公开(公告)号：US08426205B2

公开(公告)日：2013-04-23

申请号：US13555946

申请日：2012-07-23

Applicant: Peter Eriksson ,  Daniel T. Chiu ,  Alexander Moscho ,  Owe Orwar ,  Richard N. Zare

Inventor： Peter Eriksson ,  Daniel T. Chiu ,  Alexander Moscho ,  Owe Orwar ,  Richard N. Zare

IPC: C12N15/06

CPC classification number: C12N15/87 ,  C12N5/12 ,  C12N13/00

Abstract: Disclosed is a method for selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising the following steps: A) the fusion partners are brought into contact with each other and B) an electrical field of a strength sufficient to obtain fusion and highly focused on the fusion partners is applied. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.

Abstract translation: 公开了一种用于选择性电融合具有细胞样膜和细胞或亚细胞尺寸的至少两个融合伴侣的方法，包括以下步骤：A）使融合伴侣彼此接触并且B）强度的电场 足以获得融合并高度集中在融合伙伴上。 融合对象独立地选自单细胞，脂质体，蛋白脂质体，合成囊泡，卵细胞，去核卵细胞，任何发育阶段的精细胞和植物原生质体。

公开(公告)号：US20120294903A1

公开(公告)日：2012-11-22

申请号：US13457609

申请日：2012-04-27

Applicant: Peng Guo ,  Charles R. Martin ,  Yaping Zhao ,  Richard N. Zare

Inventor： Peng Guo ,  Charles R. Martin ,  Yaping Zhao ,  Richard N. Zare

IPC: B01J19/24 ,  A61K47/36 ,  A61K47/42 ,  A61K35/12 ,  A61K9/00 ,  C08B37/08 ,  A61P19/00 ,  B82Y40/00 ,  B82Y5/00

CPC classification number: A61K9/5161 ,  A61K9/70 ,  B01J19/2475 ,  B01J2219/00177 ,  B82Y5/00 ,  B82Y40/00 ,  C08L5/08

Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making nanostructures (e.g., nanoparticles, nanofibers), systems for making nanostructures, and the like.

Abstract translation: 根据本公开的目的，如本文所体现和广泛描述的，本公开的实施方案在一个方面涉及制备纳米结构（例如纳米颗粒，纳米纤维），制备纳米结构的体系和 类似。

公开(公告)号：US08285367B2

公开(公告)日：2012-10-09

申请号：US11973367

申请日：2007-10-05

Applicant: Roderick A. Hyde ,  Edward K. Y. Jung ,  Nathan P. Myhrvold ,  Clarence T. Tegreene ,  Willard H. Wattenburg ,  Lowell L. Wood, Jr. ,  Richard N. Zare

Inventor： Roderick A. Hyde ,  Edward K. Y. Jung ,  Nathan P. Myhrvold ,  Clarence T. Tegreene ,  Willard H. Wattenburg ,  Lowell L. Wood, Jr. ,  Richard N. Zare

IPC: A61B6/00

CPC classification number: A61B5/0205 ,  A61B1/041 ,  A61B1/043 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0095 ,  A61B5/02007 ,  A61B5/0215 ,  A61B5/026 ,  A61B5/0295 ,  A61B5/061 ,  A61B5/14539 ,  A61B5/14546 ,  A61B5/412 ,  A61B5/418 ,  A61B5/6862 ,  A61B5/6876 ,  A61B5/7267 ,  A61B8/12 ,  A61B8/56

Abstract: In an embodiment, a system includes one or one or more reservoirs responsive to control circuitry for receiving target(s) associated with one or more blood or lymph vessel of a subject. The system includes one or more first energy source associated with the one or more reservoirs for providing energy to elicit image response(s) associated with the target(s). The system includes one or more sensors associated with the one or more reservoirs for capturing the image response(s). Control circuitry is provided and coupled to the sensors for at least partially identifying the target(s) based at least partially on the captured image response(s) during an expected transit time of the target(s) through a detection area. Attachment unit(s) responsive to the control circuitry are provided for attaching the one or more reservoirs to the blood or lymph vessel.

Abstract translation: 在一个实施例中，系统包括响应于控制电路的一个或多个储存器，用于接收与受试者的一个或多个血液或淋巴管相关联的目标。 该系统包括与一个或多个储存器相关联的一个或多个第一能量源，用于提供能量以引起与目标相关联的图像响应。 该系统包括与用于捕获图像响应的一个或多个储存器相关联的一个或多个传感器。 控制电路被提供并耦合到传感器，用于至少部分地基于在目标通过检测区域的预期传播时间内的捕获图像响应来识别目标。 提供响应于控制电路的附接单元，用于将一个或多个储存器附接到血液或淋巴管。

公开(公告)号：US08165663B2

公开(公告)日：2012-04-24

申请号：US11973010

申请日：2007-10-03

Applicant: Roderick A. Hyde ,  Edward K. Y. Jung ,  Nathan P. Myhrvold ,  Clarence T. Tegreene ,  Willard H. Wattenburg ,  Lowell L. Wood, Jr. ,  Richard N. Zare

