公开(公告)号：US11833526B2

公开(公告)日：2023-12-05

申请号：US17704820

申请日：2022-03-25

Applicant: Ancera Inc.

Inventor： Hur Koser

IPC: B03C1/253 ,  B03C1/023 ,  B03C1/32

CPC classification number: B03C1/253 ,  B03C1/023 ,  B03C1/32 ,  B01L2400/043 ,  B03C2201/18 ,  B03C2201/26

Abstract: Devices, methods, and systems are provided for extracting particles from a ferrofluid. Such methods may comprise receiving a flow of ferrofluid comprising target particles and background particles and generating a first, focusing magnetic field to focus the target particles towards a capture region. The capture region may capture the target particles and a plurality of background particles. A second, defocusing magnetic field may be configured to remove background particles from the capture region. A detector may be used to detect the target particles bound to the target region.

公开(公告)号：US20230384302A1

公开(公告)日：2023-11-30

申请号：US18139242

申请日：2023-04-25

Applicant: Inguran, LLC

Inventor： Kristie Krug

IPC: G01N33/543 ,  B03C1/015 ,  B03C1/02 ,  B03C1/023 ,  B03C1/01 ,  B03C1/28 ,  C12N13/00 ,  G01N33/569

CPC classification number: G01N33/5434 ,  B03C1/015 ,  B03C1/02 ,  G01N33/54346 ,  G01N33/54353 ,  B03C1/023 ,  B03C1/01 ,  B03C1/288 ,  G01N33/54333 ,  C12N13/00 ,  G01N33/56966 ,  B03C2201/18 ,  B03C2201/26

Abstract: A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.

公开(公告)号：US11774444B2

公开(公告)日：2023-10-03

申请号：US16349235

申请日：2017-11-10

Applicant: Beth Israel Deaconess Medical Center, Inc.

Inventor： Ionita C. Ghiran ,  Nathan Shapiro ,  Anish V. Sharda ,  Edward J. Felton

IPC: G01N33/543 ,  B03C1/01 ,  B03C1/033 ,  B03C1/30 ,  B03C1/32 ,  G01N9/36 ,  G01N33/50 ,  G01N35/00 ,  G01N33/569 ,  G01N33/537

CPC classification number: G01N33/54326 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/30 ,  B03C1/32 ,  G01N9/36 ,  G01N33/5005 ,  G01N33/5044 ,  G01N33/537 ,  G01N33/56966 ,  G01N33/56972 ,  G01N35/0098 ,  B03C2201/26

Abstract: Methods and apparatus for detecting cell-bound and soluble antigens in a biological sample are described. The method comprises forming complexes of at least one antibody-coated bead and at least one antigen in a solution, placing the solution in a magnetic field such that the formed complexes levitate in the solution at a particular height, and determining at least one characteristic of the antigen in the complexes based, at least in part, on an image of the complexes showing the magnetic levitation height.

公开(公告)号：US11772094B2

公开(公告)日：2023-10-03

申请号：US16827448

申请日：2020-03-23

Applicant: Daegu Gyeongbuk Institute of Science and Technology

Inventor： Min Seok Kim

IPC: C12N5/09 ,  C12N15/10 ,  B01L3/00 ,  B01D21/00 ,  B01D21/26 ,  B03C1/01 ,  B03C1/28 ,  B03C1/30

CPC classification number: B01L3/502753 ,  B01D21/0009 ,  B01D21/262 ,  B01L3/502738 ,  B03C1/01 ,  B03C1/288 ,  B03C1/30 ,  B01L2200/0647 ,  B01L2300/047 ,  B01L2400/06 ,  B03C2201/18 ,  B03C2201/26

Abstract: Provided are a microfluidic apparatus and a method for separating a target cell using the same. The microfluidic apparatus for separating a first material in a biological sample from the biological sample, according to an embodiment of the disclosure, includes: a body rotatable on a rotation axis; a mixing chamber included in the body, wherein the biological sample and magnetic beads that are combined with a second material in the biological sample are mixed in the mixing chamber; a separation chamber included in the body and connected to the mixing chamber, wherein a mixed sample of the first material and the second material combined with the magnetic beads is separated in the separation chamber; and a magnetic member positioned on one side of the body outside the separation chamber, wherein, in the separation chamber, the first material is separated from the second material combined with the magnetic beads by a centrifugal force and a magnetic force.

公开(公告)号：USRE49625E1

公开(公告)日：2023-08-29

申请号：US16845761

申请日：2020-04-10

Applicant: Arizona Board of Regents, a Body Corporate of the State of Arizona, Acting For and on Behalf of Arizona State University

Inventor： Michael Sierks ,  Mark Hayes

IPC: C07K14/47 ,  B03C5/00 ,  B03C5/02 ,  G01N27/447 ,  B01L3/00

CPC classification number: C07K14/4711 ,  B03C5/005 ,  B03C5/026 ,  G01N27/447 ,  B01L3/5027 ,  B03C2201/26

Abstract: The present invention provides devices and methods to separate and concentrate target protein species at a microliter scale and to generate reagents to those variants with exquisite selectivity for specific protein isoforms using only picograms of target material.

