KIT FOR DETECTING MELANOMA
    27.
    发明申请

    公开(公告)号:US20220098673A1

    公开(公告)日:2022-03-31

    申请号:US17425611

    申请日:2020-01-23

    Abstract: The current invention relates to a kit for detecting melanoma. More particularly, it relates to an isolated polypeptide vGAF as a biomarker for detection of melanoma, which is also a DNA repair protein, and can be used for treatment of melanoma, specifically skin cutaneous melanoma. The invention encompasses novel methods and compositions for detection, prevention and treatment or melanoma, specifically skin cutaneous melanoma using vGAF.

    Modified chitinase from Brevibacillus laterosporus LAK 1210 and uses thereof

    公开(公告)号:US11168314B2

    公开(公告)日:2021-11-09

    申请号:US16122612

    申请日:2018-09-05

    Abstract: The present invention discloses a recombinant, modified extracellular chitinase having an amino acid sequence set forth in SEQ ID NO.4 or SEQ ID NO.5. The recombinant, modified chitinase is derived by inserting two-point mutations at positions 661 and 2158 in the native chitinase gene of Brevibacillus laterosporus LAK 1210 which results in amino acid substitution of tyrosine (Y) residue with histidine (H) at positions 221 and 720. The modified chitinase exhibits both exochitinase and endochitinase activity. The recombinant, modified enzyme is thermoactive with a temperature optimum of 55-60° C. and high thermostability (Tm of 66.7° C.), functions in a broad pH range (pH 3.0-11.0) having a pH optimum of 9.0 and also exhibits improved solubility and enhanced efficacy for control of insects and phytopathogenic fungi. The invention further provides improved methods for large scale production of recombinant modified chitinase and rapid, cost-effective purification method by chitin-adsorption affinity chromatography using powdered crustacean shells.

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