Abstract:
A timing system for a spectrophotometer having a line driven chopper including a rotating disc with alternate open and opaque segments, index apertures in the disc spaced at invariant angular relationship to one of the open segments for providing indexing pulses having fixed time relationship to the light segments, and a programmable microcomputer assembly for generating a string of timing pulses for timing spectrophotometer operations and adjusting the rate of the timing pulses to provide a fixed number between indexing pulses in fixed phase relationship thereto.
Abstract:
Variations in the intensity of a light source and the sensitivity of a fluorescence detector photo-multiplier tube are simultaneously compensated by measuring the intensity (I.sub.LS1) of the light source at the sample excitation frequency with a silicon detector, measuring the intensity (I.sub.LS2) of the light source at the emission frequency with another silicon detector, measuring with the photo-multiplier detector the intensity (I.sub.S) of the sample's fluorescence and the intensity (I.sub.LS3) of the light source at the emission frequency of the sample and then calculating a value F as representative of the sample's fluorescence according to the formula: ##EQU1##
Abstract:
A detector for use in optical measuring instruments such as a double-beam spectrophotometer, comprising a photoelectric element having a photosensitive surface and a beam mixer disposed adjacent the photosensitive surface for causing each of two alternate light beams to be projected evenly and uniformly onto the photosensitive surface of the photoelectric element thereby to eliminate errors which would otherwise be caused in the result of measurement due to local difference in sensitivity of the photosensitive surface of the photoelectric element. The beam mixer comprises a symmetrical pair of light diffusing plates so arranged as to form a roof-shaped configuration and a tublar body having one end opening closed by the pair of light diffusing plates and the opposite end opening closed by the photosensitive surface of the photoelectric element.
Abstract:
A cuvette for liquid chromatographic analysis and like determinations having a chamber through which the liquid being analyzed flows, a single optical body for directing exciting light to the chamber and emitted light to a detector and means for holding the optical body in intimate contact with the chamber.
Abstract:
Multi-channel analyzer for the optical comparison of sample fractions separated by liquid chromatography for detecting differences or ratios between, for example, reference and sample, solvent and sample, buffer and sample, or two different samples, having a single light source, a single dispersing element and a single collimating, refocusing optic for each spectrum produced, wherein the reference and sample passing through flow cuvettes are exposed and the ratio or difference between the optical characteristics of the reference and the sample are read.
Abstract:
There is disclosed a light modulating structure including an open-ended housing within which an electromagnetically driven tuning fork structure is supported. A source of light is located between the tines of the tuning fork. Apertured shutter members are secured to the ends of said tines intermediate the light source and the open end of said housing, relative movement of the shutter members causing variations in light intensity emitted by the light modulating structure. The tuning fork is of composite construction and is manufactured by a method which involves shaping at least two tuning fork tines to a desired thickness in a single machining operation before the two tines are separated and joined together symmetrically to form a composite tuning fork.
Abstract:
A synchronizing light chopper which is rotatable and has a mechanical member in the form of a cylinder with a multiplicity of slots cut therethrough. One of the slots is wider than the others and permits a longer light pulse therein which is used to reset the flip-flop to its initial zero state. Thus, synchronization is ensured in spite of spurious, unwanted trigger pulses.