公开(公告)号：US20240286066A1

公开(公告)日：2024-08-29

申请号：US18692700

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Scott T. Hanks ,  Steven R. KJAR

IPC: B01D19/00

CPC classification number: B01D19/0068 ,  B01D19/0063

Abstract: A bubble trap includes a body having an interior surface bounding a cavity that extends between an upper end and an opposing lower end, an axis extends centrally through the cavity of the body between the upper end and the lower end, a first plane extends through the body orthogonal to the axis so as to divide the body into an upper body portion and a lower body, the interior surface of the lower body portion having a constant first radius from a fixed first center point, the interior surface of the upper body portion not having a constant radius from a fixed center point. A tubular spout projects into the cavity at the lower end encircles a channel that communicates with the cavity. A liquid inlet port, a liquid outlet port, and a gas outlet port communicate with the cavity.

公开(公告)号：US12070731B2

公开(公告)日：2024-08-27

申请号：US17505075

申请日：2021-10-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin Brenan ,  Jamie Cho ,  Javier Garcia ,  Robert Hess ,  Tanya Kanigan ,  Arrin Katz ,  Namyong Kim ,  John Linton ,  Shailesh Srivastava ,  Karl Yoder

IPC: B01L3/00 ,  B01J19/00 ,  B01L3/02

CPC classification number: B01J19/0046 ,  B01L3/50857 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L3/0244 ,  B01L3/5025 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16

Abstract: A method of registering a location of a dispenser array in relation to a microfluidic array is provided. One of the dispenser array and the microfluidic array can be movable in relation to the frame, and the other can be fixed relative to the frame. Their relative positions can be identified by a set of coordinates. Identification of a fiducial marker can occur in a manner permitting the fiducial reference to appear in a first position of a field of view of a first camera when the dispenser array or the microfluidic array is in an alignment position. Quantities related to a vector displacement from the alignment position to a fixed position on the microfluidic array or the dispenser array can be identified. Quantities determined can be used to guide positioning of the dispenser array relative to the microfluidic array.

公开(公告)号：US20240269628A1

公开(公告)日：2024-08-15

申请号：US18590633

申请日：2024-02-28

Applicant: Life Technologies Corporation

Inventor： Jeremy K. Larsen ,  Clinton C. Staheli ,  Brandon M. Knudsen

IPC: B01F27/2121 ,  B01F27/191 ,  B01F27/213 ,  B01F27/80 ,  B01F27/88 ,  B01F27/91 ,  B01F33/00 ,  B01F35/31 ,  B01F35/32 ,  B01F35/40 ,  B01F35/41 ,  B01F35/50 ,  B01F35/513

CPC classification number: B01F27/2121 ,  B01F27/191 ,  B01F27/213 ,  B01F27/88 ,  B01F27/91 ,  B01F33/86 ,  B01F35/322 ,  B01F35/3231 ,  B01F35/40 ,  B01F35/4121 ,  B01F35/513 ,  B01F27/80 ,  B01F35/31 ,  B01F35/41 ,  B01F35/4112 ,  B01F35/514

Abstract: A fluid mixing system includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment; a retainer coupled to the second end of the flexible bag, the retainer having a retention cavity formed thereon that communicates with the compartment of the flexible bag; and an elongated member having a first end rotatably coupled to the first end of the flexible bag and an opposing second end freely disposed within the retention cavity of the retainer so that the second end of the elongated member can rotate within the retention cavity relative to the retainer.

公开(公告)号：US20240263168A1

公开(公告)日：2024-08-08

申请号：US18636580

申请日：2024-04-16

Applicant: Life Technologies Corporation

Inventor： Chiu Tai Andrew Wong ,  Mark Beauchemin ,  Shanti Shankar ,  Kylan Szeto

IPC: C12N15/10 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/10

CPC classification number: C12N15/1065 ,  C12Q1/6825 ,  C12Q1/6874 ,  G01N35/1081

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US20240238789A1

公开(公告)日：2024-07-18

申请号：US18545690

申请日：2023-12-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Earl Zergiebel

IPC: B01L3/00 ,  B01L3/02 ,  B01L9/00

CPC classification number: B01L3/527 ,  B01L3/0293 ,  B01L3/50855 ,  B01L3/523 ,  B01L9/543 ,  B01L2300/0825 ,  B01L2300/0858

Abstract: A reagent container includes a base defining a plurality of wells having openings exposed at an upper surface of the base and a tube receptacle. The plurality of wells include a first set of wells and a second set of wells. Each well of the first set of wells has an opening to a well bore and a channel in communication with the well bore. The opening has a first portion disposed over the well bore and a second portion disposed over the channel. The first portion has a larger area than the second portion. An angle defined by tangents to the inner surface of the first and second portions at a junction between the first and second portions is at least 100° and not greater than 180°. The reagent container further includes a top coupled over the top surface of the base and defining windows providing access to the openings of the plurality of wells and the tube receptacle.

