公开(公告)号：US20250129358A1

公开(公告)日：2025-04-24

申请号：US18923104

申请日：2024-10-22

Applicant: Promega Corporation

Inventor： Zhiyang Zeng ,  Andrew Taft ,  Kasen Riemersma ,  Spencer Hermanson ,  Wenhui Zhou

IPC: C12N15/10 ,  C08F26/06

Abstract: Provided herein are polymeric compounds, compositions, and methods for isolation of nucleic acids from a sample.

公开(公告)号：US20250102463A1

公开(公告)日：2025-03-27

申请号：US18750515

申请日：2024-06-21

Applicant: PROMEGA CORPORATION

Inventor： Sergey Lazarev ,  Bruce Johnson

IPC: G01N27/447 ,  G01J1/42 ,  G02B21/00 ,  G02B26/10 ,  G02B27/09

Abstract: A laser-line-generating system generates and process a laser light to illuminate a capillary array. The laser-line-generating system includes a laser-light source, focusing optics, a first optical scanner, and a second optical scanner. The laser-light source outputs a first laser light. The focusing optics receives the first laser light and reduces a beam width of the first laser light. The first optical scanner receives the first laser light and output a first optical-scanner-outputted light by varying an angle of outputted light along a first dimension. The second optical scanner receives the first optical-scanner-outputted light and outputs a second optical-scanner-outputted light by varying an angle of outputted light along a second dimension. The second optical-scanner-outputted light includes a line with dimensional components in both the first dimension and the second dimension.

公开(公告)号：US12221648B2

公开(公告)日：2025-02-11

申请号：US17374460

申请日：2021-07-13

Applicant: Promega Corporation

Inventor： Carolyn W. Hitko ,  Thomas Kirkland ,  Sergiy Levin ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Harry Tetsuo Uyeda ,  Ji Zhu

IPC: C12Q1/34 ,  C12N9/14 ,  G01N33/543

Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.

公开(公告)号：US12215375B2

公开(公告)日：2025-02-04

申请号：US17332459

申请日：2021-05-27

Applicant: Promega Corporation

Inventor： Brock Binkowski ,  Lance P. Encell ,  Mary Hall ,  Matthew B. Robers ,  Michael R. Slater ,  Keith V. Wood ,  Monika G. Wood

IPC: C12N9/02 ,  C07D487/04 ,  C12N15/52 ,  C12Q1/66

Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.

公开(公告)号：US12065421B2

公开(公告)日：2024-08-20

申请号：US17841613

申请日：2022-06-15

Applicant: PROMEGA CORPORATION

Inventor： Joel R. Walker ,  Mary Hall ,  Christopher Todd Eggers ,  Horacio Lazaro

IPC: C07D333/38 ,  C07C311/21 ,  C07C311/29 ,  C07C317/38 ,  C07D333/66 ,  C07D333/78 ,  G01N21/76 ,  G01N33/543 ,  G01N33/573

CPC classification number: C07D333/38 ,  C07C311/21 ,  C07C311/29 ,  C07C317/38 ,  C07D333/66 ,  C07D333/78 ,  G01N21/763 ,  G01N33/54306 ,  G01N33/573 ,  C07C2601/14 ,  C07C2602/10

Abstract: Compounds that may selectively inhibit Oplophorus luciferase-derived bioluminescent complexes, e.g., NanoBiT® bioluminescent complex, are disclosed as well as compositions and kits comprising the compounds, and methods of using the compounds.

公开(公告)号：US20240191211A1

公开(公告)日：2024-06-13

申请号：US18312487

申请日：2023-05-04

Applicant: Promega Corporation

Inventor： Rachel Friedman Ohana ,  Ce Shi ,  Robin Hurst ,  Mark A. Klein ,  Jian Cao ,  Thomas Kirkland ,  Connor Fitzgerald ,  Tetsuo Uyeda ,  Thomas Machleidt ,  Wenhui Zhou ,  Matthew A. Larsen ,  Hui Wang ,  Weiwei An ,  Karilyn Porter

IPC: C12N9/02 ,  B01J31/22 ,  B01J35/39

CPC classification number: C12N9/0069 ,  B01J31/2295 ,  B01J35/39 ,  C12Y113/12013

Abstract: Provided herein are systems, methods, and compositions for bioluminescence-triggered catalysis of bioorthogonal labeling chemistries in a proximity dependent manner. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts or photosensitizers, activatable labels, and systems thereof, and methods of catalytically activating the activatable labels via bioluminescence-triggered catalysis.

公开(公告)号：US20240060059A1

公开(公告)日：2024-02-22

申请号：US18311977

申请日：2023-05-04

Applicant: Promega Corporation

Inventor： Michael P. Killoran ,  Evan Glasgow ,  Lance P. Encell ,  Thomas Kirkland ,  Thomas Machleidt

IPC: C12N9/14 ,  C12N15/63

CPC classification number: C12N9/14 ,  C12N15/63 ,  C12Y308/01005

Abstract: Provided herein are circularly-permuted (cp) dehalogenase variants that are capable of covalently binding to a haloalkyl ligand. In particular cp dehalogenase variants and peptides and polypeptides comprising split versions thereof that structurally assemble to form an active dehalogenase complexes are provided.

公开(公告)号：US11781168B2

公开(公告)日：2023-10-10

申请号：US17240435

申请日：2021-04-26

Applicant: PROMEGA CORPORATION

Inventor： Anton Shakhmin ,  Thomas Kirkland ,  Joel Walker ,  Thomas Machleidt ,  Mary Hall ,  Keith V. Wood

IPC: C07D487/04 ,  C12Q1/66 ,  C07D519/00 ,  A61K49/00 ,  G01N33/58 ,  H04N1/60

CPC classification number: C12Q1/66 ,  A61K49/0013 ,  C07D487/04 ,  C07D519/00 ,  G01N33/581 ,  H04N1/6041 ,  G01N2333/90241

Abstract: Described are processes for preparing substituted imidazo[1,2-a]pyrazine compounds of formula (I):




by reacting a compound of formula (xi):





with carbonyldiimidazole to form the compound of formula (I).

公开(公告)号：US11692983B2

公开(公告)日：2023-07-04

申请号：US17159923

申请日：2021-01-27

Applicant: Promega Corporation

Inventor： Joshua Jacques Coon ,  Michael M. Rosenblatt ,  Marjeta Urh

IPC: G01N30/86 ,  G01N30/02 ,  H01J49/00 ,  G01N30/04 ,  G01N30/88

CPC classification number: G01N30/8665 ,  G01N2030/027 ,  G01N2030/047 ,  G01N2030/8831 ,  H01J49/0009

Abstract: The present invention provides reagents for instrumentation quality control and methods of use thereof. In particular, sets of peptides or other molecules are provided for evaluating the performance of instruments with mass spectrometry (MS) and/or liquid chromatography (LC) functionalities.

公开(公告)号：US20230194515A1

公开(公告)日：2023-06-22

申请号：US17819784

申请日：2022-08-15

Applicant: Promega Corporation

Inventor： Cesear Corona ,  Poncho Meisenheimer ,  Matthew Robers ,  James Vasta

IPC: G01N33/543 ,  C07D239/48 ,  G01N1/34 ,  G01N33/573

CPC classification number: G01N33/54353 ,  C07D239/48 ,  G01N1/34 ,  G01N33/573 ,  G01N21/6428

Abstract: Provided herein are compounds that are broad-spectrum protein kinase binding agents, detectable tracers comprising such compounds, and method of use thereof for the detection of protein kinases.