard qualifier signal intensities, over time; b) determining for at least a fraction of the data points acquired in step a), a ratio type; c) comparing the ratios determined in step b) to a reference; and d) identifying and/or verifying at least one analyte peak in a chromatogram based on comparison step c).