公开(公告)号：US09135700B2

公开(公告)日：2015-09-15

申请号：US13891301

申请日：2013-05-10

Applicant: Dako Denmark A/S

Inventor： Rohit Jain ,  Joachim Helmut Schmid ,  Mark Verardo ,  Jeffery Caron

IPC: G06K9/00 ,  G06T7/00 ,  G06T7/60

CPC classification number: G06T7/0012 ,  G06K9/0014 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T7/60 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30068

Abstract: Methods and systems for image scoring and analysis are provided. Scored and analyzed images may include digital pathology images. Image scoring and analysis methods may include techniques to identify nuclei and determine membrane staining extent through the use of a priori models. Image scoring and analysis methods may include techniques for membrane intensity determination. Images may be scored based on an extent of membrane staining and membrane intensity.

Abstract translation: 提供了图像评分和分析的方法和系统。 得分和分析的图像可以包括数字病理图像。 图像评分和分析方法可以包括通过使用先验模型识别细胞核并确定膜染色程度的技术。 图像评分和分析方法可以包括用于膜强度测定的技术。 可以根据膜染色和膜强度的程度对图像进行评分。

公开(公告)号：US09133507B2

公开(公告)日：2015-09-15

申请号：US14105346

申请日：2013-12-13

Applicant: DAKO DENMARK A/S

Inventor： Gregory A. Testa ,  Tim Svenstrup Poulsen ,  Steen Hauge Matthiesen ,  Ole Feldballe Rasmussen ,  Lars Winther

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00 ,  G01N1/31 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12Q1/6841 ,  B01L3/508 ,  B01L7/52 ,  B01L2200/142 ,  B01L2300/043 ,  B01L2300/069 ,  B01L2300/0822 ,  B01L2300/10 ,  B01L2300/1822 ,  B01L2300/1844 ,  C12M41/14 ,  C12M41/36 ,  G01N1/312 ,  Y10T436/143333

Abstract: An apparatus for processing at least one biological sample accommodated on at least one carrier member (15) in a chamber includes, at least one reservoir (18) able to accommodate a fluid on a surface inside the chamber adjacent to and/or facing a substantial part of the at least one biological sample. The apparatus may comprise a bottom member (12) arranged to support at least one carrier member (15) carrying at least one biological sample and a lid (14) including at least one fluid reservoir (18). The reservoir filled with water provides humidity to the chamber and impedes drying out of the sample.

Abstract translation: 用于处理容纳在腔室中的至少一个载体构件（15）上的至少一个生物样品的装置包括：至少一个储存器（18），其能够容纳与所述腔室内的表面相邻和/或面对实质的流体 至少一种生物样品的一部分。 该装置可以包括底部构件（12），该底部构件（12）被布置成支撑承载至少一个生物样品的至少一个载体构件（15）和包括至少一个流体储存器（18）的盖子（14）。 充满水的储存器为室提供湿气并阻止样品干燥。

公开(公告)号：US08999639B2

公开(公告)日：2015-04-07

申请号：US13856569

申请日：2013-04-04

Applicant: DAKO Denmark A/S

Inventor： Jesper Lohse

IPC: C12Q1/68 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48 ,  C12Q1/28 ,  G01N33/543 ,  C07H21/02 ,  C07H19/04

CPC classification number: G01N33/54393 ,  C08G63/672 ,  C08G69/40 ,  C08G69/48 ,  C12Q1/28

Abstract: The present invention relates to methods and compounds for detection of molecular targets, such as biological or chemical molecules, or molecular structures, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc.

Abstract translation: 本发明涉及用于检测分子靶标的方法和化合物，例如生物或化学分子或分子结构，使用大量用于检测和可视化这些靶标的实验方案的样品。 免疫组化（IHC），原位杂交（ISH），ELISA，Southern，Northern和Western blotting等。

公开(公告)号：US20150017647A1

公开(公告)日：2015-01-15

申请号：US14503925

申请日：2014-10-01

Applicant: DAKO DENMARK A/S

Inventor： Tim Svenstrup POULSEN ,  Steen Hauge MATTHIESEN

IPC: G01N1/30

CPC classification number: G01N1/2813 ,  G01N1/30 ,  G01N1/312

Abstract: An apparatus for processing a biological sample is provided. The biological sample being arranged on a first planar surface of a carrier, the apparatus having a second planar surface arranged substantially parallel to said first planar surface and at a first distance from said first planar surface. The first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP). The apparatus having a supply for supplying an amount of a liquid that is to be applied to said biological sample. The first planar surface and said second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

Abstract translation: 提供了一种用于处理生物样品的装置。 生物样品被布置在载体的第一平坦表面上，该装置具有基本上平行于所述第一平坦表面并且距离所述第一平面的第一距离布置的第二平坦表面。 第一平坦表面和所述第二平坦表面以与水平面（HP）大于零度的角度（A）布置。 该装置具有用于供应一定量待应用于所述生物样品的液体的供给装置。 第一平坦表面和第二平坦表面被配置为彼此间隔开第二距离，所述第二距离使得当所述第一平面和所述第二平面是所述第一平面和所述第二平面时，所述供应量的液体分布在所述生物样品上 带到彼此的第二距离。