Inventor： Roderick A. Hyde ,  Edward K. Y. Jung ,  Nathan P. Myhrvold ,  Clarence T. Tegreene ,  Willard H. Wattenburg ,  Lowell L. Wood, Jr. ,  Richard N. Zare

IPC: A61B6/00

CPC classification number: A61B5/0071 ,  A61B1/041 ,  A61B1/043 ,  A61B1/3137 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/02007 ,  A61B5/053 ,  A61B5/412 ,  A61B5/417 ,  A61B5/418 ,  A61B5/7267 ,  A61B8/12 ,  A61B8/56 ,  A61B18/20 ,  A61B18/22 ,  A61B2017/00106 ,  A61B2018/1807 ,  A61N7/02

Abstract: In an embodiment, an untethered device includes one or more first energy sources configured to provide energy to elicit one or more image responses associated with one or more blood vessels or lymph vessels. The device includes one or more sensors configured to capture the one or more image responses. Control circuitry is provided and coupled to the sensors for at least partially identifying one or more targets at least partially based on the captured one or more image responses. One or more inserts are provided, which are configured to be disposed in the one or more blood vessels or lymph vessels, and slow or trap the blood vessels or lymph vessels therein. One or more second energy sources are provided for ablating the one or more targets when they are slowed or trapped by the one or more inserts.

Abstract translation: 在一个实施例中，无束缚装置包括被配置为提供能量以引发与一个或多个血管或淋巴管相关联的一个或多个图像响应的一个或多个第一能量源。 该装置包括配置成捕获一个或多个图像响应的一个或多个传感器。 控制电路被提供并且耦合到传感器，用于至少部分地基于所捕获的一个或多个图像响应部分地识别一个或多个目标。 提供了一个或多个插入物，其被配置为设置在一个或多个血管或淋巴管中，并且缓慢或捕获其中的血管或淋巴管。 提供一个或多个第二能量源，用于当一个或多个靶被减速或被一个或多个插入物捕获时，用于烧蚀一个或多个靶。

公开(公告)号：US20110186818A1

公开(公告)日：2011-08-04

申请号：US12800072

申请日：2010-05-07

Applicant: Jeffrey A. Bowers ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  Clarence T. Tegreene ,  Tatsushi Toyokuni ,  Richard N. Zare

Inventor： Jeffrey A. Bowers ,  Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Jordin T. Kare ,  Clarence T. Tegreene ,  Tatsushi Toyokuni ,  Richard N. Zare

IPC: H01L29/12 ,  H01L21/04 ,  H01L29/06

CPC classification number: H01L29/167 ,  H01L21/04 ,  H01L29/06 ,  H01L29/12 ,  H01L29/1606 ,  H01L29/36

Abstract: A graphene substrate is doped with one or more functional groups to form an electronic device.

公开(公告)号：US20100104834A1

公开(公告)日：2010-04-29

申请号：US12378086

申请日：2009-02-09

Applicant: Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Edward K.Y. Jung ,  Jordin T. Kare ,  Alois A. Langer ,  Eric C. Leuthardt ,  Nathan P. Myhrvold ,  Thomas J. Nugent, JR. ,  Clarence T. Tegreene ,  Charles Whitmer ,  Lowell L. Wood, JR. ,  Richard N. Zare

Inventor： Roderick A. Hyde ,  Muriel Y. Ishikawa ,  Edward K.Y. Jung ,  Jordin T. Kare ,  Alois A. Langer ,  Eric C. Leuthardt ,  Nathan P. Myhrvold ,  Thomas J. Nugent, JR. ,  Clarence T. Tegreene ,  Charles Whitmer ,  Lowell L. Wood, JR. ,  Richard N. Zare

IPC: B32B7/00

CPC classification number: C08J9/40 ,  Y10T428/24802 ,  Y10T428/24917

Abstract: Articles having porous or foam-like elements are provided. The design, fabrication and structures of the articles exploit properties of reactive composite materials (RCM) and their reaction products. In particular, fluids generated by reacting RCM are utilized to create or fill voids in the porous or foam-like elements.

公开(公告)号：US20100055802A1

公开(公告)日：2010-03-04

申请号：US12584345

申请日：2009-09-02

Applicant: Richard N. Zare ,  Eric R. Crosson ,  Bruce A. Richman ,  Nabil M.R. Saad

Inventor： Richard N. Zare ,  Eric R. Crosson ,  Bruce A. Richman ,  Nabil M.R. Saad

IPC: G01N21/75 ,  G01N21/00 ,  G01N30/74 ,  G01N27/26

CPC classification number: G01N21/39 ,  G01N30/74

Abstract: The present invention relates to the separation, quantitative measurement, and analysis of trace species using a combination of three steps in succession. First, trace species are separated from other species that are present. Second, the trace species are chemically modified to convert them into specific species that are advantageous for the third and final step. In this last step, cavity enhanced optical detection of the converted species is performed to detect and measure the concentrations of the species of interest. Because the last step has spectroscopic resolution, the concentration of isotopologues in each converted species can be determined. Further processing can provide the ratios between pairs of isotopologues, in particular the ratio of the rare isotopologues to the most abundant isotopologue.

