公开(公告)号：US11692015B2

公开(公告)日：2023-07-04

申请号：US16633589

申请日：2018-07-25

Applicant: Spiber Inc. ,  Riken

Inventor： Keisuke Morita ,  Yunosuke Abe ,  Takehisa Maekawa ,  Koichi Kotaka ,  Junichi Sugahara ,  Keiji Numata

IPC: C07K14/435 ,  C12N15/63 ,  D01F4/02

CPC classification number: C07K14/43518 ,  C12N15/63 ,  D01F4/02

Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)nmotif-REP]m or Formula 2: [(A)nmotif-REP]m−(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin.

公开(公告)号：US20190177363A1

公开(公告)日：2019-06-13

申请号：US16324280

申请日：2017-08-10

Applicant: Spiber Inc.

Inventor： Toshiaki Osawa ,  Yuya Sato ,  Keisuke Morita

IPC: C07K1/34 ,  C07K1/30 ,  C12P21/02

Abstract: An object of the present invention is to provide a method for efficiently separating insoluble bodies of a recombinant protein from a recombinant cell expressing a target recombinant protein as insoluble bodies in the cell. The present invention provides a method for producing a recombinant protein aggregate by separating insoluble bodies of a recombinant protein from a recombinant cell expressing the recombinant protein as insoluble bodies in the cell, including disrupting the recombinant cell, aggregating the insoluble bodies of the recombinant protein, and separating the resulting aggregate.

公开(公告)号：US20190135880A1

公开(公告)日：2019-05-09

申请号：US16096818

申请日：2017-04-28

Applicant: Spiber Inc. ,  Kojima Industries Corporation

Inventor： Keisuke Morita ,  Hiroyuki Nakamura

IPC: C07K14/435 ,  C12N15/86 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  D01F4/02

Abstract: Provided is a modified fibroin, including a domain sequence represented by Formula 1: [(A)n motif-REP]m, in which the domain sequence has an amino acid sequence having a reduced content of (A)n motif equivalent to an amino acid sequence in which, at least, one or a plurality of the (A)n motifs is deleted, as compared to naturally occurring fibroin.[In Formula 1, (A)n motif represents an amino acid sequence consisting of 4 to 20 amino acid residues and the number of alanine residues relative to the total number of amino acid residues in the (A)n motif is 83% or more, REP represents an amino acid sequence consisting of 10 to 200 amino acid residues, m represents an integer of 8 to 300, and a plurality of (A)n motifs or the like may be the same amino acid sequence or different amino acid sequences.]

公开(公告)号：US09968682B2

公开(公告)日：2018-05-15

申请号：US14764455

申请日：2014-04-18

Applicant: SPIBER INC.

Inventor： Toshiaki Osawa ,  Keisuke Morita

IPC: A61K9/08 ,  A61K38/17 ,  A61L27/22 ,  A61L27/52 ,  A61K47/42 ,  A61L26/00 ,  A61L31/14 ,  A61K9/00 ,  C07K14/435 ,  A61L27/36 ,  A61L27/60

CPC classification number: A61K47/42 ,  A61K9/0087 ,  A61K38/1767 ,  A61L26/008 ,  A61L27/227 ,  A61L27/3604 ,  A61L27/3687 ,  A61L27/52 ,  A61L27/60 ,  A61L31/145 ,  A61L2430/06 ,  C07K14/43518

Abstract: A polypeptide hydrogel of the present invention is a hydrogel of a polypeptide derived from spider silk proteins, and has a moisture content of 85.0 to 99.9 mass % based on 100 mass % of the polypeptide hydrogel. A method for producing the hydrogel includes: a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide; and a substitution step in which the solution produced in the solution production step is substituted with a water-soluble solvent. Thus, the present invention provides a polypeptide hydrogel having excellent water retentivity and a polypeptide hydrogel suitable for application to a living body.