Abstract:
One aspect of the invention provides a liquid chromatography system including: a first solvent manager configured to dispense various ratios of a first solvent and a second solvent; a first column in fluid communication with the first solvent manager; a mixer in fluid communication with the first column; a first valve in fluid communication with the mixer; a second column having a first end in fluid communication with a first port of the first valve and a second end in fluid communication with a second port of the first valve; a second solvent manager adapted and configured to dispense various ratios of a third solvent and a fourth solvent; and a second valve in fluid communication with the second solvent manager, the first valve, and the mixer. The first valve and the second valve are adapted and configured for actuation between and a second position. In the first position: solvent dispensed by the first solvent manager and an injected sample flow over the first column; eluent from the first column is mixed with solvent dispensed by the second solvent manager in the mixer to produce a combined mobile phase; and the combined mobile phase is passed through the first valve and over the second column in a first direction to trap analytes of interest on the first column. In the second position, solvent dispensed by the second solvent manager is passed over the second column in a second direction to release the analytes of interest from the second column.
Abstract:
A liquid chromatography device includes an adsorption portion that adsorbs one or more analysis components in a specimen, an analysis device that analyzes an analysis component eluted by an eluent, a main feeding device that feeds a first eluent to the adsorption portion, the first eluent eluting an analysis component, a feeding channel in fluid communication with the main feeding device and the adsorption portion, a first retention channel that retains a second eluent, the second eluent differing from the first eluent, an auxiliary feeding device that feeds the second eluent to the first retention channel, and a first switching device that switches the feeding channel to either a first channel or a second channel, the first channel allowing the first eluent to flow from the main feeding device to the adsorption portion, and the second channel allowing the second eluent to flow from the first retention channel to the adsorption portion.
Abstract:
A sampler for liquid chromatography is described. The sampler includes an injection valve and a sample loop. The injection valve includes one waste port, two sample loop ports, and two high-pressure ports. The sample loop port includes a first loop part and a second loop part. The injection valve can be configured to have LOAD position and INJECT position. The injection valve can also be configured to have one or more additional positions such as a FULL PURGE position, a PUMP PURGE position, and a NEGATIVE PRESSURE position.
Abstract:
A fluid switching valve includes a first valve element (e.g., a stator) that has a plurality of first fluid-conveying features (e.g., ports), and a second valve element (e.g., a rotor) that has one or more second fluid-conveying features (e.g., fluid conduits in the form of grooves). The second valve element is movable, relative to the first valve element, between a plurality of discrete positions such that, in each of the discrete positions, at least one of the one or more second fluid-conveying features overlaps with multiple ones of the first fluid conveying features to provide for fluid communication therebetween. At least one of the first valve element and the second valve element includes a recess. The recess serves to reduce wear between the first valve element and the second valve element. The recess is arranged such that it does not overlap with any of the first fluid-conveying features or any of the second fluid-conveying features when the rotor is in any of the discrete positions.
Abstract:
Provided is a mechanism for connecting columns and needles, including: a base member 60 on which needles 61a and 61b with their tips directed upward are provided; and a column rack 10 having column holders for holding columns 10a and 10b in an upright position while allowing all upward shift of the columns, with an opening provided below each column holder. With the needles 61a and 61b positioned directly below the openings, the base member 60 is elevated until its upper surface comes in contact with the bottom of the column rack 10, whereby the needles 61a and 61b are inserted through the openings into the columns 20a and 20b. When the base member 60 is in contact with the column rack 10, the columns 20a and 20b are pushed by the needles 61a and 61b to a higher position within the column rack 10 than their original position.
Abstract:
A method of analysing gas chromatography data is described. The method, a first response factor data set acquired from a gas chromatograph (GC) apparatus during a procedure on a calibration or reference gas sample at a first time is received. One or more additional response factor data sets acquired from the gas chromatograph apparatus during a procedure on a calibration or reference gas sample from one or more later times are received. The method comprises calculating a measure of uncertainty for at least one compound of the reference gas sample from the first and additional response factor data sets. The one or more later times are during an operational period of the gas chromatograph apparatus. The measure of uncertainty may be used to, for example, identify the necessity to perform a maintenance action in the GC or to assess whether the GC is in a healthy or unhealthy condition.
Abstract:
A sample introduction device for reducing carry-over is provided. In the sample introduction device, when a high pressure valve 51 is switched from a load status to an injection status in a total volume injection method, the communication between a stator hole d in communication with an injection port 25 and a stator hole c connected to a flow path toward a separation/detection section 30 occurs earlier than the communication between a stator hole b in communication with a liquid feeder 10 and a stator hole a in communication with a needle 54. A length of a rotor groove Y communicating stator holes d and e or stator holes d and c is set longer than a length of another rotor groove X.
Abstract:
A channel-switching valve into which a high-pressure liquid and a low-pressure liquid flow is provided. The channel-switching valve includes a stator and a rotor which has a surface in contact with one surface of the stator and rotates while sliding on the contact surface. The stator has a plurality of liquid flow ports open to the contact surface, and the rotor has a plurality of channel grooves for connecting the liquid flow ports. One of the channel grooves is a straight groove passing through the rotational center of the rotor, and the other channel grooves are line-symmetrically arranged with respect to the straight channel groove. Alternatively, among the channel grooves, the channel grooves into which the high-pressure liquid flows are configured to be located on both sides of the rotational center of the rotor. Thus, a local load acting on the rotor during a high-pressure liquid-feeding process is reduced.
Abstract:
A channel switching valve is disclosed. The channel switching valve has a stator and a body section each having a connecting surface. The stator and the body section are removably fixed to each other by bolts at their connecting surfaces. Each of the connecting surfaces has a protruding/recessed pattern formed thereon, and the patterns are designed to fit into each other. By rotating the stator and the body section relative to each other, the stroke of a spring is changed between a position where the protruding/recessed patterns of the stator and the body section are fitted into each other and a position where they are not fitted into each other. The spring is held in the body section in its compressed state to urge the rotor toward the stator. An urging force for pressing the rotor against the stator is adjusted by changing the stroke of the spring.
Abstract:
Embodiments of the present invention are directed to methods and apparatus for placing a sample in a chromatographic system. The device and method feature placing samples held in a sample loop to pressurization prior to placing such sample loop in communication with high pressure conduits.