公开(公告)号：US11732293B2

公开(公告)日：2023-08-22

申请号：US16649894

申请日：2018-10-05

Applicant: The Board of Trustees of the University of Illinois

Inventor： Rashid Bashir ,  Anurup Ganguli

IPC: C12Q1/68 ,  C12Q1/6848 ,  B01L3/00 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/689 ,  G01N27/414 ,  B03C1/01 ,  B03C1/28 ,  B03C1/30

CPC classification number: C12Q1/6848 ,  B01L3/5027 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q1/6851 ,  G01N27/4145 ,  B01L2200/0689 ,  B01L2300/0861 ,  B01L2300/0893 ,  B01L2300/161 ,  B03C1/01 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26

Abstract: Described herein are systems and methods which utilize an array of wells to isolate pathogens and nucleic acid detection techniques to accurately and rapidly detect pathogens in fluid samples, even in very low concentrations, including from solid or semi-solid samples that have been fluidized. The provided systems and methods dry the fluid sample to deposit a fraction of the total volume in a number of wells and perform nucleic acid detection on individual wells to detect even individual pathogens and provide a quantitative analysis of the amount of pathogen within the sample. Also provided are methods and systems for precise delivery of dried materials, including biomolecules that are enzymes of use in the process, to microwells.

公开(公告)号：US11680258B2

公开(公告)日：2023-06-20

申请号：US17759417

申请日：2021-02-01

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Alex Hofai Lee ,  Daniel Chu

IPC: C12N15/10 ,  B01L3/00 ,  B01F31/441 ,  B01F31/44 ,  B01F33/452 ,  B03C1/28 ,  B03C1/01 ,  B01F33/81 ,  B01F101/23

CPC classification number: C12N15/1013 ,  B01F31/441 ,  B01F31/449 ,  B01F33/452 ,  B01F33/813 ,  B01L3/5085 ,  B03C1/01 ,  B03C1/288 ,  B01F2101/23 ,  B01L2200/0647 ,  B03C2201/18 ,  B03C2201/26

Abstract: An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.

公开(公告)号：US20230176062A1

公开(公告)日：2023-06-08

申请号：US18083279

申请日：2022-12-16

Applicant: Miltenyi Biotec B.V. & Co. KG

Inventor： Stefan Miltenyi ,  Winfried Schimmelpfennig ,  Holger Lantow ,  Niklas Elmar Neuschaefer ,  Martin Biehl ,  Eiad Kabaha ,  Juergen Schulz

IPC: G01N33/58 ,  A61M1/36 ,  B03C1/28 ,  B04B5/04 ,  B04B13/00 ,  B03C1/032 ,  B03C1/00 ,  B03C1/027 ,  B03C1/033 ,  B03C1/034 ,  A61M1/02 ,  G01N35/00 ,  C12N5/00 ,  C12M1/26 ,  C12M1/00 ,  B01D21/26 ,  B04B5/00 ,  B04B5/10 ,  B04B1/12 ,  B04B7/08 ,  B01D15/38 ,  C12N5/0789 ,  C12N5/077 ,  C12N5/0783 ,  C12M1/36

CPC classification number: G01N33/58 ,  A61M1/0209 ,  A61M1/3618 ,  A61M1/3633 ,  A61M1/3679 ,  A61M1/3692 ,  A61M1/3693 ,  A61M1/3696 ,  B01D15/3809 ,  B01D15/3885 ,  B01D21/26 ,  B03C1/002 ,  B03C1/027 ,  B03C1/032 ,  B03C1/034 ,  B03C1/288 ,  B03C1/0332 ,  B03C1/0335 ,  B04B1/12 ,  B04B5/00 ,  B04B5/04 ,  B04B5/10 ,  B04B5/0442 ,  B04B7/08 ,  B04B13/00 ,  C12M23/28 ,  C12M33/10 ,  C12M41/48 ,  C12M47/04 ,  C12N5/0018 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0647 ,  C12N5/0669 ,  G01N35/0098 ,  B01L3/5021 ,  B03C2201/18 ,  B03C2201/26

Abstract: The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder (42), a pump (64) and a plurality of valves (1-11) configured to at least partially control fluid flow through a fluid circuitry and a separation column (40) positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.

公开(公告)号：US20190240665A1

公开(公告)日：2019-08-08

申请号：US16160816

申请日：2018-10-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Matthew E. FOWLER ,  Phillip J. M. ELMS ,  Kevin D. LOUTHERBACK ,  Randall D. LOWE, JR. ,  Jian GONG ,  Tanner J. NEVILL ,  Gang F. WANG ,  Gregory G. LAVIEU ,  John A. TENNEY ,  Aathavan KARUNAKARAN

IPC: B01L3/00 ,  G06T7/00 ,  B01L9/00

CPC classification number: B01L3/502761 ,  B01L9/527 ,  B01L2300/0829 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2201/0675 ,  G06T7/0014

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20190134293A1

公开(公告)日：2019-05-09

申请号：US16099621

申请日：2017-05-09

Applicant: Jae-Hyung Robert CHANG ,  Giuseppe INTINI

Inventor： Jae-Hyung Robert CHANG ,  Giuseppe INTINI

IPC: A61M1/36 ,  A61M1/02 ,  B01D21/00

CPC classification number: A61M1/3618 ,  A61M1/0281 ,  A61M1/029 ,  A61M2202/0427 ,  B01D21/00 ,  B01D21/0009 ,  B03C1/01 ,  B03C2201/18 ,  B03C2201/26

Abstract: Provided is an accelerated method for preparing platelet rich plasma (PrP) without centrifugation or filtration. The method comprises contacting a sample of whole blood with an anti-coagulant and an inducer of Rouleaux formation; allowing the mixture to stand thereby depleting the sample of RBCs, and collecting the platelet-rich plasma fraction. The PrP volume obtained by the present method is about 10-60% of the volume of the starting whole blood sample, and contains less than 200,000 RBCs and at least 100,000 platelets per microliter.