公开(公告)号：US08273310B2

公开(公告)日：2012-09-25

申请号：US11848748

申请日：2007-08-31

Applicant: Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

Inventor： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park

IPC: B04B13/00

CPC classification number: B01L3/502738 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/0233 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0076 ,  F16K2099/0084

Abstract: A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves.

Abstract translation: 提供了用于核酸提取的离心力微流体装置和微流体系统。 微流体装置包括一个革命体; 设置在旋转体内的微流体结构，所述微流体结构包括多个室，连接所述室的通道和设置在通道中的阀以控制流体流动，所述微流体结构由于所述体的旋转而使用离心力传递流体 的革命 以及包含在其中一个室中的磁珠，其从流入腔室的生物材料样品收集目标材料，其中微流体结构洗涤收集目标材料的磁珠，并通过来自外部能量源的电磁波照射分离核酸 到磁珠。 微流体系统包括微流体装置; 使旋转体旋转的旋转操作单元; 以及照射电磁波的外部能量源。

公开(公告)号：US20120178182A1

公开(公告)日：2012-07-12

申请号：US13345134

申请日：2012-01-06

Applicant: Kui Hyun KIM ,  Beom Seok LEE

Inventor： Kui Hyun KIM ,  Beom Seok LEE

IPC: G01N21/75 ,  B01L3/00

CPC classification number: B01L3/502738 ,  B01L2300/1838 ,  B01L2300/1861 ,  B01L2400/0677 ,  G01N33/54366

Abstract: Provided are a micro-fluidic device having multiple reaction chambers to simultaneously detect a plurality of different analytes, and an analyte detection method using the same. The micro-fluidic device includes multiple reaction chambers containing a plurality of capture materials to be combined with different analytes, multiple channels connecting the multiple reaction chambers, and valves provided within the multiple channels to control fluid flowing through the channels.

Abstract translation: 提供了具有多个反应室以同时检测多种不同分析物的微流体装置和使用其的分析物检测方法。 微流体装置包括多个反应室，其包含多个捕获材料以与不同的分析物组合，连接多个反应室的多个通道以及设置在多个通道内的阀，以控制流过通道的流体。

公开(公告)号：US08191715B2

公开(公告)日：2012-06-05

申请号：US12056345

申请日：2008-03-27

Applicant: Yoon-kyoung Cho ,  Beom-seok Lee

Inventor： Yoon-kyoung Cho ,  Beom-seok Lee

IPC: B01D17/038 ,  B01L3/00 ,  G01N21/07

CPC classification number: B01L3/502738 ,  B01L3/502753 ,  B01L2200/0684 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/0677 ,  G01N21/07 ,  Y10T436/111666 ,  Y10T436/2575

Abstract: A centrifugal force-based microfluidic device in which a sample including particles and a fluid is centrifugally separated such that the separated fluid is quantitatively distributed, and a microfluidic system including the centrifugal force-based microfluidic device are provided. The centrifugal force-based microfluidic device includes a microfluidic structure in which, within a rotatable disc-shaped platform, a sample, including particles and a fluid, is quickly centrifugally separated into the particles and the fluid using the rotation of the disc-shaped platform and the fluid having a certain volume of the separated fluid is discharged by rotation of the disc-shaped platform.

Abstract translation: 离心力微流体装置，其中包括颗粒和流体的样品被离心分离，使得分离的流体定量分布，并且提供包括基于离心力的微流体装置的微流体系统。 基于离心力的微流体装置包括微流体结构，其中在可旋转的盘形平台内，包括颗粒和流体的样品通过盘形平台的旋转快速离心分离成颗粒和流体 并且具有一定体积的分离流体的流体通过盘形平台的旋转而被排出。

公开(公告)号：US20120028830A1

公开(公告)日：2012-02-02

申请号：US13269297

申请日：2011-10-07

Applicant: Beom-seok LEE ,  Yoon-kyoung CHO ,  Jong-myeon PARK ,  Jeong-gun LEE

Inventor： Beom-seok LEE ,  Yoon-kyoung CHO ,  Jong-myeon PARK ,  Jeong-gun LEE

IPC: C40B30/04 ,  G01N33/566 ,  C40B60/12 ,  G01N30/96

CPC classification number: B01L3/502738 ,  B01L3/502723 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0677 ,  B01L2400/084 ,  B01L2400/086 ,  F16K99/0001 ,  F16K99/0019 ,  F16K99/0032 ,  F16K99/004 ,  F16K99/0061 ,  F16K2099/0084 ,  G01N35/00069

Abstract: Provided is a rotatable microfluidic device for conducting simultaneously two or more assays. The device includes a platform which can be rotated, a first unit which is disposed at one portion of the platform and detects a target material from a sample using surface on which a capture probe selectively binds to the target material is attached, and a second unit which is disposed at another portion of the platform and detects a target material included in the sample by a different reaction from the reaction conducted in the first unit.

