公开(公告)号：US20190352592A1

公开(公告)日：2019-11-21

申请号：US16477139

申请日：2018-01-09

Applicant: Calysta, Inc.

Inventor： Luan Thanh NGUYEN ,  Joshua A. Silverman

IPC: C12M1/00 ,  C12N1/30 ,  C12M1/34 ,  C12M1/04

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating in a loop reactor which utilize substantially vertical flow zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US10273446B2

公开(公告)日：2019-04-30

申请号：US15111723

申请日：2015-01-16

Applicant: CALYSTA, INC.

Inventor： Howard Lam Ho Fong ,  John H Grate ,  Luan Nguyen ,  Joshua A. Silverman ,  Lisa Marie Newman ,  Lorraine Joan Giver ,  Drew D. Regitsky

IPC: C12N1/20 ,  B01D53/85 ,  C10L3/10 ,  A23K10/10 ,  A23N17/00 ,  C10G3/00 ,  C10L1/04 ,  C10L1/06 ,  C10L1/08 ,  C12N15/74 ,  C12R1/01 ,  C12N9/02 ,  C12P7/64

Abstract: The present disclosure provides compositions and methods for using recombinant C1 metabolizing microorganisms capable of metabolizing sulfur containing compounds and other contaminants to biologically convert sour or acidic natural gas into high-value molecules, and to allow recovery of stranded oil.

公开(公告)号：US09970032B2

公开(公告)日：2018-05-15

申请号：US14886983

申请日：2015-10-19

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Sol M. Resnick ,  Michael Mendez

IPC: C10L1/02 ,  C10L1/04 ,  C12P7/64 ,  C12N15/74 ,  C12P5/00 ,  C10G3/00 ,  C12N9/16 ,  C12N9/00 ,  C12N1/16 ,  C12N1/20 ,  C12N9/10 ,  C10G47/00 ,  C12N15/52 ,  C12R1/01

CPC classification number: C12P7/6409 ,  C10G3/00 ,  C10G3/50 ,  C10G47/00 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/04 ,  C10L2200/0469 ,  C10L2270/02 ,  C10L2270/04 ,  C10L2290/26 ,  C12N1/16 ,  C12N1/20 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12N15/74 ,  C12P5/00 ,  C12P7/6463 ,  C12P7/649 ,  C12R1/01 ,  C12Y203/01039 ,  C12Y301/02 ,  C12Y602/01003 ,  C12Y604/01002 ,  Y02E50/13 ,  Y02E50/343 ,  Y02P20/52 ,  Y02P30/20

Abstract: The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

公开(公告)号：US20160369246A1

公开(公告)日：2016-12-22

申请号：US14898948

申请日：2014-06-18

Applicant: Sungwon LEE ,  Drew D. REGITSKY ,  Sol M. RESNICK ,  CALYSTA, INC.

Inventor： Renee M. Saville ,  Sungwon Lee ,  Drew D. Regitsky ,  Sol M. Resnick ,  Joshua A. Silverman

IPC: C12N9/04 ,  C07C59/08 ,  C07B59/00 ,  C12P7/56 ,  C12N15/74

CPC classification number: C12N9/0006 ,  C07B59/001 ,  C07B2200/05 ,  C07C59/08 ,  C12N1/20 ,  C12N15/74 ,  C12P7/56 ,  C12Y101/01027 ,  C12Y101/01028

Abstract: The present disclosure provides non-naturally occurring c1 microorganisms useful for the production of lactate and related compositions, as well as methods for the biologically production of lactate. In specific embodiments, the present disclosure provides non-naturally occurring methanotrophic bacteria which are useful for producing lactate from c1 substrates.

Abstract translation: 本公开提供了可用于生产乳酸盐和相关组合物的非天然存在的c1微生物以及用于生物地生产乳酸盐的方法。 在具体实施方案中，本公开提供可用于从c1底物产生乳酸盐的非天然存在的甲烷营养细菌。

公开(公告)号：US20150299745A1

公开(公告)日：2015-10-22

申请号：US14441153

申请日：2013-11-08

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman

IPC: C12P7/64 ,  C12N9/00 ,  C12N9/16 ,  C12N9/04 ,  C12N9/02

CPC classification number: C12P7/6409 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0026 ,  C12N9/0077 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P7/64 ,  C12P7/6436 ,  C12Y101/01 ,  C12Y101/01001 ,  C12Y102/99006 ,  C12Y301/02 ,  C12Y602/01

Abstract: The present disclosure provides compositions and methods for biologically producing fatty acid derivatives, such as fatty alcohols, from recombinant C1 metabolizing microorganisms that utilize C1 substrates such as methane or natural gas as a feedstock.

