公开(公告)号：US20170362579A1

公开(公告)日：2017-12-21

申请号：US15536279

申请日：2015-12-11

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Yi Feng Zheng ,  Yali Yang ,  Guoping Wang

IPC: C12N9/04 ,  G01N33/58 ,  G01N33/569 ,  G01N33/535 ,  C12Q1/28 ,  C07K16/44 ,  C07D405/12 ,  G01N33/94 ,  C07D317/58

CPC classification number: C12N9/0006 ,  C07D317/58 ,  C07D405/12 ,  C07D489/02 ,  C07D489/08 ,  C07K16/44 ,  C12Q1/28 ,  G01N33/535 ,  G01N33/56911 ,  G01N33/581 ,  G01N33/94 ,  G01N33/9486

Abstract: Compositions, methods, and kits are disclosed directed at haptens, immunogens and immunoassays for oxycodone and metabolites thereof. The compounds are exemplified by compounds of the Formula I. The method comprises providing in combination in a medium (i) a sample suspected of containing oxycodone and/or oxycodone metabolites, a compound of the Formula I wherein R4 or R5 is a label, and an antibody for oxycodone or a metabolite thereof. The medium is examined for the presence of a complex comprising the labeled compound of Formula I where the presence of such as complex indicates the presence of oxycodone or oxycodone metabolite in the sample.

公开(公告)号：US20170283845A1

公开(公告)日：2017-10-05

申请号：US15461081

申请日：2017-03-16

Applicant: Theranos, Inc.

Inventor： Elizabeth A. Holmes ,  Sunny Balwani

IPC: C12Q1/00 ,  G01N33/48 ,  C12Q1/68 ,  G01N30/02 ,  C12Q1/02 ,  C12Q1/28

CPC classification number: G01N35/02 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/28 ,  C12Q1/68 ,  G01N30/02 ,  G01N33/48 ,  G01N33/559 ,  G01N33/566 ,  G01N33/86 ,  G01N35/00 ,  G01N35/0092 ,  G01N2035/00326 ,  G01N2035/00495

Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

公开(公告)号：US20170260575A1

公开(公告)日：2017-09-14

申请号：US15456024

申请日：2017-03-10

Applicant: Emory University

Inventor： Khalid Salaita ,  Pui-yan Ma

IPC: C12Q1/68 ,  C12Q1/28

CPC classification number: C12Q1/6816 ,  C12Q1/28 ,  C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Y111/01007 ,  G01N33/542 ,  G01N2458/10 ,  C12Q2563/125 ,  C12Q2563/131

Abstract: This disclosure related to methods of detecting mechanical forces required to separate ligand and receptor interactions. In certain embodiments, this disclosure relates to methods of detecting mechanical forces between a ligand and receptor, where the ligand is immobilized on a surface using weaker forces. Ligand-receptor forces lead to dissociation of the ligand that can be detected and amplified. In certain embodiments, the disclosure relates to methods of detecting ligand and receptor interactions comprising linking a ligand to one of two binding partners, wherein the binding partners have attracting forces that are less than the forces between the ligand and a receptor of the ligand such that when the ligand binds the receptor, the binding partners will separate. Separation of the binding partners can be detected.

公开(公告)号：US20170212019A1

公开(公告)日：2017-07-27

申请号：US15483589

申请日：2017-04-10

Applicant: Ventana Medical Systems, Inc.

Inventor： Christopher Bieniarz ,  Brian D. Kelly ,  Jerome W. Kosmeder, II ,  Eric J. May ,  Larry Morrison

IPC: G01N1/30 ,  G01N33/58 ,  G01N33/543

CPC classification number: G01N1/30 ,  C12Q1/28 ,  G01N33/532 ,  G01N33/535 ,  G01N33/54393 ,  G01N33/581 ,  G01N2001/302

Abstract: Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

公开(公告)号：US09671400B2

公开(公告)日：2017-06-06

申请号：US12708702

申请日：2010-02-19

Applicant: Jesper Lohse

Inventor： Jesper Lohse

IPC: C12Q1/28 ,  G01N33/543 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48

CPC classification number: G01N33/54393 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48 ,  C12Q1/28

Abstract: The present invention relates to compounds which are conjugates of two or more moieties capable of serving as substrates of an enzyme with peroxidase activity and one or more labels. The conjugate molecules of the invention are useful for detection of molecular targets, e.g. biological or chemical molecules, molecular structures, etc, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc. The invention also relates to compositions comprising the conjugate molecules.

公开(公告)号：US20170114387A1

公开(公告)日：2017-04-27

申请号：US15301133

申请日：2015-03-17

Applicant: SHIGA UNIVERSITY OF MEDICAL SCIENCE

Inventor： Shin-ya MORITA

IPC: C12Q1/28 ,  G01N21/64 ,  C12Q1/32 ,  C12Q1/44 ,  C12Q1/48

CPC classification number: C12Q1/28 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/44 ,  C12Q1/485 ,  C12Y101/03021 ,  C12Y111/01007 ,  C12Y207/0103 ,  C12Y301/04004 ,  G01N21/6428 ,  G01N33/92 ,  G01N2333/902 ,  G01N2333/908 ,  G01N2333/91215 ,  G01N2333/916 ,  G01N2405/04

Abstract: The inventions disclosed herein are a method for quantifying cardiolipin in a sample, comprising the steps of: (1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand; and a kit for quantifying cardiolipin comprising phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase.