公开(公告)号：US07988915B2

公开(公告)日：2011-08-02

申请号：US12115572

申请日：2008-05-06

Applicant: Beom-seok Lee ,  Jeong-gun Lee ,  Jong-myeon Park ,  Yoon-kyoung Cho

Inventor： Beom-seok Lee ,  Jeong-gun Lee ,  Jong-myeon Park ,  Yoon-kyoung Cho

IPC: C40B60/12

CPC classification number: B01L3/502738 ,  B01L3/502707 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0621 ,  B01L2200/16 ,  B01L2300/04 ,  B01L2300/0636 ,  B01L2300/0806 ,  B01L2400/0409 ,  B01L2400/0677 ,  Y10T436/15

Abstract: Disclosed is a microfluidic device including a microfluidic structure formed in a platform in which various examinations, such as an immune serum examination, can be automatically performed using the biomolecule microarray chip. The biomolecule microarray chip-type microfluidic device using a biomolecule microarray chip comprises: a platform which is rotatable; a microfluidic structure disposed in the platform, comprising: a plurality of chambers; a plurality of channels connecting the chambers each other; and a plurality of valves controlling flow of fluids through the channels, wherein the microfluidic structure controls flow of a fluid sample using rotation of the platform and the valves; and a biomolecule microarray chip mounted in the platform such that biomolecule capture probes bound to the biomolecule microarray chip contact the fluid sample in the microfluidic structure.

Abstract translation: 公开了一种微流体装置，其包括在平台中形成的微流体结构，其中可以使用生物分子微阵列芯片自动执行各种检查，例如免疫血清检查。 使用生物分子微阵列芯片的生物分子微阵列芯片型微流器件包括：可旋转的平台; 设置在平台中的微流体结构，包括：多个室; 连接所述室的多个通道; 以及多个阀，其控制通过所述通道的流体流动，其中所述微流体结构通过所述平台和所述阀的旋转来控制流体样品的流动; 以及安装在平台中的生物分子微阵列芯片，使得与生物分子微阵列芯片结合的生物分子捕获探针与微流体结构中的流体样品接触。

公开(公告)号：US07951332B2

公开(公告)日：2011-05-31

申请号：US12029480

申请日：2008-02-12

Applicant: Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

Inventor： Yoon-kyoung Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Young-sun Lee

IPC: B01L11/00

CPC classification number: B01L3/502738 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677

Abstract: Provided are a centrifugal force based microfluidic device which can automatically perform a dilution operation and a microfluidic system including the same. The centrifugal force based microfluidic device for dilution includes a rotatable disk type platform, a mixing chamber disposed on the platform; a buffer solution storage disposed on a portion of the platform which is closer to a center of the platform than the mixing chamber, connected to the mixing chamber through a channel to supply a predetermined amount of buffer solution to the mixing chamber at least one time, and a plurality of diluted solution chambers which are disposed on a portion of the platform which is farther from the center of the platform than the mixing chamber, are each connected to the mixing chamber through flow paths extended from a middle exit corresponding to a predetermined water level, and sequentially receiving a solution which is serially diluted in the mixing chamber at least one time.

Abstract translation: 提供了一种能够自动执行稀释操作的离心力微流体装置和包括其的微流体系统。 用于稀释的基于离心力的微流体装置包括可旋转盘式平台，设置在平台上的混合室; 缓冲溶液储存器，其设置在所述平台的比混合室更靠近所述平台的中心的部分上，所述缓冲溶液存储器经由通道至少一次向所述混合室提供预定量的缓冲溶液，所述通道连接到所述混合室， 并且多个稀释溶液室通过从对应于预定水的中间出口延伸的流路连接到平台的比混合室更远离平台中心的部分上的稀释溶液室 至少一次连续地接收在混合室中连续稀释的溶液。

公开(公告)号：US07926514B2

公开(公告)日：2011-04-19

申请号：US11766806

申请日：2007-06-22

Applicant: Jong-myeon Park ,  Yoon-kyuong Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Sung-woo Hong

Inventor： Jong-myeon Park ,  Yoon-kyuong Cho ,  Jeong-gun Lee ,  Beom-seok Lee ,  Sung-woo Hong

IPC: F15C1/04

CPC classification number: F16K99/0019 ,  B01L3/502738 ,  B01L2300/0803 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0032 ,  F16K99/0034 ,  F16K99/004 ,  F16K2099/0084 ,  Y10T137/1827 ,  Y10T137/2196 ,  Y10T137/6606 ,  Y10T137/7755

Abstract: Provided are a valve unit and a reaction apparatus having the valve unit. The valve unit includes a phase transition material, which melts and expands upon an application of the electromagnetic waves to the valve filler, and the valve filler is directed into the channel through the connection passage and closes the channel. The valve unit also includes heat generation particles, which are dispersed in the phase transition material and generate heat upon an application of electromagnetic wave energy.