公开(公告)号：US08673642B2

公开(公告)日：2014-03-18

申请号：US13758810

申请日：2013-02-04

Applicant: DAKO Denmark A/S

Inventor： Marc Key ,  Gordon Feingold ,  Rosanne Welcher

IPC: G01N35/00

CPC classification number: G01N35/00732 ,  G01N1/30 ,  G01N1/31 ,  G01N1/312 ,  G01N35/00712 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N2035/00039 ,  G01N2035/00089 ,  G01N2035/00306 ,  G01N2035/00326 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00435 ,  G01N2035/00881 ,  G01N2035/00891 ,  G16H50/20 ,  Y02A90/22 ,  Y02A90/26 ,  Y10S901/02 ,  Y10S901/41 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/25 ,  Y10T436/2575

Abstract: A sample processing system 101 that may be automated and methods are disclosed where sample(s) 198 are arranged on a carrier element 197 and a process operation control system 171 automatically processes the sample(s) perhaps robotically according to an desired aggregation of event dictated by an input 173. Alteration of an initial aggregated event topology may be accepted while the system is processing an initial aggregation and varied-parameter robotic control simulation functionalities 606 may be accomplished to determine an enhanced sequence for processing. Suggested operator actions may be displayed that might further enhance the scheduling of the altered aggregated event topology together with an automatic operator need prompt 608 that may inform an operator of a need for a particular action in order to accomplish the desired tasks. Reversibility to proposed changes may be made available so that an operator may avoid having to activate proposed changes if they cause a processing result that is not acceptable.

Abstract translation: 公开了可以是自动化的样本处理系统101和方法，其中样本198布置在载体元件197上，并且过程操作控制系统171可以根据事件规定的所需聚合自动地处理样本 可以通过输入173.当系统正在处理初始聚合并且可变参数机器人控制时可以接受初始聚合事件拓扑的改变，可以实现功能606以确定用于处理的增强的序列。 可以显示建议的操作者动作，其可以进一步增强改变的聚合事件拓扑的调度以及可以通知操作者需要特定动作以实现所需任务的自动操作者需求提示608。 提出的改变的可逆性可以提供，以便操作者可以避免在导致不可接受的处理结果时激活所提出的改变。

公开(公告)号：US10655177B2

公开(公告)日：2020-05-19

申请号：US14352815

申请日：2012-10-22

Applicant: DAKO DENMARK A/S

Inventor： Steen Hauge Matthiesen

IPC: C12Q1/6841 ,  C12Q1/6881 ,  C12Q1/6832

Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.

公开(公告)号：US10526646B2

公开(公告)日：2020-01-07

申请号：US15383996

申请日：2016-12-19

Applicant: Dako Denmark A/S

Inventor： Jesper Lohse ,  Mike Paw Maischnack Hansen

IPC: C07D311/82 ,  C12Q1/6841 ,  C07D311/90 ,  C07D493/10 ,  C12N9/08 ,  C12N9/96 ,  C12Q1/28 ,  G01N33/58 ,  C12Q1/68

Abstract: Chromogenic conjugates for color-based detection of targets are described. The conjugates comprise a chromogenic moiety such as rhodamine, rhodol or fluorescein. The chromogenic moiety is linked to a peroxidase substrate. The chromogenic conjugates can be used in immunohistochemical analysis and in situ hybridization. The conjugates can be used to detect 1, 2, 3 or more targets in a sample by color.

公开(公告)号：US10168335B2

公开(公告)日：2019-01-01

申请号：US15165823

申请日：2016-05-26

Applicant: Dako Denmark A/S

Inventor： Jesper Lohse ,  Kenneth Heesche Petersen

IPC: G01N33/58 ,  C12Q1/28 ,  C12Q1/68 ,  C12Q1/6806

Abstract: The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.

公开(公告)号：US20180312912A1

公开(公告)日：2018-11-01

申请号：US15925119

申请日：2018-03-19

Applicant: Applied Biosystems, LLC ,  Dako Denmark A/S

Inventor： Kirsten Vang NIELSEN ,  Jens HYLDIG-NIELSEN ,  Brett F. WILLIAMS

IPC: C12Q1/6832 ,  C12Q1/6876 ,  C12Q1/6837

CPC classification number: C12Q1/6832 ,  C12Q1/6837 ,  C12Q1/6876 ,  C12Q2600/156 ,  C12Q2537/162 ,  C12Q2525/186 ,  C12Q2525/107 ,  C12Q2525/151

Abstract: This invention is directed to methods, kits, non-nucleotide probes as well as other compositions pertaining to the suppression of binding of detectable nucleic acid probes to undesired nucleotide sequences of genomic nucleic acid in assays designed to determine target genomic nucleic acid.

公开(公告)号：US10036066B2

公开(公告)日：2018-07-31

申请号：US14659847

申请日：2015-03-17

Applicant: DAKO DENMARK A/S

Inventor： Lars Winther ,  Jesper Lohse ,  Susanne Gabs ,  Kenneth Heesche Petersen

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6841

CPC classification number: C12Q1/6876 ,  C12Q1/6841 ,  C12Q2525/161 ,  C12Q2565/40

Abstract: The invention provides compositions and methods for the detection of targets in a sample; in particular, an in situ hybridization (ISH) sample. Probes and detectable labels may be provided in multiple layers in order to increase the flexibility of a detection system, and to allow for amplification to enhance the signal from a target. The layers may be created by incorporating probes and detectable labels into larger molecular units that interact through nucleic acids base-pairing, including peptide-nucleic acid (PNA) base-pairing. Optional non-natural bases allow for degenerate base pairing schemes. The compositions and methods are also compatible with immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry, enzyme immuno-assays (EIA), enzyme linked immuno-assays (ELISA), blotting methods (e.g. Western, Southern, and Northern), labeling inside electrophoresis systems or on surfaces or arrays, and precipitation, among other general detection assay formats. The invention is also compatible with many different types of targets, probes, and detectable labels.