SUPER-RESOLUTION LATTICE LIGHT FIELD MICROSCOPIC IMAGING SYSTEM AND METHOD

    公开(公告)号:US20200242735A1

    公开(公告)日:2020-07-30

    申请号:US16357850

    申请日:2019-03-19

    Abstract: A super-resolution lattice light field microscopic imaging system includes: a microscope configured to magnify a sample and image the sample onto a first image plane of the microscope; a first relay lens configured to match a numerical aperture of an objective with that of a microlens array; a 2D scanning galvo configured to rotate an angle of a light path in the frequency domain plane; an illuminating system configured to provide uniform illumination on the microlens array to generate SIM pattern illumination; the microlens array, configured to modulate a light beam with a preset angle to a target spatial position at a back focal plane of the microlens array to obtain a modulated image; an image sensor configured to record the modulated image; and a reconstruction module configured to reconstruct a 3D structure of the sample based on the modulated image.

    MICROSCOPIC IMAGING SYSTEM AND METHOD WITH THREE-DIMENSIONAL REFRACTIVE INDEX TOMOGRAPHY

    公开(公告)号:US20190005701A1

    公开(公告)日:2019-01-03

    申请号:US15944403

    申请日:2018-04-03

    Abstract: Microscopic imaging system and method with three-dimensional refractive index tomography are provided. The microscopic imaging system includes: an illumination providing module, configured to provide a beam of parallel lights with a modulated intensity; a microscopic sample, arranged at downstream of the illumination providing module, and configured to modulate a phase of the beam of parallel lights, such that emergent lights passing through the microscopic sample carry information of a three-dimensional refractive index field of the microscopic sample; a microscopic imaging module, arranged at downstream of the microscopic sample, and configured to form an image by using the emergent lights; and a controlling module, configured to process the image to reconstruct three-dimensional refractive index information of the microscopic sample.

    Method for transmitting spectrum information
    14.
    发明授权
    Method for transmitting spectrum information 有权
    发送频谱信息的方法

    公开(公告)号:US08866972B1

    公开(公告)日:2014-10-21

    申请号:US14279848

    申请日:2014-05-16

    CPC classification number: G01J3/28 G01J2003/2826

    Abstract: A method for transmitting spectrum information is provided. The method includes: sampling a first video of a scene by a first sampling device and sampling a spectrum video of a sampling point in the scene by a second sampling device, and processing the spectrum video to obtain a spectrum information of the sampling point; calculating estimated transmission ratio coefficients of the spectrum video according to color integral curves of the first sampling device; estimating a location of the sampling point in each frame of the first video; and transmitting the spectrum information of the sampling point to a plurality of scene points in the first video according to the estimated transmission ratio coefficients and the location of the sampling point in each frame of the spectrum video through a trilateral filtering algorithm.

    Abstract translation: 提供了一种用于发送频谱信息的方法。 该方法包括:通过第一采样装置对场景的第一视频进行采样,并通过第二采样装置对场景中的采样点的频谱视频进行采样,并处理频谱以获得采样点的频谱信息; 根据第一采样装置的色彩积分曲线计算频谱视频的估计传输比系数; 估计第一视频的每个帧中的采样点的位置; 并且通过三边滤波算法,根据估计的传输比系数和频谱视频的每个帧中的采样点的位置,将采样点的频谱信息发送到第一视频中的多个场景点。

    High-resolution scanning light field microscopic imaging system and method

    公开(公告)号:US11131841B2

    公开(公告)日:2021-09-28

    申请号:US16357870

    申请日:2019-03-19

    Abstract: A scanning light field microscopic imaging system includes: a microscope configured to magnify a sample and image the sample onto a first image plane of the microscope; a relay lens configured to magnify or minify the first image plane; a 2D scanning galvo configured to rotate an angle of a light path in the frequency domain plane; the microlens array configured to modulate a beam with a preset angle to a target spatial position at a back focal plane of the microlens array and modulate the first image plane to obtain a modulated image; an image sensor configured to record the modulated image; and a reconstruction module configured to reconstruct a 3D structure of the sample based on the modulated image acquired from the image sensor.

    THREE-DIMENSIONAL MICROSCOPIC IMAGING METHOD AND SYSTEM

    公开(公告)号:US20200241276A1

    公开(公告)日:2020-07-30

    申请号:US16357811

    申请日:2019-03-19

    Abstract: Provided are a 3D microscopic imaging method and a 3D microscopic imaging system. The method includes: acquiring a first PSF of a 3D sample from an object plane to a plane of a main camera sensor and a second PSF of the 3D sample from the object plane to a plane of a secondary camera sensor, and generating a first forward projection matrix corresponding to the first PSF and a second forward projection matrix corresponding to the second PSF; acquiring a light field image captured by the main camera sensor and a high resolution image captured by the secondary camera sensor; generating a reconstruction result of the 3D sample by reconstructing the light field image, the first forward projection matrix, the high resolution image and the second forward projection matrix according to a preset algorithm.

    HIGH-RESOLUTION SCANNING LIGHT FIELD MICROSCOPIC IMAGING SYSTEM AND METHOD

    公开(公告)号:US20200241274A1

    公开(公告)日:2020-07-30

    申请号:US16357870

    申请日:2019-03-19

    Abstract: A scanning light field microscopic imaging system includes: a microscope configured to magnify a sample and image the sample onto a first image plane of the microscope; a relay lens configured to magnify or minify the first image plane; a 2D scanning galvo configured to rotate an angle of a light path in the frequency domain plane; the microlens array configured to modulate a beam with a preset angle to a target spatial position at a back focal plane of the microlens array and modulate the first image plane to obtain a modulated image; an image sensor configured to record the modulated image; and a reconstruction module configured to reconstruct a 3D structure of the sample based on the modulated image acquired from the image sensor.

    Structured illumination microscopy imaging system based on line-scanning spatiotemporal focusing

    公开(公告)号:US10539506B2

    公开(公告)日:2020-01-21

    申请号:US15790714

    申请日:2017-10-23

    Abstract: A microscopy imaging system is disclosed. The system includes: a femtosecond laser; an acousto-optic modulator, configured to periodically modulate an intensity of a laser light; a line-scanning component, configured to focus the laser light to form a line-shaped beam and to scan in a direction perpendicular to the line-shaped beam; a chromatic dispersion component, configured to generate spatial chirped laser pulses; a collimating lens, configured to converge components with different wavelengths dispersed by the chromatic dispersion component to propagate in parallel; a microscope component, configured to guide light passing through the collimating lens to illuminate the sample and capture a fluorescence image at a focal plane; and a synchronous control component, configured to synchronously control the acousto-optic modulator to modulate the intensity of the laser light, the line-scanning component to scan and the microscope component to capture fluorescence images, such that a reconstructed image is obtained according to the images.

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