Abstract translation: 本发明涉及使用三个步骤的组合的痕量物质的分离，定量测量和分析。 首先，痕量物种与存在的其他物种分离。 第二，痕量物质被化学修饰，以将它们转化成对于第三和最后步骤有利的特定物种。 在最后一步中，进行转化物种的腔增强光学检测以检测和测量感兴趣物种的浓度。 由于最后一步具有光谱分辨率，因此可以确定每个转化物种中同位素的浓度。 进一步的处理可以提供成对的同位素异构体之间的比率，特别是罕见的同位素异构体与最丰富的同位素的比例。

公开(公告)号：US20070180693A1

公开(公告)日：2007-08-09

申请号：US11701697

申请日：2007-02-02

Applicant: Oh-Kyu Yoon ,  Richard N. Zare

Inventor： Oh-Kyu Yoon ,  Richard N. Zare

IPC: H01J49/00

CPC classification number: H01J49/061 ,  Y10T29/4902 ,  Y10T29/49117

Abstract: A Bradbury-Nielson gate (BNG) includes a set of evenly spaced, co-planar, and parallel wires. The wires alternate in a repeating ABAB pattern, where all of the A wires are electrically connected to each other, all of the B wires are electrically connected to each other, and the set of A wires is electrically isolated from the set of B wires. Improved fabrication of Bradbury-Nielson gates is provided based on two key ideas. The first key idea is the use of wire positioning template surfaces having wire insertion features with enhanced spacing. Wire insertion features having enhanced spacing allow for non-microscopic assembly of finely spaced wire arrays. The second key idea is the use of two template surfaces, each having wires spaced by twice the eventual gate wire spacing. The use of two template surfaces facilitates making the alternating electrical contact required for a BNG.

Abstract translation: Bradbury-Nielson门（BNG）包括一组均匀间隔的共面平行线。 导线以重复的ABAB图案交替，其中所有的A线彼此电连接，所有B线彼此电连接，并且该组A线与该组B线电隔离。 基于两个关键思想提供了改进Bradbury-Nielson门的制作。 第一个关键思想是使用具有增强间距的线插入特征的线定位模板表面。 具有增强的间距的电线插入特征允许精细间隔的线阵列的非微观组装。 第二个关键思想是使用两个模板表面，每个模板表面具有间隔两倍于最终栅极间距的导线。 使用两个模板表面有助于实现BNG所需的交流电接触。

公开(公告)号：US06664545B2

公开(公告)日：2003-12-16

申请号：US10230606

申请日：2002-08-28

Applicant: Joel R. Kimmel ,  Friedrich Engelke ,  Richard N. Zare

Inventor： Joel R. Kimmel ,  Friedrich Engelke ,  Richard N. Zare

IPC: H01J146

CPC classification number: H01J49/061 ,  H01J9/14

Abstract: Bradbury-Nielson gates for the modulation of beams of charged particles, particularly ion beams in mass spectrometry, have been produced with an adjustable wire spacing down to 0.075 mm or a smaller spacing. The gates are robust, they can be fabricated in less than 3 hours, and the method of production is reproducible. In time-of-flight mass spectrometers, fine wire spacing leads to improvements in mass resolution and modulation rates. Gates that were produced using this new method have been installed in a Hadamard transform time-of-flight mass spectrometer in order to demonstrate their utility.

Abstract translation: 已经制造了用于调制带电粒子束，特别是质谱中的离子束的Bradbury-Nielson门，其可调节的线间距可以降到0.075mm或更小的间隔。 门是坚固的，它们可以在不到3小时内制造，并且生产方法是可重现的。 在飞行时间质谱仪中，细线间距导致质量分辨率和调制率的提高。 使用这种新方法生产的门已经安装在Hadamard变换飞行时间质谱仪中，以证明其实用性。

公开(公告)号：US5141621A

公开(公告)日：1992-08-25

申请号：US470883

申请日：1990-01-26

Applicant: Richard N. Zare ,  Takao Tsuda

Inventor： Richard N. Zare ,  Takao Tsuda

IPC: G01N27/447

CPC classification number: G01N27/44743

Abstract: An apparatus and method useful for sample injection in capillary electrophoresis is disclosed. The apparatus comprises an interface device with capillary and tubing inserted therein. Sample is injected through the tubing into the interface and is thereafter introduced into the capillary column in which the capillary electrophoresis separation is performed. The apparatus introduces precise amounts of sample into the capillary and the interface can be designed for split flow or direct sample introduction. Employing the interface device, the injection method permits samples to be introduced into capillaries without the need to disengage or to alter the electric field. The method is suited for adaptation with autosamplers or auto-injection systems.

Abstract translation: 公开了一种用于毛细管电泳中样品注入的装置和方法。 该装置包括一个插入其中的毛细管和管道的接口装置。 将样品通过管道注入界面，然后将其引入进行毛细管电泳分离的毛细管柱中。 该设备将精确量的样品引入毛细管中，并且界面可以设计用于分流或直接样品引入。 使用接口装置，注射方法允许将样品引入毛细管中，而不需要脱离或改变电场。 该方法适用于自动进样器或自动注射系统。