Abstract translation: 本公开提供了使用C1底物例如甲烷或天然气作为原料的重组C1代谢微生物生物地生产脂肪酸衍生物（例如脂肪醇）的组合物和方法。

公开(公告)号：US20150218508A1

公开(公告)日：2015-08-06

申请号：US14670740

申请日：2015-03-27

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Sol M. Resnick ,  Michael Mendez ,  Renee M. Saville ,  Sungwon Lee ,  Luan Nguyen

IPC: C12N1/20 ,  C12N9/00 ,  C12N9/10 ,  C12N1/16 ,  C12N9/16

CPC classification number: C12P7/6409 ,  C10G3/00 ,  C10G3/50 ,  C10G47/00 ,  C10G2300/1014 ,  C10L1/02 ,  C10L1/04 ,  C10L2200/0469 ,  C10L2270/02 ,  C10L2270/04 ,  C10L2290/26 ,  C12N1/16 ,  C12N1/20 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12N15/74 ,  C12P5/00 ,  C12P7/6463 ,  C12P7/649 ,  C12R1/01 ,  C12Y203/01039 ,  C12Y301/02 ,  C12Y602/01003 ,  C12Y604/01002 ,  Y02E50/13 ,  Y02E50/343 ,  Y02P20/52 ,  Y02P30/20

Abstract: The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C1 metabolizing microorganism reactor system for converting C1 substrates, such as methane or methanol, into biomass and subsequently into biofuels, bioplastics, or the like.

公开(公告)号：US20150087036A1

公开(公告)日：2015-03-26

申请号：US14344899

申请日：2012-10-17

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Tom Purcell ,  Jon Edward Ness ,  Effendi Leonard

IPC: C12P7/42 ,  C12P7/40

CPC classification number: C12P7/42 ,  C12N15/52 ,  C12P7/40 ,  C12P19/32

Abstract: Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.

Abstract translation: 本文提供的是不能将丙酰辅酶A转化为甲基丙二酰辅酶A或2-甲基柠檬酸合酶的烷烃代谢细胞。 根据细胞中哪些酶的存在，细胞可以生成丙烯酸酯或相同的前体（例如，丙酸酯，3-羟基丙酰基-CoA，3-羟基丙酸酯，丙烯酰辅酶A），其可以容易地酶转化成丙烯酸酯（例如 ，在细胞中）或通过化学处理。 在一个实施方案中，细胞可以含有细胞色素P450或烷烃氧化酶，其允许产生3-羟基丙酰辅酶A，其可以容易地转化为3-羟基丙酸酯。 为了制备这种化合物，可以在奇数链烷烃（例如戊烷或庚烷）的存在下生长电池，尽管可以使用另一个奇数链烷烃。 在另一个实施方案中，细胞可以含有酰基辅酶A氧化酶，烯酰辅酶A水合酶和水解酶。

公开(公告)号：US11939567B2

公开(公告)日：2024-03-26

申请号：US17513094

申请日：2021-10-28

Applicant: Calysta, Inc.

Inventor： Luan Thanh Nguyen ,  Joshua A. Silverman ,  Graham Ian Aylen

IPC: B01D19/00 ,  C12M1/00 ,  C12M1/107 ,  C12M1/12

CPC classification number: C12M45/04 ,  B01D19/00 ,  B01D19/0042 ,  C12M21/04 ,  C12M21/12 ,  C12M23/06 ,  C12M29/18 ,  C12M29/20

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US20220010268A1

公开(公告)日：2022-01-13

申请号：US17293832

申请日：2019-12-26

Applicant: Calysta, Inc.

Inventor： Renee M. Saville ,  Joshua A. Silverman ,  Vincent Tang ,  Eric G. Luning ,  Paloma Rueda ,  Megan Hsi ,  Yelena Stegantseva

IPC: C12N1/32 ,  C12N9/12 ,  C12N9/10 ,  C12N9/90 ,  C12P21/00 ,  C12P13/04 ,  C12N1/30

Abstract: The present disclosure provides methanotrophic bacteria that are modified to produce less glycogen, and methods of using the modified methanotrophic bacteria to produce a desired product, such as protein(s) or metabolite(s).

公开(公告)号：US10889842B2

公开(公告)日：2021-01-12

申请号：US14599383

申请日：2015-01-16

Applicant: CALYSTA, INC.

Inventor： Renee M. Saville ,  Joshua A. Silverman ,  Eric G. Luning ,  Brandon D. Doss ,  Lorraine Joan Giver ,  Sol M. Resnick ,  Drew D. Regitsky

IPC: C12N1/04 ,  C12N1/32 ,  C12P13/08 ,  C12P13/12 ,  C12N15/52 ,  C12N1/30

Abstract: The present disclosure relates to recombinant microorganisms engineered for enhance production of a desired amino acid, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present invention also provides related methods.