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公开(公告)号：US20240226357A1
公开(公告)日：2024-07-11
申请号：US18604231
申请日：2024-03-13
Applicant: Cytiva BioProcess R&D AB
Inventor： Elin Marianne Monie , Tomas Bjorkman , Anna Gronberg , Anders Ljunglof , Gustav Jose Rodrigo , Karin Torstenson , Magnus Carl Erik Wetterhall
CPC classification number: A61L2/18 , B01D15/203 , B01D15/3804 , G01N30/50
Abstract: The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of:
a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support,
b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I,
wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.-
公开(公告)号：US11685764B2
公开(公告)日：2023-06-27
申请号：US17221438
申请日：2021-04-02
Applicant: Cytiva BioProcess R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: A23J1/00 , C07K1/22 , B01D15/38 , B01J20/24 , B01J20/28 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/06 , C07K17/10
CPC classification number: C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/286 , B01J20/28016 , B01J20/28078 , B01J20/3212 , B01J20/3274 , C07K14/31 , C07K16/00 , C07K16/065 , C07K17/10
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein:
a) the ligands comprise multimers of alkali-stabilized Protein A domains, and
b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50, v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.-
Patent Agency Ranking
公开(公告)号：US11680081B2
公开(公告)日：2023-06-20
申请号：US16955119
申请日：2018-12-13
Applicant: Cytiva Bioprocess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Ronnie Palmgren , Tomas Bjorkman
CPC classification number: C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/288 , B01J20/28016 , B01J20/3071 , B01J20/3212 , B01J20/3217 , B01J20/3274 , B01J20/3295 , C07K16/00 , B01J2220/54
Abstract: Methods that include providing and reacting a solid support and an alkali-stable ligand derived from an immunoglobulin-binding bacterial protein to form a separation matrix having covalently coupled alkali-stable ligands; and washing with a wash solution comprising at least 10 mM of an alkali metal hydroxide.
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公开(公告)号：US11623941B2
公开(公告)日：2023-04-11
申请号：US16884475
申请日：2020-05-27
Applicant: Cytiva BioProcess R&D AB
Inventor： Annika Kristina Forss , Gustav Jose Rodrigo , Tomas Bjorkman , Jesper Ulf Hansson , Mats Ander
IPC: C07K1/22 , B01D15/08 , C07K16/12 , C07K17/10 , B01J20/286 , C07K14/31 , C07K16/06 , B01D15/00 , B01J20/32
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20220362744A1
公开(公告)日：2022-11-17
申请号：US17853514
申请日：2022-06-29
Applicant: Cytiva BioProcess R&D AB
Inventor： Ronnie Palmgren , Jean-Luc Maloisel , Gustav Rodrigo , Tomas Bjorkman
Abstract: A separation matrix comprising porous particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is above 5 mg/ml, the volume-weighted median diameter of said porous particles is at least 10 and below 30 μm and the said porous particles have a gel phase distribution coefficient, expressed as KD for dextran of molecular weight 110 kDa, of 0.5-0.9.
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公开(公告)号：US20210221841A1
公开(公告)日：2021-07-22
申请号：US17221438
申请日：2021-04-02
Applicant: Cytiva BioProcess R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , B01D15/38 , B01J20/24 , B01J20/28 , B01J20/286 , B01J20/32 , C07K14/31 , C07K16/00 , C07K16/06 , C07K17/10
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
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公开(公告)号：US20210147577A1
公开(公告)日：2021-05-20
申请号：US17149830
申请日：2021-01-15
Applicant: Cytiva BioProcess R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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