公开(公告)号：US08496123B2

公开(公告)日：2013-07-30

申请号：US11995979

申请日：2006-07-24

Applicant: Andreas Axen ,  Anders Hagvall ,  Anders Larsson ,  Nicolas Thevenin

Inventor： Andreas Axen ,  Anders Hagvall ,  Anders Larsson ,  Nicolas Thevenin

IPC: B01D39/18 ,  B01D39/04 ,  B01D71/00

CPC classification number: B01D67/0093 ,  B01D71/14 ,  B01D2323/30 ,  B01J20/24 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/288 ,  B01J20/30 ,  B01J20/3219 ,  B01J20/3244 ,  B01J2220/4825 ,  C08B15/005 ,  C08J5/2287 ,  C08J2301/10

Abstract: The invention relates to a process for making porous cross-linked cellulose membranes and processes for coupling a chromatography ligand to cross-linked cellulose membranes. The invention provides methods for separating a first component from a second component in a solution based upon a difference in the size of the first and second components, and methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins from cell lysates and cultures.

Abstract translation: 本发明涉及制备多孔交联纤维素膜的方法和将色谱配体偶联到交联的纤维素膜上的方法。 本发明提供了基于第一和第二组分的尺寸差异将溶液中的第一组分与第二组分分离的方法，以及用于在溶液中从其它组分分离目标分子的方法，所述方法包括使用可通过该方法获得的膜 的本发明。 该方法在从细胞裂解物和培养物中分离蛋白质方面具有特殊的用途。

公开(公告)号：US20130074580A1

公开(公告)日：2013-03-28

申请号：US13625659

申请日：2012-09-24

Applicant: SIGMA-ALDRICH CO. LLC

Inventor： Daniel W. Armstrong

IPC: G01N1/22

CPC classification number: G01N30/62 ,  B01D53/02 ,  B01J20/288 ,  B01J20/29 ,  B01J20/3204 ,  B01J20/3253 ,  B01J20/3257 ,  B01J20/3282 ,  B01J20/3285 ,  B01J20/3287 ,  B01J2220/84 ,  B01J2220/86 ,  G01N1/22 ,  G01N2030/025 ,  G01N2030/8872

Abstract: This disclosure provides methods and devices for quantitating, separating and/or detecting water in a liquid, gas or solid sample comprising one or more chemicals, the method comprising: providing the liquid, gas or solid sample comprising water and the one or more chemicals; and exposing said liquid, gas or solid sample to at least one solid support including at least one dicationic and/or tricationic species of Formula I or II adsorbed, absorbed or immobilized on the solid support.

Abstract translation: 本公开提供了用于定量，分离和/或检测包含一种或多种化学品的液体，气体或固体样品中的水的方法和装置，所述方法包括：提供包含水和所述一种或多种化学品的液体，气体或固体样品; 以及将所述液体，气体或固体样品暴露于至少一种固体支持物，所述至少一种固体支持物包含吸收，吸收或固定在固体支持物上的式I或II的至少一种双阳离子和/或化合物种。

公开(公告)号：US20130041135A1

公开(公告)日：2013-02-14

申请号：US13636410

申请日：2011-03-23

Applicant: Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

Inventor： Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

IPC: C07K17/02 ,  C07K1/22

CPC classification number: C07K1/22 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/288 ,  B01J20/3219 ,  B01J20/3274 ,  C07K2317/10

Abstract: Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. R—R2   (1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R2.

