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    Methods and compositions for high protein production from non-native DNA
    31.
    发明授权
    Methods and compositions for high protein production from non-native DNA 失效
    用于从非天然DNA产生高蛋白质的方法和组合物

    公开(公告)号：US5733782A

    公开(公告)日：1998-03-31

    申请号：US464589

    申请日：1995-06-05

    Applicant: Haimanti Dorai ,  Hermann Oppermann

    Inventor： Haimanti Dorai ,  Hermann Oppermann

    IPC: C07K16/00 ,  C07K16/32 ,  C12N15/13 ,  C12N15/85 ,  C12P21/08 ,  C12N5/10

    CPC classification number: C07K16/32 ,  C07K16/00 ,  C12N15/85 ,  C12N2799/022 ,  C12N2830/002 ,  C12N2830/55 ,  C12N2840/20 ,  Y10S530/867

    Abstract: Disclosed herein are improved methods and compositions for achieving enhanced protein production expressed from non-native gene constructs, including single chain sFv and derivative sequences. The methods and compositions are particularly useful for creating stably transfected, constitutively expressing immortalized mammalian cell lines that exhibit high recombinant protein productivity while maintaining a low copy number per cell of the non-native recombinant DNA sequence encoding the protein of interest.

    Abstract translation: 本文公开了用于实现由非天然基因构建体（包括单链sFv和衍生序列）表达的增强的蛋白质产生的改进的方法和组合物。 所述方法和组合物特别可用于产生稳定转染的组成型表达的永生化哺乳动物细胞系，其表达高重组蛋白质生产力，同时保持编码目的蛋白质的非天然重组DNA序列的每个细胞的拷贝数低。

    DNA encoding a protein which enables selective removal of immune complexes
    32.
    发明授权
    DNA encoding a protein which enables selective removal of immune complexes 失效
    编码能够选择性去除免疫复合物的蛋白质的DNA

    公开(公告)号：US5243040A

    公开(公告)日：1993-09-07

    申请号：US787669

    申请日：1991-11-04

    Applicant: James S. Huston ,  Lynn Baird ,  Charles Cohen ,  Hermann Oppermann

    Inventor： James S. Huston ,  Lynn Baird ,  Charles Cohen ,  Hermann Oppermann

    IPC: A61M1/36 ,  C07K14/31 ,  C07K17/06 ,  C12N15/31

    CPC classification number: A61M1/3679 ,  C07K14/31 ,  C07K17/06

    Abstract: Disclosed is a method and a family of materials useful for removing immune complexes from blood preferentially to soluble antibodies. The material comprises analogs of proteins which bind to the Fc region of immunoglobulin. The analogs are produced by truncating or otherwise altering the amino acid sequence of the binding protein to reduce their affinity for Fc. An array of such analogs disposed about the surface of an insoluble matrix has the ability to form multiple points of attachment to the multiple Fc's in a complex so as to bind complex strongly, whereas only weak associations are developed between the Fc region of soluble IgG and individual analogs. The preferred analogs are truncated proteins homologous to a portion of the domains of Protein A or Protein G which bind with Fc. Complex may be removed from whole blood or serum using the material and conventional plasmapheresis techniques.

    Abstract translation: 公开了一种用于从血液中将免疫复合物优先地去除可溶性抗体的方法和一系列材料。 该材料包含与免疫球蛋白Fc区结合的蛋白质的类似物。 类似物通过截短或以其它方式改变结合蛋白的氨基酸序列来降低它们对Fc的亲和力而产生。 在不溶性基质的表面附近设置的这种类似物的阵列具有在复合物中形成与多个Fc的多个连接点的能力，以便强烈地结合复合物，而仅在可溶性IgG的Fc区和 个别类似物。 优选的类似物是与蛋白A或蛋白G的结合部分与Fc结合的截短的蛋白质。 可以使用材料和常规的血浆置换技术从全血或血清中除去复合物。

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