公开(公告)号：US20210248746A1

公开(公告)日：2021-08-12

申请号：US17174919

申请日：2021-02-12

Applicant: SamanTree Medical SA

Inventor： Andrey Naumenko ,  Aurèle Timothée Horisberger ,  Diego Joss ,  Frédéric Schmitt ,  Etienne Shaffer ,  Jonathan Abel Pirolet ,  Bastien Rachet ,  Andrew Logvinov

IPC: G06T7/00 ,  G02B21/00 ,  G01N33/483

Abstract: Systems and methods to identify and/or reduce or eliminate sample motion artifacts are disclosed. Sample motion artifacts may be reduced or eliminated using scan patterns where an acquisition time difference between when perimeter pixels in adjacent tiles are acquired is reduced, as compared to a conventional raster scan to reduce or eliminate discontinuities that would otherwise appear at tile boundaries in an image. In some embodiments, test images acquired using relatively small test scan patterns or intensities of test points acquired at different times may be compared to determine whether sample motion has occurred. In some embodiments, intensity of adjacent pixels at a tile boundary are compared. In some embodiments, intensity of one or more single pixels is monitored over time to determine whether sample motion has occurred over a period of time. In some embodiments, a flattening or reshaping tool may be used to suppress sample motion during imaging.

公开(公告)号：US20210124162A1

公开(公告)日：2021-04-29

申请号：US17141933

申请日：2021-01-05

Applicant: SamanTree Medical SA

Inventor： Etienne Shaffer ,  Jonathan Abel Pirolet ,  Frédéric Schmitt ,  Bastien Rachet ,  Diego Joss ,  Aurèle Timothée Horisberger

IPC: G02B21/34 ,  G01N21/01 ,  G01N21/64 ,  G02B21/36

Abstract: Disclosed herein are sample dishes for use with microscopes that are simple to mount on a microscope and facilitate easy manipulation of tissue samples disposed thereon during imaging as well as methods of their use. A sample dish comprises an optical interface and, optionally, a support member that holds the optical interface. The optical interface of a sample dish is suitably transparent and planar such that a focal plane of a microscope can reside uniformly at or within a surface of a sample during imaging. In certain embodiments, a support member comprises a dish for holding excess fluid. In certain embodiments, a sample dish comprises separation ribs. In certain embodiments, a sample dish comprises one or more manipulation members (e.g., tabs). In certain embodiments, a sample dish is used with an imaging artifact reducing fluid.

公开(公告)号：US20200257098A1

公开(公告)日：2020-08-13

申请号：US16862497

申请日：2020-04-29

Applicant: SamanTree Medical SA

Inventor： Etienne Shaffer ,  Bastien Rachet ,  Aurèle Timothée Horisberger ,  Jonathan Abel Pirolet ,  Diego Joss ,  Andrey Naumenko ,  Frédéric Schmitt

IPC: G02B21/00 ,  G02B21/36 ,  G02B21/26 ,  G01N21/64

Abstract: Disclosed herein are systems for imaging of samples, for example, using an array of micro optical elements and methods of their use. In some embodiments, an optical chip comprising an array of micro optical elements moves relative to an imaging window and a detector in order to scan over a sample to produce an image. A focal plane can reside within a sample or on its surface during imaging. In some embodiments, detecting optics a reused to detect back-emitted light collected by an array of micro optical elements that is generated by an illumination beam impinging on a sample. In some embodiments, an imaging system has a large field of view and a large optical chip such that an entire surface of a sample can be imaged quickly. In some embodiments, a sample is accessible by a user during imaging due to the sample being exposed.