Abstract translation: 提供了具有优异的耐碱性的亲和色谱法的填充剂和分离免疫球蛋白的方法。 用于亲和层析的填料是其中由下式（1）表示的蛋白质固定在载体上的填料。 R-R2（1）其中R表示由4至300个氨基酸残基组成的氨基酸序列，其含有由4至20个连续组氨酸残基组成的区域; 并且R 2表示能够结合免疫球蛋白的氨基酸序列，所述氨基酸序列由含有蛋白A的Z结构域或其片段的50至500个氨基酸残基或其变体组成，条件是R与C-末端结合 或R2的N-末端。

公开(公告)号：US20120205316A1

公开(公告)日：2012-08-16

申请号：US13398806

申请日：2012-02-16

Applicant: Taketoshi Kanda ,  Yasuo Igarashi ,  Kenichi Sakuma ,  Yousuke Toujo

Inventor： Taketoshi Kanda ,  Yasuo Igarashi ,  Kenichi Sakuma ,  Yousuke Toujo

IPC: B01D15/08

CPC classification number: B01D15/305 ,  B01D15/322 ,  B01D15/364 ,  B01J20/261 ,  B01J20/28016 ,  B01J20/28019 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3259 ,  B01J20/3285 ,  Y10T428/2982

Abstract: The object of the present invention is to provide a packing material and a separation method that manifest excellent hydrophilic interactions.The present invention provides a packing material for hydrophilic interaction chromatography consisting of a modified support treated with the surface modifier represented by the following formula (6) or (7).(In this formula, m denotes 2-6 and n denotes 1-4. X1, X2, and X3, independent of each other, denote a methoxy group, ethoxy group, or halogen. Up to two of X1, X2, and X3 can be any of the following groups: a methyl group, ethyl group, propyl group, isopropyl group, butyl group, or isobutyl group.)

Abstract translation: 本发明的目的是提供一种表现出优异的亲水相互作用的包装材料和分离方法。 本发明提供一种用于亲水相互作用色谱的包装材料，其由用下式（6）或（7）表示的表面改性剂处理的改性载体组成。 （在该式中，m表示2-6，n表示1-4，X1，X2，X3彼此独立地表示甲氧基，乙氧基或卤素，直至2个X 1，X 2，X 3 可以是甲基，乙基，丙基，异丙基，丁基或异丁基中的任一个。）

公开(公告)号：US08062526B2

公开(公告)日：2011-11-22

申请号：US12017662

申请日：2008-01-22

Applicant: Laurence Moine ,  David Colin Sherrington ,  Richard Alasdair Grant

Inventor： Laurence Moine ,  David Colin Sherrington ,  Richard Alasdair Grant

IPC: B01D15/08

CPC classification number: B01J20/288 ,  B01D15/265 ,  B01D15/322 ,  B01D15/424 ,  B01J20/261 ,  B01J20/262 ,  B01J20/264 ,  B01J20/267 ,  B01J20/28016 ,  B01J20/28023 ,  B01J20/28047 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3251 ,  B01J20/3255 ,  B01J2220/54 ,  B01J2220/58 ,  C22B3/42 ,  C22B11/04 ,  C22B11/046 ,  Y02P10/214 ,  Y02P10/234

Abstract: A process for the chromatographic interseparation of rhodium in admixture with at least one base metal from a feed solution includes complexes of these metals. The process includes contacting the solution with at least one chromatographic medium, and eluting one or more fractions such that at least one fraction includes rhodium complex substantially free from base metal contamination. The at least one chromatography medium includes a support functionalized with substituted amine groups which further include at least one hydrophilic group. Other platinum group metals can also be separated, both from one another and from base metals.

Abstract translation: 与来自进料溶液的至少一种贱金属混合的铑的色谱分离方法包括这些金属的复合物。 该方法包括使溶液与至少一种色谱介质接触，并洗脱一种或多种级分，使得至少一个级分包含基本上不含贱金属污染物的铑配合物。 至少一种色谱介质包括用取代的胺基官能化的载体，其还包括至少一个亲水基团。 其他铂族金属也可以彼此分离，从基体金属分离。

