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    Multiview Light-Sheet Microscopy
    12.
    发明申请
    Multiview Light-Sheet Microscopy 审中-公开
    多视光照片显微镜

    公开(公告)号：US20160161410A1

    公开(公告)日：2016-06-09

    申请号：US15040545

    申请日：2016-02-10

    Applicant: Howard Hughes Medical Institute

    Inventor： Philipp Johannes Keller

    IPC: G01N21/64 ,  G02B21/16 ,  G02B27/10 ,  G02B21/00 ,  G02B21/36 ,  G01N33/483 ,  G02B21/06

    CPC classification number: G01N21/6486 ,  A61B5/0071 ,  A61B2503/42 ,  G01N21/64 ,  G01N33/4833 ,  G01N2021/1736 ,  G01N2201/06193 ,  G01N2201/064 ,  G02B21/0004 ,  G02B21/0048 ,  G02B21/06 ,  G02B21/16 ,  G02B21/361 ,  G02B21/362 ,  G02B21/367 ,  G02B27/1013

    Abstract: A live biological specimen is imaged by generating a plurality of light sheets; directing the plurality of light sheets along an illumination axis through the biological specimen such that the light sheets spatially and temporally overlap within the biological specimen along an image plane, and optically interact with the biological specimen within the image plane; and recording, at each of a plurality of views, images of the fluorescence emitted along a detection axis from the biological specimen due to the optical interaction between the light sheets and the biological specimen. The temporal overlap is within a time shift that is less than a resolution time that corresponds to a spatial resolution limit of the microscope.

    Abstract translation: 通过生成多个光片对活体生物样本进行成像; 沿着照明轴线引导多个光片通过生物样本，使得光片沿着图像平面在空间和时间上在生物样本内重叠，并与图像平面内的生物样本光学相互作用; 并且在多个视图的每一个处，由于光片和生物样本之间的光学相互作用，沿着检测轴从生物样本记录荧光的图像。 时间重叠在时间偏移之内，其小于对应于显微镜的空间分辨率极限的分辨率时间。

    Method to increase the number of detectable photons during the imaging of a biological marker
    13.
    发明授权
    Method to increase the number of detectable photons during the imaging of a biological marker 有权
    在生物标记成像过程中增加可检测光子数量的方法

    公开(公告)号：US09329132B2

    公开(公告)日：2016-05-03

    申请号：US13637551

    申请日：2011-03-25

    Applicant: Kelly Rogers ,  Spencer Shorte ,  Joseph Dragavon ,  Samantha Blazquez

    Inventor： Kelly Rogers ,  Spencer Shorte ,  Joseph Dragavon ,  Samantha Blazquez

    IPC: G01N21/64 ,  B82Y15/00

    CPC classification number: G01N21/6486 ,  B82Y15/00 ,  G01N21/6428 ,  G01N2201/06193

    Abstract: The present invention relates a method to determine the presence of a photon producing biological marker in a cell, tissue or organism of interest. The method is based on Fluorescence by Unbound Excitation from Luminescence (FUEL) and comprises the steps of a) providing conditions suitable for the biological marker to produce at least one first photon by luminescence; b) providing a FUEL probe pair-upper (FPP-U) disposed in proximity to the cell, tissue or organism, wherein the at least one first photon of step a) excites the FPP-U, which emits at least one second photon. The FPP-U may be selected from the group of quantum dots, carbon nanotubes, fluorescent proteins, diamond nanocrystals and metalloporphyrins. This method is characterized in that said biological marker and said FPP-U are not bound and in that each of the at least one second photon(s) are of a longer wavelength than each of the at least one first photon(s).

    Abstract translation: 本发明涉及确定感兴趣的细胞，组织或生物体中产生光子的生物标志物的存在的方法。 该方法基于通过未发光发光（FUEL）的荧光，并且包括以下步骤：a）提供适于生物标记物的条件以通过发光产生至少一个第一光子; b）提供设置在细胞，组织或生物体附近的FUEL探针对上（FPP-U），其中步骤a）的至少一个第一光子激发FPP-U，其发射至少一个第二光子。 FPP-U可以选自量子点，碳纳米管，荧光蛋白，金刚石纳米晶体和金属卟啉。 该方法的特征在于所述生物学标记和所述FPP-U不是结合的，并且所述至少一个第二光子中的每一个具有比所述至少一个第一光子中的每一个更长的波长。

    PATHOGEN DETECTION BY SIMULTANEOUS SIZE/FLUORESCENCE MEASUREMENT
    14.
    发明申请
    PATHOGEN DETECTION BY SIMULTANEOUS SIZE/FLUORESCENCE MEASUREMENT 审中-公开
    病原体同时检测大小/荧光测量