公开(公告)号：US20190137752A1

公开(公告)日：2019-05-09

申请号：US16146695

申请日：2018-09-28

Applicant: SamanTree Medical SA

Inventor： Etienne Shaffer ,  Bastien Rachet ,  Aurèle Timothée Horisberger ,  Jonathan Abel Pirolet ,  Diego Joss ,  Andrey Naumenko ,  Frédéric Schmitt

IPC: G02B21/36 ,  G02B21/00 ,  G02B21/08 ,  G02B21/16 ,  G01N21/64 ,  A61B90/20 ,  H04N5/225 ,  H04N7/18

CPC classification number: G02B21/367 ,  A61B90/20 ,  G01N21/6428 ,  G01N2021/6439 ,  G02B21/0012 ,  G02B21/0024 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/082 ,  G02B21/16 ,  H04N5/2256 ,  H04N7/18

Abstract: Disclosed herein are systems for imaging of samples using an array of micro optical elements and methods of their use. In some embodiments, an optical chip comprising an array of micro optical elements moves relative to an imaging window and a detector in order to scan over a sample to produce an image. A focal plane can reside within a sample or on its surface during imaging. Detecting optics are used to detect back-emitted light collected by an array of micro optical elements that is generated by an illumination beam impinging on a sample. In some embodiments, an imaging system has a large field of view and a large optical chip such that an entire surface of a sample can be imaged quickly. In some embodiments, a sample is accessible by a user during imaging due to the sample being exposed while disposed on or over an imaging window.

公开(公告)号：US20180348142A1

公开(公告)日：2018-12-06

申请号：US16054681

申请日：2018-08-03

Applicant: SamanTree Medical SA

Inventor： Bastien Rachet ,  Davor Kosanic ,  Etienne Shaffer

IPC: G01N21/77 ,  G02B21/36 ,  G01N21/64 ,  G02B21/26 ,  G02B21/34 ,  G01N33/483 ,  G02B21/00 ,  G02B21/16 ,  G02B21/24

CPC classification number: G01N21/77 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/4833 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2021/7786 ,  G01N2201/0633 ,  G01N2201/068 ,  G02B21/0012 ,  G02B21/0032 ,  G02B21/004 ,  G02B21/0044 ,  G02B21/0076 ,  G02B21/0088 ,  G02B21/16 ,  G02B21/24 ,  G02B21/26 ,  G02B21/34 ,  G02B21/361

Abstract: The disclosed technology brings histopathology into the operating theatre, to enable real-time intra-operative digital pathology. The disclosed technology utilizes confocal imaging devices image, in the operating theatre, “optical slices” of fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital histology scanner, with the capability of rapidly scanning all outer margins of a tissue sample (e.g., resection lump, removed tissue mass). Using point-scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the tissue sample itself.

公开(公告)号：US12236603B2

公开(公告)日：2025-02-25

申请号：US17174919

申请日：2021-02-12

Applicant: SamanTree Medical SA

Inventor： Andrey Naumenko ,  Aurèle Timothée Horisberger ,  Diego Joss ,  Frédéric Schmitt ,  Etienne Shaffer ,  Jonathan Abel Pirolet ,  Bastien Rachet ,  Andrew Logvinov

IPC: G06T7/00 ,  G01N33/483 ,  G02B21/00 ,  G02B21/36 ,  G06T3/4038

Abstract: Systems and methods to identify and/or reduce or eliminate sample motion artifacts are disclosed. Sample motion artifacts may be reduced or eliminated using scan patterns where an acquisition time difference between when perimeter pixels in adjacent tiles are acquired is reduced, as compared to a conventional raster scan to reduce or eliminate discontinuities that would otherwise appear at tile boundaries in an image. In some embodiments, test images acquired using relatively small test scan patterns or intensities of test points acquired at different times may be compared to determine whether sample motion has occurred. In some embodiments, intensity of adjacent pixels at a tile boundary are compared. In some embodiments, intensity of one or more single pixels is monitored over time to determine whether sample motion has occurred over a period of time. In some embodiments, a flattening or reshaping tool may be used to suppress sample motion during imaging.

公开(公告)号：US11609186B2

公开(公告)日：2023-03-21

申请号：US16054681

申请日：2018-08-03

Applicant: SamanTree Medical SA

Inventor： Bastien Rachet ,  Davor Kosanic ,  Etienne Shaffer

IPC: G02B21/00 ,  G01N21/77 ,  G02B21/26 ,  G02B21/34 ,  G02B21/36 ,  G01N21/64 ,  G01N33/483 ,  G02B21/16 ,  G02B21/24

Abstract: The disclosed technology brings histopathology into the operating theatre, to enable real-time intra-operative digital pathology. The disclosed technology utilizes confocal imaging devices image, in the operating theatre, “optical slices” of fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital histology scanner, with the capability of rapidly scanning all outer margins of a tissue sample (e.g., resection lump, removed tissue mass). Using point-scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the tissue sample itself.