公开(公告)号：US09632102B2

公开(公告)日：2017-04-25

申请号：US13244949

申请日：2011-09-26

Applicant: Elizabeth Holmes ,  Sunny Balwani

Inventor： Elizabeth Holmes ,  Sunny Balwani

IPC: C12Q1/68 ,  G01N33/48 ,  G01N35/02 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/28 ,  G01N30/02 ,  G01N33/559 ,  G01N33/566 ,  G01N33/86 ,  G01N35/00

CPC classification number: G01N35/02 ,  C12Q1/00 ,  C12Q1/02 ,  C12Q1/28 ,  C12Q1/68 ,  G01N30/02 ,  G01N33/48 ,  G01N33/559 ,  G01N33/566 ,  G01N33/86 ,  G01N35/00 ,  G01N35/0092 ,  G01N2035/00326 ,  G01N2035/00495

Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

公开(公告)号：US09618429B2

公开(公告)日：2017-04-11

申请号：US13744504

申请日：2013-01-18

Applicant: Ventana Medical Systems, Inc.

Inventor： Eric J. May ,  Brian D. Kelly ,  Jerome W. Kosmeder ,  Larry Morrison ,  Christopher Bieniarz

IPC: G01N1/30 ,  G01N33/532 ,  C12Q1/28 ,  G01N33/535

CPC classification number: G01N1/30 ,  C12Q1/28 ,  G01N33/532 ,  G01N33/535 ,  G01N33/54393 ,  G01N33/581 ,  G01N2001/302

Abstract: Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

公开(公告)号：US09575065B2

公开(公告)日：2017-02-21

申请号：US15135730

申请日：2016-04-22

Applicant: THE CLEVELAND CLINIC FOUNDATION

Inventor： Stanley L. Hazen ,  Renliang Zhang

IPC: C12Q1/28 ,  G01N37/00 ,  G01N33/573 ,  G06F19/00 ,  G06Q50/22 ,  C12N1/28 ,  A61K31/616

CPC classification number: G01N33/573 ,  A61K31/616 ,  C12N1/28 ,  C12Q1/28 ,  C12Y111/01007 ,  G01N2333/908 ,  G01N2800/32 ,  G01N2800/324 ,  G01N2800/50 ,  G01N2800/52 ,  G06F19/00 ,  G06Q50/22 ,  G16H50/30 ,  Y02A90/22 ,  Y02A90/26

Abstract: Diagnostic tests for characterizing an individual's risk of developing or having a cardiovascular disease. In one embodiment the present diagnostic test comprises determining the level of myeloperoxidase (MPO) activity in a bodily sample obtained from the individual or test subject. In another embodiment, the diagnostic test comprises determining the level of MPO mass in a bodily sample obtained from the test subject. In another embodiment, the diagnostic test comprises determining the level of one or more select MPO-generated oxidation products in a bodily sample obtained from the test subject. The select MPO-generated oxidation products are dityrosine, nitrotyrosine, methionine sulphoxide or an MPO-generated lipid peroxidation products. Levels of MPO activity, MPO mass, or the select MPO-generated oxidation product in bodily samples from the test subject are then compared to a predetermined value that is derived from measurements of MPO activity, MPO mass, or the select MPO-generated oxidation product in comparable bodily samples obtained from the general population or a select population of human subjects. Such comparison characterizes the test subject's risk of developing CVD.

公开(公告)号：US09556471B2

公开(公告)日：2017-01-31

申请号：US11501296

申请日：2006-08-09

Applicant: Keith E. LeJuene ,  Jennifer A. Bacior ,  Jason A. Berberich ,  Markus Erbeldinger ,  Bhalchandra Shripad Lele

Inventor： Keith E. LeJuene ,  Jennifer A. Bacior ,  Jason A. Berberich ,  Markus Erbeldinger ,  Bhalchandra Shripad Lele

IPC: C12Q1/28 ,  C12M1/34 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/46

CPC classification number: C12Q1/28 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/46

Abstract: An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided.

Abstract translation: 一种含水酶的传感器，其包含一种或多种酶和至少一种能够指示至少一种酶与至少一种目标分析物的反应的指示剂化合物，其中酶和指示剂能够被递送，优选通过 喷射到具有目标分析物的表面，而不对表面进行取样。 提供了一种基于水性酶的传感器，其能够检测作为分析物的酶特异性底物。 该基于水性酶的传感器还包含一种或多种酶特异性底物，其中传感器检测酶抑制。 基于水性酶的传感器任选地包含一种或多种光散射添加剂，粘合剂聚合物，蛋白稳定剂，蛋白质稳定糖，表面活性剂和溶剂 （s）及其组合。 提供了一种分配系统和一种在不对表面进行取样的情况下在表面上检测目标化学物质的方法。