    公开(公告)号：US20150168288A1

    公开(公告)日：2015-06-18

    申请号：US14607655

    申请日：2015-01-28

    Applicant: Azbil Corporation

    Inventor： Erik H. BINNIE ,  Gregory Scott Morris

    IPC: G01N15/14 ,  G01N21/64 ,  G01N21/53

    CPC classification number: G01N15/1434 ,  G01N1/2247 ,  G01N1/2273 ,  G01N15/1404 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/6486 ,  G01N2015/0088 ,  G01N2015/025 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2201/06113 ,  G01N2201/06193

    Abstract: A method and apparatus for detecting pathogens and particles in a fluid in which particle size and intrinsic fluorescence of a simple particle is determined, comprising a sample cell; a light source on one side of the sample cell for sending a focused beam of light through the sample, whereby portions of the beam of light are scattered at various angles by particles of various sizes present in the sample area; a particle size detector positioned in the light path for detecting a portion of forward scattered light; a pair of fluorescence detectors positioned off axis from the beam of light; and a pair of elliptical mirrors positioned such that an intersection of the incoming particle stream and the light beam are at one foci of each ellipsoid, and one of said pair of fluorescence detectors lies at the other foci.

    Abstract translation: 一种用于检测流体中病原体和颗粒的方法和装置，其中确定了简单颗粒的粒度和固有荧光，包括样品池; 在样品池的一侧上的光源，用于发射聚焦光束穿过样品，由此部分光束以各种尺寸的颗粒以样品区域中存在的各种尺寸散射; 位于光路中的用于检测前向散射光的一部分的粒度检测器; 位于离光束离轴的一对荧光检测器; 以及一对椭圆镜，其定位成使得进入的粒子流和光束的交点处于每个椭圆体的一个焦点处，并且所述一对荧光检测器中的一个位于另一个焦点。

    BIOANALYTICAL INSTRUMENTATION USING A LIGHT SOURCE SUBSYSTEM
    17.
    发明申请
    BIOANALYTICAL INSTRUMENTATION USING A LIGHT SOURCE SUBSYSTEM 有权
    使用光源子系统的生物仪器

    公开(公告)号：US20120238472A1

    公开(公告)日：2012-09-20

    申请号：US13486404

    申请日：2012-06-01

    Applicant: Claudia B. Jaffe ,  Steven M. Jaffe ,  Michieal L. Jones

    Inventor： Claudia B. Jaffe ,  Steven M. Jaffe ,  Michieal L. Jones

    IPC: G01N21/64 ,  C40B30/10 ,  C40B60/12

    CPC classification number: G01J3/10 ,  G01N21/253 ,  G01N21/255 ,  G01N21/4788 ,  G01N21/51 ,  G01N21/53 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6469 ,  G01N2021/6484 ,  G01N2201/06193

    Abstract: The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems. The capillaries are used to separate molecules that are chemically tagged with appropriate fluorescent or chromophore groups.

    Abstract translation: 本发明涉及用于用存在于检测体积中的分子照射一种或多种所选波长的光并将荧光，吸光度，透射率，散射引导到一个或多个检测器上的光源。 与光的分子相互作用允许使用物理或化学标签（最常见的荧光和发色团标记）在反应中鉴定和定量参与的化学部分。 本发明还可以使用光源单独地并且同时用一个或多个选择的波长的光照射多个毛细管或其它流动约束结构，并且分别并且同时检测在毛细管或其它流动限制结构内产生的荧光。 在各种实施方案中，流动限制结构可以允许分子的分离或运输，并且包括毛细管，微孔和毫里流系统。 毛细血管用于分离用适当荧光或发色团组化学标记的分子。

    Optical oxygen concentration measurement method and optical oxygen concentration measuring sensor
    18.
    发明申请
    Optical oxygen concentration measurement method and optical oxygen concentration measuring sensor 审中-公开
    光学氧浓度测量方法和光学氧浓度测量传感器

    公开(公告)号：US20030235513A1

    公开(公告)日：2003-12-25

    申请号：US10459468

    申请日：2003-06-12

    Applicant: NATIONAL AEROSPACE LABORATORY OF JAPAN

    Inventor： Keisuke Asai ,  Hiroyuki Nishide ,  Ichiro Okura

    IPC: G01N021/00

    CPC classification number: G01N21/783 ,  G01N21/6428 ,  G01N2021/6432 ,  G01N2021/7786 ,  G01N2201/06193