Abstract translation: 提供具有阀单元的阀单元和反应装置。 阀单元包括相变材料，其在将电磁波施加到阀填充器时熔化和膨胀，并且阀填料通过连接通道引导到通道中并关闭通道。 阀单元还包括发散颗粒，其分散在相变材料中并且在施加电磁波能量时产生热量。

公开(公告)号：US20110070603A1

公开(公告)日：2011-03-24

申请号：US12862138

申请日：2010-08-24

Applicant: Ji Won KIM ,  Beom Seok LEE ,  In Wook KIM ,  Kui Hyum KIM

Inventor： Ji Won KIM ,  Beom Seok LEE ,  In Wook KIM ,  Kui Hyum KIM

IPC: C12Q1/02 ,  C12M1/34 ,  C12M1/38

CPC classification number: G01N33/487

Abstract: Disclosed are a blood tester for measurement of temperature of a sample placed in a blood-testing disc using a thermochromic pigment, and a control method thereof. The disclosed blood-testing disc, the blood tester having the blood-testing disc loaded therein, and the control method of the same, are used to measure a light absorbance of the thermochromic pigment in order to determine a temperature of a sample when a biological material such as blood, blood serum, blood plasma, or sputum, is subjected to analysis, so that the test may be executed at a constant temperature. Accordingly, more reproducible and accurate results are obtainable, and temperature measurement of a sample may be simply and effectively attained by an optical analyzer built in the blood tester, without an alternative instrument or sensor for measurement thereof.

Abstract translation: 公开了使用热变色颜料测量放置在血液测试盘中的样品的温度的血液测试仪及其控制方法。 所公开的血液测试盘，其中装有血液测试盘的血液测试仪及其控制方法用于测量热致变色素的吸光度，以便当生物学 对血液，血清，血浆，痰等材料进行分析，可以在恒温下进行试验。 因此，可以获得更多的可再现和准确的结果，并且可以通过内置在血液测试仪中的光学分析器简单有效地获得样品的温度测量，而不需要用于测量的替代仪器或传感器。

公开(公告)号：US20110020194A1

公开(公告)日：2011-01-27

申请号：US12851819

申请日：2010-08-06

Applicant: Beom Seok LEE ,  Yoon Kyoung CHO ,  Jeong Gun LEE ,  Jong-myeon PARK

Inventor： Beom Seok LEE ,  Yoon Kyoung CHO ,  Jeong Gun LEE ,  Jong-myeon PARK

IPC: G01N33/53 ,  G01N21/75

CPC classification number: B01L3/502738 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0442 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  G01N33/54326 ,  G01N33/54366 ,  Y10T436/25375

Abstract: A centrifugal force-based microfluidic device for the detection of a target biomolecule and a microfluidic system including the same are provided. The device includes a body of revolution; a microfluidic structure disposed in the body of revolution including chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structures transmitting fluid using centrifugal force due to rotation of the body of revolution; and beads disposed in the microfluidic structures, the beads having capture probes on the surfaces thereof which are selectively bonded with target protein; and a detection probe disposed in the microfluidic structures and selectively bonded to the target protein, and which includes a material required to express an optical signal, wherein the microfluidic structure mixes the beads, biological samples, and the detection probe to react and washes and separates the beads after the reaction.

Abstract translation: 提供了用于检测靶生物分子的离心力微流体装置和包括其的微流体系统。 该装置包括一个革命体; 设置在旋转体中的微流体结构，包括腔室，连接腔室的通道，以及设置在通道中的阀，以控制流体流动，微流体结构由于旋转体旋转而使用离心力传送流体; 和珠子，其设置在微流体结构中，珠粒在其表面上具有与靶蛋白质选择性结合的捕获探针; 以及设置在微流体结构中并选择性地结合到靶蛋白上并且包括表达光信号所需的材料的检测探针，其中微流体结构混合珠，生物样品和检测探针以反应和洗涤和分离 反应后的珠子。

公开(公告)号：US07758810B2

公开(公告)日：2010-07-20

申请号：US11875973

申请日：2007-10-22

Applicant: Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Jung-suk Yoo

Inventor： Jeong-gun Lee ,  Beom-seok Lee ,  Jong-myeon Park ,  Jung-suk Yoo

IPC: G01N21/07 ,  G01B11/00

CPC classification number: G01N35/00069 ,  B01L3/5027 ,  B01L2300/0654 ,  B01L2300/0803 ,  B01L2400/0409 ,  G01N35/0092 ,  G01N2035/00574

Abstract: Provided is a centrifugal force based microfluidic system including: a microfluidic device including a rotatable platform and an optical path formed to extend horizontally in a straight line from a circumference of the platform; a motor rotating so as to control the microfluidic device; a light emitting unit emitting light towards the microfluidic device; a light receiving unit detecting the light emitted from the light emitting unit; and a controller determining a home position to be the position of the microfluidic device at a point of time when the light emitted from the light emitting unit is detected by the light receiving unit, wherein the light emitted from the light emitting unit passes through the optical path to be incident on the light receiving unit only when the microfluidic device is located in a predetermined position.

Abstract translation: 提供了一种基于离心力的微流体系统，包括：微流体装置，其包括可旋转平台和形成为从平台的圆周以直线水平延伸的光路; 旋转的马达以控制微流体装置; 向所述微流体装置发射光的发光单元; 光接收单元，检测从所述发光单元发射的光; 以及控制器，其在从所述光接收单元检测到从所述发光单元发射的光的时刻，将所述微流体装置的位置确定为所述位置，其中，从所述发光单元发射的光通过所述光 仅当微流体装置位于预定位置时入射在光接收单元上的路径。