公开(公告)号：US20240228785A1

公开(公告)日：2024-07-11

申请号：US18419897

申请日：2024-01-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen HU ,  Aimei CHEN ,  Daniel BEACHAM ,  Chrisgen VONNEGUT ,  Krishnamurthy NACHARAJU

IPC: C09B11/24 ,  G01N21/64

CPC classification number: C09B11/24 ,  G01N21/6428 ,  G01N2021/6439

Abstract: The present disclosure provides for compounds of Formula (I),




its corresponding compounds of Formula (II)




or salts thereof and their use as fluorogenic pH sensors.

公开(公告)号：US12031916B2

公开(公告)日：2024-07-09

申请号：US17962123

申请日：2022-10-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mingsong Chen ,  Mauro Aguanno ,  Kuan Moon Boo ,  Woon Liang Terence Soh ,  Jacob K. Freudenthal ,  Jeffrey A. Marks

IPC: G01N21/64 ,  G01N21/03 ,  G01N21/27

CPC classification number: G01N21/6486 ,  G01N21/0332 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2201/061 ,  G01N2201/0624 ,  G01N2201/0627 ,  G01N2201/121

Abstract: An instrument for processing and/or measuring a biological process comprises a sample processing system and an excitation source exhibiting a spectral function of output power or intensity verses wavelength of output power or intensity. The spectral function has a minima wavelength corresponding to a local minima value of the output power or intensity; a first maxima wavelength corresponding to a first local maxima of output power or intensity, the output power or intensity at the first local maxima being greater than the output power or intensity at any wavelength less than the minima wavelength; a second maxima wavelength corresponding to a second local maxima of output power or intensity, the output power or intensity at the second local maxima being greater than the output at any wavelength greater than the minima wavelength; the minima wavelength is between the first maxima wavelength and the second maxima wavelength.

公开(公告)号：US20240219388A1

公开(公告)日：2024-07-04

申请号：US18391779

申请日：2023-12-21

Applicant: Pierce Biotechnology, Inc. ,  Life Technologies Corporation ,  Bender MedSystems GmbH

Inventor： Paul Haney ,  Surbhi Desai ,  Marie Murakami ,  Penny Jensen ,  Brian Steer ,  Kelli Feather-Henigan ,  Syrus Jaffe ,  Brian Webb ,  Korin Pathammavong ,  Ricarda Heintz ,  Jordan Thompson ,  Jeremy Weaver ,  Carina Wimer ,  Andrew Westergren ,  Kai Chun Chen

IPC: G01N33/573 ,  C12N15/10 ,  G01N1/34 ,  G01N33/68

CPC classification number: G01N33/573 ,  C12N15/101 ,  G01N1/34 ,  G01N33/68 ,  G01N2333/90203 ,  G01N2333/98

Abstract: Methods for fractionating a sample of biomolecules of the disclosure comprise (a) introducing a sample into a separating column; (b) separating biomolecules in the sample by molecular weight into a plurality of fractions along the separating column; and (c) placing the separating column into successive engagement with a plurality of wells and advancing into each of the wells one or more of the corresponding plurality of fractions. Methods of the disclosure further comprise detecting and identifying biomolecules in the fractions using microparticles to bind to the fractions, and novel approaches to pool the fractions followed by assays—such as immunoassays—that allow high throughput and automation capabilities.

公开(公告)号：US20240201126A1

公开(公告)日：2024-06-20

申请号：US18536131

申请日：2023-12-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James BUSTILLO ,  Mark J. MILGREW ,  Wolfgang HINZ ,  John LEAMON ,  John DAVIDSON ,  Martin HUBER ,  Antoine M. VAN OIJEN ,  Jonathan ROTHBERG

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6874 ,  H01L21/82

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11998910B2

公开(公告)日：2024-06-04

申请号：US17412376

申请日：2021-08-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Reed ,  Zheng (Derek) Xing ,  David Marran ,  Earl Zergiebel

IPC: B01L3/00 ,  C12Q1/6874

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  C12Q1/6874 ,  B01L2200/026 ,  B01L2200/06 ,  B01L2300/0609 ,  B01L2300/0636 ,  B01L2300/0848

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.