    Abstract: The present invention provides an optical oxygen concentration measurement method and optical oxygen concentration measuring sensor wherein a light-absorbing dye-molecule layer whose absorption spectrum varies depending on the bonding with oxygen molecules is combined with a light-emitting layer, and the oxygen concentration of the ambient fluid can be measured. The light-absorbing layer 4 laminated to an oxygen-quenching light-emitting layer 3 is a film comprising a cobalt-porphyrin complex (CoP) or other light-absorbing dye molecule 7 whose absorption spectrum varies depending on the bonding with oxygen molecules. When the degree of overlap with the light emission spectrum or excitation (absorption) spectrum of the light-emitting layer 3 varies as a result of a variation in the absorption spectrum possessed by the light-absorbing layer 4, the light intensity of output light varies in accordance with the degree of overlap, and the oxygen concentration of the ambient fluid can be measured. These measurement method and measuring sensor can be applied not only to optical fiber sensors, but also to wind tunnel experiments and the like as pressure-sensitive paints.

    Abstract translation: 本发明提供一种光学氧浓度测定方法和光学氧浓度测定传感器，其中吸收光谱根据与氧分子的键合而变化的吸光染料分子层与发光层结合，氧浓度 可以测量环境流体。 层叠到氧熄光发光层3的光吸收层4是包含钴 - 卟啉络合物（CoP）或其吸收光谱根据与氧分子的键合而变化的其他吸光染料分子7的膜。 当发光层3的发光光谱或激发（吸收）光谱的重叠程度随着光吸收层4所具有的吸收光谱的变化而变化时，输出光的光强度变化 根据重叠程度，可以测量环境流体的氧浓度。 这些测量方法和测量传感器不仅可以应用于光纤传感器，还可以应用于风洞实验等作为压敏涂料。

    Method and devices for laser induced fluorescence attenuation spectroscopy
    19.
    发明授权
    Method and devices for laser induced fluorescence attenuation spectroscopy 失效
    激光诱导荧光衰减光谱法的方法和装置

    公开(公告)号：US6124597A

    公开(公告)日：2000-09-26

    申请号：US889017

    申请日：1997-07-07

    Applicant: Ramez E. N. Shehada ,  Vasilis Z. Marmarelis ,  Warren S. Grundfest

    Inventor： Ramez E. N. Shehada ,  Vasilis Z. Marmarelis ,  Warren S. Grundfest

    IPC: A61B5/00 ,  A61B5/145 ,  A61B5/1455 ,  A61B10/00 ,  G01N21/27 ,  G01N21/49 ,  G01N21/64

    CPC classification number: G01N21/6486 ,  A61B5/0075 ,  A61B5/0084 ,  G01N21/49 ,  G01N21/6402 ,  G01N21/6428 ,  A61B5/0071 ,  A61B5/0086 ,  A61B5/1455 ,  A61B5/7267 ,  G01N2021/6484 ,  G01N2201/06193

    Abstract: The Laser Induced Fluorescence Attenuation Spectroscopy (LIFAS) method and apparatus preferably include a source adapted to emit radiation that is directed at a sample volume in a sample to produce return light from the sample, such return light including modulated return light resulting from modulation by the sample, a first sensor, displaced by a first distance from the sample volume for monitoring the return light and generating a first signal indicative of the intensity of return light, a second sensor, displaced by a second distance from the sample volume, for monitoring the return light and generating a second signal indicative of the intensity of return light, and a processor associated with the first sensor and the second sensor and adapted to process the first and second signals so as to determine the modulation of the sample. The methods and devices of the inventions are particularly well-suited for determining the wavelength-dependent attenuation of a sample and using the attenuation to restore the intrinsic laser induced fluorescence of the sample. In turn, the attenuation and intrinsic laser induced fluorescence can be used to determined a characteristic of interest, such as the ischemic or hypoxic condition of biological tissue.

    Abstract translation: 激光诱导荧光衰减光谱法（LIFAS）方法和装置优选地包括适于发射针对样品中的样品体积的辐射以产生来自样品的返回光的源，这种返回光包括由调制的调制的返回光 样品，第一传感器，离开样品体积第一距离，用于监测返回光并产生指示返回光强度的第一信号;第二传感器，其从样品体积中移出第二距离，用于监测 返回光并产生指示返回光强度的第二信号，以及与第一传感器和第二传感器相关联并适于处理第一和第二信号以便确定样本的调制的处理器。 本发明的方法和装置特别适用于确定样品的波长相关衰减，并使用衰减来恢复样品的固有激光诱导荧光。 反过来，衰减和固有的激光诱导荧光可用于确定感兴趣的特征，例如生物组织的缺血或缺氧状况。

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