公开(公告)号：US20220269059A1

公开(公告)日：2022-08-25

申请号：US17739612

申请日：2022-05-09

Applicant: SamanTree Medical SA

Inventor： Etienne Shaffer ,  Jonathan Abel Pirolet ,  Frédéric Schmitt ,  Bastien Rachet ,  Diego Joss ,  Aurèle Timothée Horisberger

IPC: G02B21/34 ,  G02B21/36 ,  G01N21/64 ,  G01N21/01

Abstract: Disclosed herein are sample dishes for use with microscopes that are simple to mount on a microscope and facilitate easy manipulation of tissue samples disposed thereon during imaging as well as methods of their use. A sample dish comprises an optical interface and, optionally, a support member that holds the optical interface. The optical interface of a sample dish is suitably transparent and planar such that a focal plane of a microscope can reside uniformly at or within a surface of a sample during imaging. In certain embodiments, a support member comprises a dish for holding excess fluid. In certain embodiments, a sample dish comprises separation ribs. In certain embodiments, a sample dish comprises one or more manipulation members (e.g., tabs). In certain embodiments, a sample dish is used with an imaging artifact reducing fluid.

公开(公告)号：US20200284703A1

公开(公告)日：2020-09-10

申请号：US16806555

申请日：2020-03-02

Applicant: SamanTree Medical SA

Inventor： Etienne Shaffer ,  Aurèle Timothée Horisberger ,  Frédéric Schmitt ,  Bastien Rachet

IPC: G01N1/30 ,  C09K11/06

Abstract: Disclosed herein are staining agent solutions for use in fluorescence microscopy. One example of fluorescence microscopy in which the staining agent solutions are useful is intraoperative confocal microscopy. Intraoperative confocal microscopy can be used, for example, to assess margins of resected cancerous tissue. Example staining agent solutions include acridine orange, where the pH of the solution is from 6 to 8, for example from 6.8 to 7.4. The solution may include a phosphate-buffered saline (PBS). The solution may be isotonic. The solution may comprise one or more of potassium dihydrogen phosphate, disodium hydrogen phosphate, and sodium chloride. In some embodiments, the staining agent solution does not comprise acetic acid (e.g., an acetate buffer). Staining agent solutions disclosed herein may reduce adverse impacts to tissue resulting from staining. Staining agent solutions disclosed herein may preserve normal appearance of tissue. Staining agent solutions disclosed herein may have negligible or reduced odor.

公开(公告)号：US20200033581A1

公开(公告)日：2020-01-30

申请号：US16593726

申请日：2019-10-04

Applicant: SamanTree Medical SA

Inventor： Etienne Shaffer ,  Bastien Rachet ,  Aurèle Timothée Horisberger ,  Jonathan Abel Pirolet ,  Diego Joss ,  Andrey Naumenko ,  Frédéric Schmitt

IPC: G02B21/36 ,  G02B21/00 ,  A61B90/20 ,  G01N21/64 ,  G02B21/08 ,  G02B21/16 ,  H04N5/225 ,  H04N7/18

Abstract: Disclosed herein are systems for imaging of samples using an array of micro optical elements and methods of their use. In some embodiments, an optical chip comprising an array of micro optical elements moves relative to an imaging window and a detector in order to scan over a sample to produce an image. A focal plane can reside within a sample or on its surface during imaging. Detecting optics are used to detect back-emitted light collected by an array of micro optical elements that is generated by an illumination beam impinging on a sample. In some embodiments, an imaging system has a large field of view and a large optical chip such that an entire surface of a sample can be imaged quickly. In some embodiments, a sample is accessible by a user during imaging due to the sample being exposed while disposed on or over an imaging window.