公开(公告)号：US07915318B2

公开(公告)日：2011-03-29

申请号：US12207055

申请日：2008-09-09

Applicant: Su Lu ,  Liangliang Qiang ,  Yanrong Zhu ,  Zhida Pan

Inventor： Su Lu ,  Liangliang Qiang ,  Yanrong Zhu ,  Zhida Pan

IPC: C08F2/44 ,  B01J20/26 ,  C08F4/80

CPC classification number: C08F290/00 ,  B01J20/28042 ,  B01J20/286 ,  B01J20/288 ,  C08F255/00 ,  C08F255/08 ,  C08F277/00 ,  C08F290/06 ,  C08F291/00 ,  C08G61/08 ,  C08L51/003 ,  C08L51/06 ,  G01N2030/528 ,  C08L2666/02

Abstract: A water soluble ROMP catalyst, which has high catalytic efficiency for ring opening metathesis is use in the preparation of polymeric blends for use in monolithic columns. In one embodiments a method for making a polymeric blend is discolosed comprising reaction of a water-soluble ROMP catalyst with a norbornene-substituted cycloolefin polymer or a polynorbornene polymer precursor, and wherein the contacting occurs in an aqueous or polar solvent in the presence of a solid support. In another embodiment a monolithic bed is disclosed comprising the water-soluble ROMP catalyst immobilized on a surface of a solid support. Also disclosed is a monolithic bed comprising: a polymer blend, the polymer blend produced from contacting a water soluble ROMP catalyst with a norbornene-substituted cycloolefin polymer or a polynorbornene polymer precursor; and wherein the contacting occurs in an aqueous or polar solvent in the presence of a solid support.

Abstract translation: 对于开环复分解具有高催化效率的水溶性ROMP催化剂用于制备用于整体柱的聚合物共混物。 在一个实施方案中，制造聚合物共混物的方法被解开，包括水溶性ROMP催化剂与降冰片烯取代的环烯烃聚合物或聚降冰片烯聚合物前体的反应，并且其中所述接触在水溶液或极性溶剂中，在 坚实的支持。 在另一个实施方案中，公开了一种整体床，其包含固定在固体载体表面上的水溶性ROMP催化剂。 还公开了一种整体床，包括：聚合物共混物，通过使水溶性ROMP催化剂与降冰片烯取代的环烯烃聚合物或聚降冰片烯聚合物前体接触而制备的聚合物共混物; 并且其中所述接触在固体载体存在下在水性或极性溶剂中进行。

公开(公告)号：US20090294362A1

公开(公告)日：2009-12-03

申请号：US12130493

申请日：2008-05-30

Applicant: Jonas Par PERSSON ,  Petrus Hans Hemstrom ,  Knur Irgum

Inventor： Jonas Par PERSSON ,  Petrus Hans Hemstrom ,  Knur Irgum

IPC: B01D15/08

CPC classification number: B01D15/325 ,  B01D15/305 ,  B01D15/322 ,  B01J20/286 ,  B01J20/288 ,  B01J20/3242 ,  B01J20/327 ,  B01J20/3278

Abstract: The present invention relates to the use of a stationary phase with neutral hydrophilic endgroups like sugar residues or polyole residues as sorbent for Hydrophilic interaction chromatography.

Abstract translation: 本发明涉及使用具有中性亲水端基（如糖残基或聚烯残基）的固定相作为亲水相互作用层析的吸附剂。

公开(公告)号：US20090278267A1

公开(公告)日：2009-11-12

申请号：US12506444

申请日：2009-07-21

Applicant: HSIN-CHIN HUNG

Inventor： HSIN-CHIN HUNG

IPC: G02B6/132

CPC classification number: B01J20/3278 ,  B01D15/32 ,  B01D15/362 ,  B01D15/38 ,  B01D15/424 ,  B01J20/286 ,  B01J20/288 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/328

Abstract: A method for manufacturing a light guide plate, includes: providing a first mold having a first contact surface, a second mold, and a reflector having a reflecting surface, the first mold defining a cavity from the first contact surface toward an inner portion thereof and having a gate communicated with the cavity; sandwiching the reflector between the first and second molds with the reflecting surface tightly contacting with the contact surface to enclose the cavity; injecting molten transparent resin into the cavity through the gate to fill the cavity up with the molten transparent resin, thereby forming a light guide plate body on the reflecting surface of the reflector; cooling the light guide plate body to solidify the light guide plate body in the cavity; and separating the first and second molds.

Abstract translation: 一种制造导光板的方法，包括：提供具有第一接触表面的第一模具，第二模具和具有反射表面的反射器，所述第一模具从所述第一接触表面朝向其内部限定空腔， 具有与腔连通的门; 将反射器夹在第一和第二模具之间，反射表面与接触表面紧密接触以封闭空腔; 通过浇口将熔融的透明树脂注入到空腔中，以用熔融的透明树脂填充空腔，从而在反射器的反射表面上形成导光板体; 冷却导光板体，使导光板体在腔内固化; 并分离第一和第二模具。

公开(公告)号：US07557232B2

公开(公告)日：2009-07-07

申请号：US11753934

申请日：2007-05-25

Applicant: Xiaodong Liu ,  Christopher A. Pohl ,  Jinhua Chen

Inventor： Xiaodong Liu ,  Christopher A. Pohl ,  Jinhua Chen

IPC: C07F7/02 ,  C07F7/08 ,  C07F7/18

CPC classification number: B01J20/288 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3257 ,  B01J20/3259 ,  B01J20/3263 ,  B01J20/3285

Abstract: The current invention provides compositions, which are useful as stationary phases for a variety of chromatographic applications, such as high performance liquid chromatography (HPLC). The compositions include a substrate (e.g., silica gel), covalently bound to a compound, which includes both a hydrophobic moiety and a hydrophilic moiety, which is preferably a 1,2-diol moiety. The hydrophobic moiety is sufficiently hydrophobic for the compositions to exhibit reversed phase characteristics and typically incorporates at least 5 carbon atoms in sequence. Based on having both hydrophilic and hydrophobic functionalities, the new stationary phases exhibit unique chromatographic properties. For example, these media can be used in either hydrophilic (HILIC) mode, in which the mobile phase includes a high percentage of an organic solvent, or in reversed phase mode, in which the mobile phase contains a higher percentage of an aqueous solvent. The current invention also provides methods of making and using the compounds and compositions of the invention.

Abstract translation: 本发明提供组合物，其可用作多种色谱应用的固定相，例如高效液相色谱（HPLC）。 组合物包括与包含疏水部分和亲水部分的化合物共价结合的底物（例如硅胶），其优选为1,2-二醇部分。 疏水部分足够疏水以使组合物显示反相特性，并通常依次包含至少5个碳原子。 基于具有亲水和疏水官能度，新的固定相显示出独特的色谱性质。 例如，这些介质可用于亲水（HILIC）模式，其中流动相包含高百分比的有机溶剂，或以相反模式使用，其中流动相含有较高百分比的水性溶剂。 本发明还提供了制备和使用本发明化合物和组合物的方法。

公开(公告)号：US07402243B2

公开(公告)日：2008-07-22

申请号：US10938172

申请日：2004-09-10

Applicant: Xiadong Liu ,  Christopher A. Pohl ,  Andrei V. Bordunov ,  Nebojsa Avdalovic

Inventor： Xiadong Liu ,  Christopher A. Pohl ,  Andrei V. Bordunov ,  Nebojsa Avdalovic

IPC: B01D15/08

CPC classification number: B01J20/288 ,  B01J20/286 ,  B01J20/287 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3285 ,  C07F7/1804

Abstract: The present invention provides novel silicon compounds, methods for making these novel silicon compounds, compositions comprising these novel silicon compounds attached to substrates, methods for attaching the novel silicon compounds to substrates and methods for using the compositions in a variety of chromatographic applications.

Abstract translation: 本发明提供新颖的硅化合物，制备这些新型硅化合物的方法，包含这些新型硅化合物附着在底物上的组合物，将新型硅化合物附着于底物的方法以及在各种色谱应用中使用该组合物的方法。